1956-2019年乌江水文情势变化及其对鱼类的影响研究

水量变化导致河流生态水文情势发生改变,进而影响河流生态系统健康。为深入明晰乌江干流水文变异前后水文情势变化对鱼类生境的影响,选取武隆水文站1956-2019年逐日流量数据及2009-2015年12种漂流性鱼类产卵量资料,采用曼-肯德尔(M-K)检验法、累积距平法研究乌江干流变异节点,分析突变前、后逐日流量的变化趋势,通过生态水文指标变化范围法(IHA-RVA)综合评价乌江32组水文指标特征及改变度,基于水文情势变化下研究鱼类数量变化过程。结果表明:乌江流量突变年份为2009年,与彭水水电站的运行时间相同,变异后,多年平均日流量峰值波动于15000 m^(3)/s,而变异前流量峰值接近18000 m^(3)/s。乌江32组水文指标整体改变度为38.7%,处于中度变化范围;属于高度改变的低流量历时和次数改变度分别为74.64%、75.29%,发生时间推迟4 d;高流量历时和次数改变度分别为29.79%、37.83%,发生时间提前21 d。降雨与流量呈极强正相关,降雨量的逐年减少对流量有重要影响。此外,水文情势的变化易受彭水水电站的影响。流量减少和变化下的水文情势对鱼类的影响是一个累积的过程,2009年后12种漂流性卵鱼类的产卵总量大幅减少,尤以2011年最为突出,仅为1541.69×104粒。本研究可为研判乌江水文情势变化过程及制定水生态修复措施提供参考。

太湖鱼类区系变化和渔获物分析

20世纪80年代之前,太湖有鱼类107种、25科,属4个生态类型:洄游型、江湖洄游型、河流型和湖沼型.自2002年9月至2006年1月进行了太湖鱼类采集,共采得鱼类60种、18科.其中,除放流的鱼类外,都能在封闭的湖泊环境中完成生命周期(湖沼生态型);团头鲂、鲮、银鲫和尼罗罗非鱼属移植种类.团头鲂、鳙、鲢、草鱼和青鱼是从20世纪60年代起每年向湖中放流的鱼类;鳗鲡则是自1976年起放流从长江采捕的鳗苗.20世纪50年末至1985年,长江和太湖之间人为隔断,不能在静水中繁殖的鱼类在湖中消失,自然鱼类资源下降;加上过度捕捞和对繁殖群体缺少保护,导致鱼类群体小型化.最后,对太湖渔业资源的优化进行了讨论,并进一步提出对策.

Spatial and temporal variation of fish assemblages in a subtropical small stream of the Huangshan Mountain

Spatial and temporal variation of fish assemblages were investigated seasonally from May 2007 to February 2008 across 11 study sites in a subtropical small stream, the Puxi Stream, of the Huangshan Mountain. Along the longitudinal gradient from headwater to downstream, fish species richness and abundance increased gradually, but then decreased significantly at the lower reaches. The highest species richness and abundance were observed in August and the lowest in February. Based on analysis of similarities （ANOSIM）, fish assemblages were significantly different in spatial variation but not in temporal variation. Although differences were observed both among sites and among stream orders, the lower R value in order-variation suggested stream order was not the optimal factor explaining the spatial variation of fish assemblages. In addition, dam construction did not significantly alter fish assemblages in the sites adjacent to and immediately downstream to dams. Using cluster analysis and non-metric Multi Dimensional Scaling analysis （NMS）, assemblages were separated into three groups at a Bray-Curtis similarity value of 42%： the upper, middle and lower groups. Following analysis of similarity percentages of species contributions （SIM- PER）, shifts in occurrence or abundance of S. curriculus, Z. platypus, R. bitterling and A. fasciatus contributed most to the differences amongst the three groups. Standard Deviation Redundancy Analysis （RDA） suggested that habitat structure （such as elevation, substrate, and flow velocity） contributed to the spatial and temporal pattem of fish assemblages in the Puxi Stream. In conclusion, the fish assemblages in Puxi Stream presented significant spatial but not temporal variation. Human disturbance has perhaps induced the decrease in species diversity in the lower reaches. However, no significant change was observed for fish assemblages in sites far from and immediately downstream from low-head dams [Current Zoology 56 （6）： 670-677, 2010].

