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鱼塘沉积物中氯霉素的超声萃取、净化与测定 被引量:6
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作者 吕玄文 梁敏思 +1 位作者 党志 杨琛 《农业环境科学学报》 CAS CSCD 北大核心 2007年第3期1195-1200,共6页
通过对鱼塘沉积物中氯霉素的提取、净化洗脱和测定方法研究,尝试了以甲醇为萃取剂,利用超声萃取鱼塘沉积物中的氯霉素,然后再以层析柱净化,甲醇作洗脱液洗脱,用高效液相色谱法(HPLC)分析氯霉素含量。结果表明,当用甲醇超声萃取3次(30、1... 通过对鱼塘沉积物中氯霉素的提取、净化洗脱和测定方法研究,尝试了以甲醇为萃取剂,利用超声萃取鱼塘沉积物中的氯霉素,然后再以层析柱净化,甲醇作洗脱液洗脱,用高效液相色谱法(HPLC)分析氯霉素含量。结果表明,当用甲醇超声萃取3次(30、10和10min),并用甲醇作层析柱净化洗脱液时,提取效果比较理想。利用HPLC分析氯霉素含量的色谱条件为:HpersilODS色谱柱4.6mm×250mm,进样量10μL,流速1.0mL·min-1,柱温30℃,检测波长275nm,流动相为乙腈:水=60:40(V/V)。此方法的检测限为7μg·kg-1,平均回收率为82.74%~88.38%,精密度RSD为0.4%~3.3%。应用该方法检测得到食用鱼塘和金鱼鱼塘沉积物中氯霉素的残留量分别为0.1716和0.1224mg·kg-1。 展开更多
关键词 氯霉素 鱼塘沉积物 超声萃取 净化 HPLC
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Microplastics in Freshwater Aquaculture Fishpond System in Yichang,China:The Occurrence,Characteristics and Potential Risks
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作者 JIA Manke XU Wenrui +4 位作者 LIU Qin ZHOU Ljunjun SHI Xiaotao TAN Hui LIU Liming 《Wuhan University Journal of Natural Sciences》 CAS 2024年第5期471-483,共13页
At present,microplastics have attracted much concern worldwide,but still few researches have focused especially on aquaculture fishponds.In this study,investigations were conducted in Zhijiang(ZJ)and Yidu(YD)areas of ... At present,microplastics have attracted much concern worldwide,but still few researches have focused especially on aquaculture fishponds.In this study,investigations were conducted in Zhijiang(ZJ)and Yidu(YD)areas of Hubei,China,typical freshwater aquaculture bases of the middle and lower reaches of the Yangtze River,on the occurrence and distribution characteristics of microplastics in fishpond system.It was found that microplastics presented in all samples from water,sediment,fish,and fish feed.Most microplastics detected had a diameter of<1.0 mm,blue and black were the most common colors,and fiber shape was the dominant type.The most common polymer types were polyethylene(PE)and polypropylene(PP).Microplastic abundance in fish was correlated with that in sediment and water.The potential ecological risk index values indicated that water,sediment,and fish samples were under moderate pollution risk.The results of this study illustrated the microplastics pollution situation in freshwater cultured fishpond systems,and the findings presented here will serve as a reference for future investigations of the environmental risks of microplastics in aquaculture environment. 展开更多
关键词 microplastic fishpond aquaculture fish feed sediment ecological risk assessment
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