对青海湖裸鲤(♀)×花斑裸鲤(♂)杂交卵的受精情况和胚胎发育过程进行观察,探索了二者杂交的可能,并介绍杂交胚胎发育过程。结果表明,青海湖裸鲤卵子和花斑裸鲤精子能完成受精,水温15~17℃时受精率为92.5%。受精卵淡黄色,微黏,沉性...对青海湖裸鲤(♀)×花斑裸鲤(♂)杂交卵的受精情况和胚胎发育过程进行观察,探索了二者杂交的可能,并介绍杂交胚胎发育过程。结果表明,青海湖裸鲤卵子和花斑裸鲤精子能完成受精,水温15~17℃时受精率为92.5%。受精卵淡黄色,微黏,沉性。受精卵卵径1.9~2.1 mm,受精2~3 min后出现黏性,开始吸水后膨胀,吸水膨胀后卵径3.5~4.1 mm。杂交胚胎发育过程分为受精、卵裂、囊胚、原肠胚、神经胚、器官形成和孵化7个时期,受精3 h 20 min后进入卵裂期,16 h 30 min后进入囊胚期,30 h 20 min后进入原肠期,39 h后进入神经胚期,42 h 50 min后进入器官形成期,100 h 50 min后进入孵化期。在水温为15~17℃下,受精卵历时136 h 30 min后全部完成出膜,胚胎畸形率1.67%。因此,青海湖裸鲤(♀)与花斑裸鲤(♂)能够进行杂交,杂交卵受精率、孵化率及畸形率与母本自交卵无显著差异(P>0.05)。展开更多
For production enhancement and procedure upgrade, the developmental phases of labora-tory-reared eggs of catfish Pelteobagrus fulvidraco were investigated. Twenty mature females and 10 males were collected from Dadong...For production enhancement and procedure upgrade, the developmental phases of labora-tory-reared eggs of catfish Pelteobagrus fulvidraco were investigated. Twenty mature females and 10 males were collected from Dadongmen wholesale fisheries market in Wuhan City on May 8, 2003. Zy-gotes were stripped from mature fish after hormone-induced ovulation, fertilized, and incubated through whole embryonic development. The fertilized eggs were stocked in density of 100 eggs/L in white square tanks of 10 L. Incubation water was dechlorinated tap water with continuous aeration. The tanks were lit directly with 60 W fluorescent bulbs with a 12 light:12 dark photoperiod. Water temperature, dissolved oxygen and pH were 29.0±0.5℃, 6.7±0.4 mg/L and 7.4±0.2, respectively. The results showed that the eggs of P. fulvidraco were yellow, sticky and contained much yolk. The mean diameter of fertilized eggs was 2.03 mm. At the water temperature of 29.0±0.5℃, the ontogenesis spent about 33 h after fertilization. From fertilization to hatching, the embryonic development can be divided into 30–40 phases, which var-ies in the emphasis and direction of development. The detailed embryonic movement was also described.展开更多
文摘对青海湖裸鲤(♀)×花斑裸鲤(♂)杂交卵的受精情况和胚胎发育过程进行观察,探索了二者杂交的可能,并介绍杂交胚胎发育过程。结果表明,青海湖裸鲤卵子和花斑裸鲤精子能完成受精,水温15~17℃时受精率为92.5%。受精卵淡黄色,微黏,沉性。受精卵卵径1.9~2.1 mm,受精2~3 min后出现黏性,开始吸水后膨胀,吸水膨胀后卵径3.5~4.1 mm。杂交胚胎发育过程分为受精、卵裂、囊胚、原肠胚、神经胚、器官形成和孵化7个时期,受精3 h 20 min后进入卵裂期,16 h 30 min后进入囊胚期,30 h 20 min后进入原肠期,39 h后进入神经胚期,42 h 50 min后进入器官形成期,100 h 50 min后进入孵化期。在水温为15~17℃下,受精卵历时136 h 30 min后全部完成出膜,胚胎畸形率1.67%。因此,青海湖裸鲤(♀)与花斑裸鲤(♂)能够进行杂交,杂交卵受精率、孵化率及畸形率与母本自交卵无显著差异(P>0.05)。
基金Supported by Scientific and Technological Bureau of Wuhan City, Hubei Province (No: 20012009105-4)
文摘For production enhancement and procedure upgrade, the developmental phases of labora-tory-reared eggs of catfish Pelteobagrus fulvidraco were investigated. Twenty mature females and 10 males were collected from Dadongmen wholesale fisheries market in Wuhan City on May 8, 2003. Zy-gotes were stripped from mature fish after hormone-induced ovulation, fertilized, and incubated through whole embryonic development. The fertilized eggs were stocked in density of 100 eggs/L in white square tanks of 10 L. Incubation water was dechlorinated tap water with continuous aeration. The tanks were lit directly with 60 W fluorescent bulbs with a 12 light:12 dark photoperiod. Water temperature, dissolved oxygen and pH were 29.0±0.5℃, 6.7±0.4 mg/L and 7.4±0.2, respectively. The results showed that the eggs of P. fulvidraco were yellow, sticky and contained much yolk. The mean diameter of fertilized eggs was 2.03 mm. At the water temperature of 29.0±0.5℃, the ontogenesis spent about 33 h after fertilization. From fertilization to hatching, the embryonic development can be divided into 30–40 phases, which var-ies in the emphasis and direction of development. The detailed embryonic movement was also described.