Spatial and seasonal variations in the trophic spectrum of demersal fish assemblages in Jiaozhou Bay, China

Trophic structure of fish communities is fundamental for ecosystem-based fisheries management, and trophic spectrum classifies fishes by their positions in food web, which provides a simple summary on the trophic structure and ecosystem function. In this study, both fish biomass and abundance trophic spectra were constructed to study the spatial and seasonal variations in the trophic structure of demersal fish assemblages in Jiaozhou Bay, China. Data were collected from four seasonal bottom trawl surveys in Jiaozhou Bay from February to November in 2011. Trophic levels(TLs) of fishes were determined by nitrogen stable isotope analysis. This study indicated that most of these trophic spectra had a single peak at trophic level(TL) of 3.4–3.7, suggesting that demersal fish assemblages of Jiaozhou Bay were dominated by secondary consumers(eg. Pholis fangi and Amblychaeturichthys hexanema). The spatial and seasonal variations of trophic spectra of Jiaozhou Bay reflected the influence of fish reproduction, fishing pressure and migration of fishes. Two-way analysis of variance(ANOVA) showed that seasonal variations in trophic spectra in Jiaozhou Bay were significant(P <0.05), but variations among different areas were not significant( P >0.05). The trophic spectrum has been proved to be a useful tool to monitor the trophic structure of fish assemblages. This study highlighted the comprehensive application of fish biomass and abundance trophic spectra in the study on trophic structure of fish assemblages.

Spatio-seasonal patterns of fish diversity,Haizhou Bay,China

Spatial-seasonal patterns in fish diversity in Haizhou Bay were studied based on stratified random surveys conducted in 2011.Principal component analysis was conducted to distinguish different diversity components,and the relationships among 11 diversity indices were explored.Generalized additive models were constructed to examine the environmental effects on diversity indices.Eleven diversity indices were grouped into four components:(1) species numbers and richness,(2) heterogeneous indices,(3) evenness,and(4) taxonomic relatedness.The results show that diversity indices among different components are complementary.Spatial patterns show that fish diversity was higher in coastal areas,which was affected by complex bottom topography and spatial variations of water mass and currents.Seasonal trends could be best explained by the seasonal migration of dominant fish species.Fish diversity generally declined with increasing depth except for taxonomic distinctness,which increased with latitude.In addition,bottom temperature had a significant effect on diversity index of richness.These results indicate that substrate complexity and environmental gradients had important influences on fish diversity patterns,and these factors should be considered in fishery resource management and conservation.Furthermore,diversity in two functional groups(demersal/pelagic fishes) was influenced by different environmental factors.Therefore,the distribution of individual species or new indicators in diversity should be applied to examine spatio-seasonal variations in fish diversity.

Seasonal variation in community structure and body length of dominant copepods around artificial reefs in Xiaoshi Island,China

This study aims to investigate the seasonal variations in copepod community structure and prosome length of dominant species from March 2009 to January 2010 around artificial reefs in Xiaoshi Island, Yellow Sea, Weihai, China. Samples were collected using two types of plankton net （Model I and Model II） for different-sized copepods. The number of taxon was calculated from the data of both the net types, while the copepod abundance was done using the samples from Model II only. Sixteen species of planktonic copepods, including 5 dominant species, were recorded. Results reveal that Oithona similis was the first dominant species from March to June, and was replaced by Paracalanus parvus in September; both dominated the copepod community in January. Acartia hongi was the second dominant species from March to September. Centropages abdominalis was the third dominant species from March to June, and was replaced by O. sirnilis in September and Corycaeus aJfinis in January. C. affinis was the fourth dominant species in September. Population density of the dominant copepods was compared with that of other similar regions. We found that the dominant species were mostly small copepods （〈1 mm） except for adult Centrapages abdominalis. Seasonal variation in prosome length of O. similis, C. abdominalis, and C. affinis, and their copepodites were studied for the first time in China. For P. parvus and A. hongi, seasonal trends in prosome length variation were similar with those in Jiaozhou Bay, Yellow Sea, Qingdao, China, in a similar temperate domain. The results are helpful for future calculation of copepod biomass and production, and for investigation of the relationship between copepods and fish resources.

