This study examined the effects of dietary α-tocopheryl acetate supplementation on antioxidant enzyme activities and fillet quality in commercial-size Sparus macrocephalus. Three hundred fish [main initial weight (3...This study examined the effects of dietary α-tocopheryl acetate supplementation on antioxidant enzyme activities and fillet quality in commercial-size Sparus macrocephalus. Three hundred fish [main initial weight (350±12) g] were divided into three groups (E250, E500 and El000) and reared in 9 cages. The fish were fed for 8 weeks with three diets containing different levels of dietary α-tocopheryl acetate (289, 553, 1069 mg/kg). Over the experimental period, fish were fed to satiation and reached a final mean weight of (465±28) g without significant body weight difference and proximate composition difference. Fillet α-tocopherol was significantly (P〈0.05) different between groups, reaching levels of 14.2, 22.1, 30.9 pg/mg fillet for groups E250, E500 and El000, respectively. Total serum superoxide dismutase (SOD) activity increased significantly (P〈0.05) in fish fed the diets high in α-tocopheryl acetate, but serum glutathione peroxidase (GPX) activity was unaffected. In storage on ice, fillets of fish fed the diets high in α-tocopheryl acetate exhibited significantly lower (P〈0.05) levels of oxidation. These results suggested that increased dietary α-tocopheryl acetate could increase its flesh deposition, increase the activity of SOD and prevent lipid peroxidation ofSparus macrocephalus fillets in retail storage on ice.展开更多
We evaluated the effect of ration level (RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok (initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration level...We evaluated the effect of ration level (RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok (initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration levels: starvation, 2%, 3%, 4% bwd (body weight per day, based on initial mean values), and apparent satiation. Feed consumption, apparent digestibility, and growth were directly measured. Specific growth rates in terms of wet weight, dry weight, protein, and energy increased logarithmically with an increase in ration levels. The relationship between specific growth rate in terms of wet weight (SGRw, %/d) and RL (%) was characterized by a decelerating curve: SGRw=- 1.417+3.1661n(RL+ 1). The apparent digestibility coefficients of energy exhibited a decreasing pattern with increasing ration level, and there was a significant difference among different RLs. Body composition was significantly affected by ration size. The relationship between feed efficiency rate in terms of energy (FERe) and RL was: FERe=- 14.167+23.793RL-3.367(RL)2, and the maximum FERe was observed at a 3.53% ration. The maintenance requirement for energy of juvenile lenok was 105.39 kJ BW (kg)-0.80/d, the utilization efficiency of DE for growth was 0.496. The energy budget equation at satiation was: 100IE=29.03FE+5.78(ZE+UE)+39.56 HE+25.63 RE, where IE is feed energy, FE is fecal energy, ZE+UE is excretory energy, HE is heat production, and RE is recovered energy. Our results suggest that the most suitable feeding rate for juvenile lenok aquaculture for wet weight growth is 2.89% bwd, whereas for energy growth, the suggested rate is 3.53% bwd at this growth stage.展开更多
We evaluated the effects of supplemental coated and crystalline methionine (Met) on the growth performance and feed utilization of juvenile cobia (Rachycentron canadum Linnaeus) in a 60-d feeding trial. Fish group...We evaluated the effects of supplemental coated and crystalline methionine (Met) on the growth performance and feed utilization of juvenile cobia (Rachycentron canadum Linnaeus) in a 60-d feeding trial. Fish groups were fed one of six isonitrogenous and isolipidic diets: 1) fishmeal control; 2) un- supplemented experimental (low-fish-meal diet deficient in Met); or 3) one of four Met diets supplemented with crystalline L-Met, cellulose-acetate-phthalate coated L-Met, acrylic-resin coated L-Met, or tripalmitin- polyvinyl alcohol coated L-Met. The test diets were fed to triplicate groups of cobia (initial body weight 5.40-4-0.07 g) twice a day. The weight gain and specific growth rate of the fish fed the RES diet were highest among the Met-supplemented groups and were 23.64% and 7.99%, respectively, higher than those of the fish fed with the un-supplemented experimental diet (P〈0.05). The protein efficiency ratio of the fish fed the MET diet was significantly higher than that of the fish fed the un-supplemented experimental diet and the fish in the other methionine supplementation groups (P〈0.05). Our results suggest that supplementation of crystalline Met in low-fish-meal diets promotes the growth performance of juvenile cobia.展开更多
