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用酶联免疫吸附法测定鱼类体内金属硫蛋白 被引量:13
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作者 林芃 任宏伟 茹炳根 《环境污染与防治》 CAS CSCD 北大核心 2001年第5期265-267,共3页
用酶联免疫吸附法测定鱼体内 (肝 ,肾 ) MT。鱼组织经匀浆、加热、离心、将上清液以包被液稀释后 ,与一抗、二抗反应 ,求得 MT含量。与镉 /血红蛋白饱和法比较 ,本法显示了特异、简便、及灵敏的特点。同时 ,用酶联免疫吸附法测定了取自... 用酶联免疫吸附法测定鱼体内 (肝 ,肾 ) MT。鱼组织经匀浆、加热、离心、将上清液以包被液稀释后 ,与一抗、二抗反应 ,求得 MT含量。与镉 /血红蛋白饱和法比较 ,本法显示了特异、简便、及灵敏的特点。同时 ,用酶联免疫吸附法测定了取自武汉东湖不同水域的鱼类、不同种类的鱼类的组织脏器的 MT的含量 。 展开更多
关键词 金属硫蛋白 酶联免疫吸附法 特异性 鱼类体内 生物监测
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Pollution of Environmental Endocrine Disrupting Chemicals(EDCs) in Water and Its Adverse Reproductive Effect on Fish 被引量:3
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作者 孟顺龙 宋超 +3 位作者 范立民 裘丽萍 陈家长 徐跑 《Agricultural Science & Technology》 CAS 2014年第3期463-469,共7页
Environmental endocrine disrupting chemicals (EDCs), commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, ... Environmental endocrine disrupting chemicals (EDCs), commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, more and more EDCs were released into the environment. EDCs go into water body via atmosphere sedi-mentation, surface runoff, soil eluviation, etc., so water body becomes the main place for existing. In order to attract scientific and public attention worldwide and to prevent EDCs pol ution, in this study we reviewed the classification of EDCs and their concentrations in natural water bodies, drinking water sources and water plants, and the reproductive toxicity of EDCs to fish were reviewed. EDCs could disturb the endocrine system and make reproductive organs and reproduction abnor-mal, resulting in fertility descending, reproduction function damage, community quan-tity decrease and even species extinction. In addition, EDCs could disrupt the homeostasis maintained by hormones, which would result in defects of neural de-velopment and abnormalities of the endocrine and reproductive systems. The exact molecular mechanisms have not been completely reported, but researches have suggested that multiple mechanisms were involved in the action of EDCs. Although there have been researches on the biohazard of EDCs, there stil exist problems of weakness in fundamental researches, difficulties in recognizing and identifying EDCs and high cost, which restraint the knowledge on them. 展开更多
关键词 Environmental endocrine disrupting chemical Water body Pollution situ-ation FISH Damage of reproduction
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Bioaccumulation of Mercury in Fish Species from Different Trophic Level
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《Journal of Food Science and Engineering》 2017年第7期363-367,共5页
Mercury (Hg) is an environmental pollutant, and can bioaccumulate and biomagnify in the food web. Twenty four fish samples of different tropic level i.e., Herbivore, Omnivore and Carnivore were collected from two co... Mercury (Hg) is an environmental pollutant, and can bioaccumulate and biomagnify in the food web. Twenty four fish samples of different tropic level i.e., Herbivore, Omnivore and Carnivore were collected from two contaminated rivers, the Megna and the Buriganga which surround the Dhaka division for the assessment of toxic Hg. Fourteen samples were found to contain Hg in the range of 0.01-0.09 mg/kg. The highest Hg (0.09 mg/kg) was found in the Carnivore, Bele (Glossogobius giuris). However, all fish species had lower amount of Hg than the maximum Hg limit (0.5 mg/kg for fish) set by World Health Organization (WHO). Among all the fish species, the order of bioaccumulation was carnivore 〉 omnivore 〉 herbivore. Kajoli (Ailia coila), Shing (Heteropnuestes fossilis), Rui (Labeo rohita), Chewa (Tryauchen vagine), Rita (Rita rita), Hilsha (Tenualosa ilisha), Small Puti (Puntius sophore), Bacha (Eutropiichthys vacha) and Chingri (smallprawn) were not found to contain any Hg which indicated that fish species from these rivers are safe for human consumption. 展开更多
关键词 BIOACCUMULATION BIOMAGNIFICATION chemical contaminant food chain and Atomic Absorption Spectroscopy (AAS).
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