[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR (Yangtze River stock ♀×Yangtze River...[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR (Yangtze River stock ♀×Yangtze River stock ♂ ), ZR (Zhujiang River stock ♀ ×Zhujiang River stock♂ ) and hybrids F1 ( Yangtze River stock ♀ × Zhujiang River stock ♂ ) were established, and their growth or morphological data were also measured. [ Result] The orders of body weight, standard length and absolute growth rate were F1 〉 ZR 〉 YR. No significant difference was found in body weight or standard length of 50 day-old generations ( P〉0. 05), while there was an extremely significantly difference in those of 170 day-old generations ( P 〈0.01 ). However, the absolute growth rate of F1 was 20.00% and 50.00% higher than that of ZR and YR respectively, and no significant difference was found between F1 and ZR ( P 〉 0.05), but significant difference between F1 and YR ( P 〈 0.05). F1 showed a significant hybrid vigor with rate of 20.09%. There was no significant difference among three inbred generations in standard length/total length ( P 〉 0.05), while significant difference in head length/total length, body height/standard length and body width/standard length ( P 〈 0.05), which indicated that F1 had the characteristics of shorter head, higher and wider body. [ Conclusion]F1 has advantages in growth performance and morphological characteristics.展开更多
[Objective] This study aimed to evaluate growth performance of hybrid progenies of 10 grass carp (Ctenopharyngodon idella) populations from different ge- ographical origins (Foshan population, Zhaoqing population, ...[Objective] This study aimed to evaluate growth performance of hybrid progenies of 10 grass carp (Ctenopharyngodon idella) populations from different ge- ographical origins (Foshan population, Zhaoqing population, Jingzhou population 1, Jingzhou population 2, Jingzhou population 3, Ezhou population, Yiyang population, Changsha population 1, Changsha population 2 and Jiangsu population). [Method] Fifteen hybrid combinations were established with these ten grass carp populations and cultured in the same pond. The body mass of hybrid progenies was measured at the age of 4, 7, 8, 10, 12, 15 and 18 months, respectively. Using body mass at the age of 4 months as a concomitant variable, the growth performance of proge- nies from different hybrid combinations was analyzed by covariance analysis. [Re- suit] The average body mass of hybrid progenies of Jingzhou population 2 c2 x Foshan population $ reached the maximum (1 892.90 g) at the age of 18 months, which was 3.51%-32.36% higher than that of other hybrid progenies. Multiple com- parison analysis showed that average body mass of hybrid progenies of Jingzhou population 2 2 x Foshan population ♂ and Jingzhou population 1 ♀× Foshan population ♂ was significantly higher than that of other hybrid progenies (P〈0.05). [Conclusion] In the production process, Jingzhou population 2 ♀ × Foshan popula- tion 5 and Jingzhou population 1 ♀×Foshan population ♂ can be used to pro- duce high-quality fries, thereby greatly improving economic benefits. This study laid the foundation for establishment of rapid-growing grass carp core populations.展开更多
The self-incompatibility ( S) loci from the Solanaceae, Rosaceae and Scrophulariaceae encode a class of ribonucleases, known as S RNases, which have been shown to control the pistil expression of self-incompatible rea...The self-incompatibility ( S) loci from the Solanaceae, Rosaceae and Scrophulariaceae encode a class of ribonucleases, known as S RNases, which have been shown to control the pistil expression of self-incompatible reaction. In the former two families, the S loci have been shown to be located near centromere. However, the chromosomal location of the S locus in Antirrhinum, a species of the Scrophulariaceae, is not known. To determine its chromosomal location and genomic organization, an S-2 RNase gene and its corresponding 63 kb BAC clone were separately used for fluorescence in situ hybridization (FISH) of mitotic metaphase chromosomes of a self-incompatible Antirrhinum line Of S2S5. The results showed that the S-2 RNase detected a doublet