Ontogenetic changes in isotopic signatures of an omnivorous fish Cultrichthys erythropterus in East Lake Taihu,China

The relationship between body size and stable isotopic signatures of the omnivorous Redfin Culter(C ultrichthys erythropterus),commonly found in East Lake Taihu,was investigated. Previous analyses of C. erythropterus stomach contents have shown that this species undergoes a diet switch from being predominantly zooplanktivorous to piscivorous during its life history. This was confirmed by stable carbon isotopic signature(δ 13 C) in this study,in which δ 13 C was positively correlated with both standard length and weight. The importance of littoral-benthic resources in supporting C. erythropterus during its lifespan was also demonstrated using a two-source mixing model,the results of which showed a significant increasing trend in the contribution of littoral-benthic energy. However,the stable nitrogen isotopic signature(δ 15N) exhibited an unusual pattern compared with previous studies. The δ 15 N of C. erythropterus showed no relationship with body size,even though dietary changes were observed. This indicated that δ 15 N alone cannot fully reflect a diet shift in a species and possible variability in isotopic signatures over its life history. This should be considered when using stable isotopic signatures to investigate intra-specific variations and the timing of life-history events,such as estimating the trophic positions of fish species.

Seasonal Variations in Serological Profiles and Growth Status of Farmed and Wild Clarias gariepinus （Burchell, 1822） Obtained from Asaba, Nigeria

Seasonal variations in serological profiles and growth status of farmed and wild Clarias gariepinus were investigated. Serological profiles of Clarias gariepinus can be an effective tool for proper monitoring of stress induced by management practices in intensive fish culture, which may elicit devastating effect on fish. Spectrophotometry procedures were used to analyze serum parameters of Clarias gariepinus. No different seasonal patterns were observed for mean values of albumin, total protein, urea, cholesterol, glucose and alanine amino-transferase. However, aspartate amino-transferase showed different seasonal pattern. Levels of cholesterol and alanine amino-transferase were highly significant in farmed and wild fish, separately. Juvenile fish had high level of urea irrespective of season. Seasonal variations in water quality parameters were observed except for pH which had no seasonal pattern. The growth exponential shows b-values between -0.048 and 7.434 for Clarias gariepinus. Adult female and juvenile fish from the wild had the highest b-value and the least b-value, separately. In this study, b-values were higher in the wild fish than the farmed fish. The condition factor for Clarias gariepinus ranged from 0.422 to 0.698, and was observed to be high in juvenile fish. With a condition factor less than 1, fish may not be doing well, probably due to environmental stress. Some serological parameters varied according to season and environment of fish. Thus, serological profile of fish is an effective and sensitive tool to monitor fish response to stress factors in the environment.

Molecular variation and evolution of the tyrosine kinase domains of insulin receptor IRa and IRb genes in Cyprinidae

The insulin receptor （IR） gene plays an important role in regulating cell growth, differentiation and development. In the present study, DNA sequences of insulin receptor genes, IRa and IRb, were amplified and sequenced from 37 representative species of the Cyprinidae and from five outgroup species from non-cyprinid Cypriniformes. Based on coding sequences （CDS） of tyro- sine kinase regions of IRa and IRb, molecular evolution and phylogenetic relationships were analyzed to better understand the characteristics of IR gene divergence in the family Cyprinidae. 1Ra and IRb were clustered into one lineage in the gene tree of the IR gene family, reconstructed using the unweighted pair group method with arithmetic mean （UPGMA）. IRa and IRb have evolved into distinct genes after IR gene duplication in Cyprinidae. For each gene, molecular evolution analyses showed that there was no significant difference among different groups in the reconstructed maximum parsimony （MP） tree of Cyprinidae; IRa and 1Rb have been subjected to similar evolutionary pressure among different lineages. Although the amino acid sequences of IRa and IRb tyrosine kinase regions were highly conserved, our analyses showed that there were clear sequence variations between the tyrosine kinase regions of IRa and IRb proteins. This indicates that IRa and IRb proteins might play different roles in the insulin signaling pathway.