目的:运用准确快速的鱼类品种鉴定方法,对上海市售鱼类制品标识符合性进行调查。方法:利用DNA条形码技术,以动物线粒体细胞色素C氧化酶(Cytochrome C Oxidase Subunit I,COI)基因序列为鉴定靶标,对采集的63种市售鱼类样本进行序列分析...目的:运用准确快速的鱼类品种鉴定方法,对上海市售鱼类制品标识符合性进行调查。方法:利用DNA条形码技术,以动物线粒体细胞色素C氧化酶(Cytochrome C Oxidase Subunit I,COI)基因序列为鉴定靶标,对采集的63种市售鱼类样本进行序列分析比对后,分析样品的标注名称与真实物种名的一致性。结果:经序列测定和比对,13份样品的鱼类品种名称与标注名称不一致,占20.63%,此外有19份样品标注名为一类鱼的总称或俗称,占30.16%。结论:目前,我国对鱼类及其制品物种真实性鉴定研究亟待发展,鱼类标注物种名称混乱,分类不清,概念模糊,急需规范化。展开更多
The grass carp(Ctenopharyngodon idella) is one of the most important cultivated fish species in China.Mounting evidences suggests that microRNAs(miRNAs) may be key regulators of skeletal muscle among the grass carp,bu...The grass carp(Ctenopharyngodon idella) is one of the most important cultivated fish species in China.Mounting evidences suggests that microRNAs(miRNAs) may be key regulators of skeletal muscle among the grass carp,but the knowledge of the identity of myogenic miRNAs and role of miRNAs during skeletal muscle anabolic state remains limited.In the present study,we choose 8 miRNAs previously reported to act as muscle growth-related miRNAs for fasting-refeeding research.We investigated postprandial changes in the expression of 8 miRNAs following a single satiating meal in grass carp juveniles who had been fasting for one week and found that 7 miRNAs were sharply up-regulated within 1 or 3 h after refeeding,suggesting that they may be promising candidate miRNAs involved in a fast-response signaling system that regulates fish skeletal muscle growth.展开更多
文摘目的:运用准确快速的鱼类品种鉴定方法,对上海市售鱼类制品标识符合性进行调查。方法:利用DNA条形码技术,以动物线粒体细胞色素C氧化酶(Cytochrome C Oxidase Subunit I,COI)基因序列为鉴定靶标,对采集的63种市售鱼类样本进行序列分析比对后,分析样品的标注名称与真实物种名的一致性。结果:经序列测定和比对,13份样品的鱼类品种名称与标注名称不一致,占20.63%,此外有19份样品标注名为一类鱼的总称或俗称,占30.16%。结论:目前,我国对鱼类及其制品物种真实性鉴定研究亟待发展,鱼类标注物种名称混乱,分类不清,概念模糊,急需规范化。
基金Foundation items： This study was supported by the National Natural Science Foundation of China （31230076 31340054）, the Natural Science Foundation of Hunan province （14JJ2135）and the State Key Laboratory of Freshwater Ecology and Biotechnology （2012FB01 ）
文摘The grass carp(Ctenopharyngodon idella) is one of the most important cultivated fish species in China.Mounting evidences suggests that microRNAs(miRNAs) may be key regulators of skeletal muscle among the grass carp,but the knowledge of the identity of myogenic miRNAs and role of miRNAs during skeletal muscle anabolic state remains limited.In the present study,we choose 8 miRNAs previously reported to act as muscle growth-related miRNAs for fasting-refeeding research.We investigated postprandial changes in the expression of 8 miRNAs following a single satiating meal in grass carp juveniles who had been fasting for one week and found that 7 miRNAs were sharply up-regulated within 1 or 3 h after refeeding,suggesting that they may be promising candidate miRNAs involved in a fast-response signaling system that regulates fish skeletal muscle growth.