signal near the centromere of the smallest chromosome (2n = 16). Two separate doublet signals of the tested BAC sequence were shown on both sides of the centromeres of all eight pairs of the chromosomes, suggesting that the Antirrhinum S locus is located in a pericentromeric region. Furthermore, a retrotransposon, named RIS1 (retrotransposon in the S locus), which has not been identified yet in. Antirrhinum, was found next to S-2 RNase. Taken together, the centromeric location of the S locus from the three S-RNase-based self-incompatible families provides a further support on a common origin of their evolution as well as suppressed recombination.展开更多
[Objective] This study aimed to observe the embryonic development of the F1 hybrid between Ryukin (♀) and Dragon-eye (♂). [Method] Embryonic development of the FI hybrid and parents between Ryukin (♀) and Dra...[Objective] This study aimed to observe the embryonic development of the F1 hybrid between Ryukin (♀) and Dragon-eye (♂). [Method] Embryonic development of the FI hybrid and parents between Ryukin (♀) and Dragon-eye (♂) was observed with XTL-2400 anatomical lens continuously. The organ characteristics and structures at each development stage were observed with OLYMPUS CKX41 microscopes, and photographed and recorded with a digital camera. [Result] At a water temperature of 25℃, the hatching time of the F1 hybrid is 51.3 h, the hatching time of Ryukin embryo is 52.4 h and that of Dragon-eye embryo is 56.3 h. [Conclusion] This study provides certain theoretical and practical basis for improving morphological characteristics of the existing Dragon-eye goldfish.展开更多
Hybrid tilapia were reared at densities of 1, 5 or 10 fish per tank for four weeks. Mortality was 0 at 1 and 10 fish per tank, but was 25% at 5 fish per tank. Specific growth rate was highest at 1 fish per tank, and l...Hybrid tilapia were reared at densities of 1, 5 or 10 fish per tank for four weeks. Mortality was 0 at 1 and 10 fish per tank, but was 25% at 5 fish per tank. Specific growth rate was highest at 1 fish per tank, and lowest at 5 fish per tank. The lower growth rate at the intermediate stocking density was associated with reduced feed efficiency, but there was no reduction in feed intake or digestibility. The results suggested increased metabolic cost caused by aggressive behaviour at intermediate stocking density, which can be suppressed by a further increase in density.展开更多
The Labeo dyocheilus(family Cyprinidae)in-habits fresh water streams at the fo thill regions and is a commercially important food fish.It is native to Afganistan,Pakistan,India,Nepal,Bhutan,Bangladesh,M yanmnar,Cambod...The Labeo dyocheilus(family Cyprinidae)in-habits fresh water streams at the fo thill regions and is a commercially important food fish.It is native to Afganistan,Pakistan,India,Nepal,Bhutan,Bangladesh,M yanmnar,Cambodia and Thailand(Froese and Pauly,2003).In India,the species in-habits the Indus,Ganges and Mahanadi river sys-terns.It is identified as a potential cultivable species and has considerable significance for the fishery of these regions.L.dyocheilus has been categorized as a vulnerable fish due to declining commercial catches in India(Mahanta et a1.,1994).At present the fishery is supported through capture from natural sources.To achieve domestication,various aspects of captive breeding and propagation are being studied.展开更多
The developmental fate of the pronuclei in recombined embryos obtained by transplanting the donor nuclei into the non-enucleated eggs remains controversial in the case of fish. In the present study, the nuclei from th...The developmental fate of the pronuclei in recombined embryos obtained by transplanting the donor nuclei into the non-enucleated eggs remains controversial in the case of fish. In the present study, the nuclei from the loach blastulae were transplanted into non-enucleated zebrafish eggs, the resulting 9 inter-family nuclear transplant embryos developed to larval stages. Although the development timing of the nuclear transplants resembled that of zebrafish, chromosome examination revealed that most of the recombined embryos were diploids with karyotype characteristic of loach, which was also proved by RAPD analysis. Moreover, 3 out of the 9 larval fish formed barb rudiments specific to loach. It was therefore concluded that the nuclear transplant larval fish were inter-family nucleo-cytoplasmic hybrids; and that only the donor nuclei were involved in the development of the nuclear transplant embryos, while the pronuclei in the non-enucleated eggs were likely automatically eliminated during the development.展开更多
A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open read...A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind grit and testis, hepatic caecum, gill, endostyle, and epipbaryngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-NI/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.展开更多
基金Supported by National Freshwater Fish Industrial Technology System ProjectKey Discipline Construction Project of Shanghai(Y1101)Key Project of Science Committee in Shanghai(06DJ14003)~~
文摘[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR (Yangtze River stock ♀×Yangtze River stock ♂ ), ZR (Zhujiang River stock ♀ ×Zhujiang River stock♂ ) and hybrids F1 ( Yangtze River stock ♀ × Zhujiang River stock ♂ ) were established, and their growth or morphological data were also measured. [ Result] The orders of body weight, standard length and absolute growth rate were F1 〉 ZR 〉 YR. No significant difference was found in body weight or standard length of 50 day-old generations ( P〉0. 05), while there was an extremely significantly difference in those of 170 day-old generations ( P 〈0.01 ). However, the absolute growth rate of F1 was 20.00% and 50.00% higher than that of ZR and YR respectively, and no significant difference was found between F1 and ZR ( P 〉 0.05), but significant difference between F1 and YR ( P 〈 0.05). F1 showed a significant hybrid vigor with rate of 20.09%. There was no significant difference among three inbred generations in standard length/total length ( P 〉 0.05), while significant difference in head length/total length, body height/standard length and body width/standard length ( P 〈 0.05), which indicated that F1 had the characteristics of shorter head, higher and wider body. [ Conclusion]F1 has advantages in growth performance and morphological characteristics.
基金Supported by National Science and Technology Project in Rural Areas during the"12th Five-Year Plan"Bulk Breeding of New Varieties of Major Freshwater Fishes"(2012BAD26B02)Construction of National Bulk Freshwater Fish industry Technology System"Selective Breeding and Molecular-assisted Breeding of Grass Carp in South China"(CARS-46-03)~~
文摘[Objective] This study aimed to evaluate growth performance of hybrid progenies of 10 grass carp (Ctenopharyngodon idella) populations from different ge- ographical origins (Foshan population, Zhaoqing population, Jingzhou population 1, Jingzhou population 2, Jingzhou population 3, Ezhou population, Yiyang population, Changsha population 1, Changsha population 2 and Jiangsu population). [Method] Fifteen hybrid combinations were established with these ten grass carp populations and cultured in the same pond. The body mass of hybrid progenies was measured at the age of 4, 7, 8, 10, 12, 15 and 18 months, respectively. Using body mass at the age of 4 months as a concomitant variable, the growth performance of proge- nies from different hybrid combinations was analyzed by covariance analysis. [Re- suit] The average body mass of hybrid progenies of Jingzhou population 2 c2 x Foshan population $ reached the maximum (1 892.90 g) at the age of 18 months, which was 3.51%-32.36% higher than that of other hybrid progenies. Multiple com- parison analysis showed that average body mass of hybrid progenies of Jingzhou population 2 2 x Foshan population ♂ and Jingzhou population 1 ♀× Foshan population ♂ was significantly higher than that of other hybrid progenies (P〈0.05). [Conclusion] In the production process, Jingzhou population 2 ♀ × Foshan popula- tion 5 and Jingzhou population 1 ♀×Foshan population ♂ can be used to pro- duce high-quality fries, thereby greatly improving economic benefits. This study laid the foundation for establishment of rapid-growing grass carp core populations.
文摘The self-incompatibility ( S) loci from the Solanaceae, Rosaceae and Scrophulariaceae encode a class of ribonucleases, known as S RNases, which have been shown to control the pistil expression of self-incompatible reaction. In the former two families, the S loci have been shown to be located near centromere. However, the chromosomal location of the S locus in Antirrhinum, a species of the Scrophulariaceae, is not known. To determine its chromosomal location and genomic organization, an S-2 RNase gene and its corresponding 63 kb BAC clone were separately used for fluorescence in situ hybridization (FISH) of mitotic metaphase chromosomes of a self-incompatible Antirrhinum line Of S2S5. The results showed that the S-2 RNase detected a doublet signal near the centromere of the smallest chromosome (2n = 16). Two separate doublet signals of the tested BAC sequence were shown on both sides of the centromeres of all eight pairs of the chromosomes, suggesting that the Antirrhinum S locus is located in a pericentromeric region. Furthermore, a retrotransposon, named RIS1 (retrotransposon in the S locus), which has not been identified yet in. Antirrhinum, was found next to S-2 RNase. Taken together, the centromeric location of the S locus from the three S-RNase-based self-incompatible families provides a further support on a common origin of their evolution as well as suppressed recombination.
文摘[Objective] This study aimed to observe the embryonic development of the F1 hybrid between Ryukin (♀) and Dragon-eye (♂). [Method] Embryonic development of the FI hybrid and parents between Ryukin (♀) and Dragon-eye (♂) was observed with XTL-2400 anatomical lens continuously. The organ characteristics and structures at each development stage were observed with OLYMPUS CKX41 microscopes, and photographed and recorded with a digital camera. [Result] At a water temperature of 25℃, the hatching time of the F1 hybrid is 51.3 h, the hatching time of Ryukin embryo is 52.4 h and that of Dragon-eye embryo is 56.3 h. [Conclusion] This study provides certain theoretical and practical basis for improving morphological characteristics of the existing Dragon-eye goldfish.
文摘Hybrid tilapia were reared at densities of 1, 5 or 10 fish per tank for four weeks. Mortality was 0 at 1 and 10 fish per tank, but was 25% at 5 fish per tank. Specific growth rate was highest at 1 fish per tank, and lowest at 5 fish per tank. The lower growth rate at the intermediate stocking density was associated with reduced feed efficiency, but there was no reduction in feed intake or digestibility. The results suggested increased metabolic cost caused by aggressive behaviour at intermediate stocking density, which can be suppressed by a further increase in density.
基金This research was funded by agrant from ICAR(No NBFGR/DNA25A)
文摘The Labeo dyocheilus(family Cyprinidae)in-habits fresh water streams at the fo thill regions and is a commercially important food fish.It is native to Afganistan,Pakistan,India,Nepal,Bhutan,Bangladesh,M yanmnar,Cambodia and Thailand(Froese and Pauly,2003).In India,the species in-habits the Indus,Ganges and Mahanadi river sys-terns.It is identified as a potential cultivable species and has considerable significance for the fishery of these regions.L.dyocheilus has been categorized as a vulnerable fish due to declining commercial catches in India(Mahanta et a1.,1994).At present the fishery is supported through capture from natural sources.To achieve domestication,various aspects of captive breeding and propagation are being studied.
文摘The developmental fate of the pronuclei in recombined embryos obtained by transplanting the donor nuclei into the non-enucleated eggs remains controversial in the case of fish. In the present study, the nuclei from the loach blastulae were transplanted into non-enucleated zebrafish eggs, the resulting 9 inter-family nuclear transplant embryos developed to larval stages. Although the development timing of the nuclear transplants resembled that of zebrafish, chromosome examination revealed that most of the recombined embryos were diploids with karyotype characteristic of loach, which was also proved by RAPD analysis. Moreover, 3 out of the 9 larval fish formed barb rudiments specific to loach. It was therefore concluded that the nuclear transplant larval fish were inter-family nucleo-cytoplasmic hybrids; and that only the donor nuclei were involved in the development of the nuclear transplant embryos, while the pronuclei in the non-enucleated eggs were likely automatically eliminated during the development.
基金Supported by the Pilot Projects of Knowledge Innovation Project of Chinese Academy of Sciences (No. KZCX2-YW-211-03 and MGE2008KG06)
文摘A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind grit and testis, hepatic caecum, gill, endostyle, and epipbaryngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-NI/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.