An 8-week feeding trial was conducted to assess the effect of soybean meal (SBM), groundnut meal (GNM) and blood meal (BM) addition in diets with or without (1%) fishmeal on the performance, N-digestibility an...An 8-week feeding trial was conducted to assess the effect of soybean meal (SBM), groundnut meal (GNM) and blood meal (BM) addition in diets with or without (1%) fishmeal on the performance, N-digestibility and cost of production of growing grasscutters. Fifty-four grasscutters (9-week-old) of mixed sexes were randomly assigned to six dietary treatments, using a completely randomized design (CRD) in a 2 × 3 factorial arrangement, with three grasscutters per treatment and three replicates each. Treatments 1-3 (TI-T3) contained no fishmeal, whilst treatments 4-6 (T4-T6) had 1% fishmeal added. The diets were formulated to be isocaloric and isonitrogenous. N-digestibility was significantly high for SBM diets with or without fishmeal, 83.91% and 82.19%, respectively, followed by BM diets. Average daily gain (ADG) was significantly (P 〈 0.05) high (12.95 g) for BM diet without fishmeal. Feed cost per kg diet was the lowest (US$0.195) for the same BM diet. The results indicated that the inclusion of SBM and BM in the diet of grasscutters with or without 1% fishmeal could improve performance and it may be the most economical for feeding BM in the diet without fishmeal.展开更多
The effect of two diets containing fish acid silage were studied, the first one offered as dry diet and the second one as a wet diet, in final culture cycle of PactS. Previous experiences showed that diets without fis...The effect of two diets containing fish acid silage were studied, the first one offered as dry diet and the second one as a wet diet, in final culture cycle of PactS. Previous experiences showed that diets without fishmeal maintain same growth rate in semi intensive system, compared to diets containing 8% and 20% fish meal. Feeding trials were carried out in CENADAC (27°32′ S, 58030' W) in a semi intensive pond culture system for 122 days. Two experimental feed including fish silage, a liquid product obtained of fish residues treated by enzymes action, were formulated. Fish silage was prepared by mincing and blending fish viscera with sufficient formic acid (2.11% v/w) to attain a stable pH of 3.5. Fish were daily fed initially with the dry ration at 1.3% bw/d and at a rate of 0.55 g of protein/100 g of fish with the wet ration. At the end of the experience the average weight observed for dry fed fish was 1,513.5 g and 1,560.3 g for those wet fed. The daily growth was 3.69 g/d for the first group and 3.9 g/d for the second one. The final FCR was 2.72 and 3.01 for each group without significant differences. These results showed that it is possible to grow out paco with wet self made feed without fish meal and with similar growth that those fed dry diet.展开更多
The goal of this study was to investigate the behaviour of rainbow trout(n=30),Oncorhynchus mykiss,in small raceways when either self-feeders(T2) or hand-feeding(t2) were used.The method of food delivery in T2 was def...The goal of this study was to investigate the behaviour of rainbow trout(n=30),Oncorhynchus mykiss,in small raceways when either self-feeders(T2) or hand-feeding(t2) were used.The method of food delivery in T2 was defensible while that of t2 was indefensible.Fish in both raceways were subjected to restricted feeding(RF) for 25 days.Food was available in the morning(09:00-10:00) in the downstream area and in the afternoon(16:00-17:00) in the upstream area of the raceways.The results showed that the behaviour of rainbow trout was significantly different under interference competition(T2) for food compared with that under scramble competition(t2).RF in T2 fish limited food availability to meal times when feeding rewards were available while t2 fish only responded to the location of food delivery.The aggressive fish in T2 were dominant,and t2 fish at high densities showed intense social interactions under the indefensible pattern of food distribution;these interactions did not dampen to a minimum level to suppress the development of dominance hierarchies.Further,the stocking density did not break down the dominance hierarchies between the T2 fish.This suggests that decreased efficiency in the search for food or inefficient foraging,induced by interference competition at high densities,affected the behaviour of rainbow trout.展开更多
The dietary protein requirement of juvenile turbot(initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with ...The dietary protein requirement of juvenile turbot(initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with fish meal as protein source, which contained different concentrations of protein(47.2%, 51.0%, 54.6%, 59.3% and 63.6% of dry diet). Three groups of fish with 18 individuals in each, were cultured in 300-L tanks and fed twice a day for 8 weeks. During culture, temperature was controlled between 15.0 and 18.0℃, salinity was controlled between 28.5 and 32.0, acidity was controlled between p H7.8 and p H8.5, and ammonia nitrogen was maintained below 0.03 mg L-1 and dissolved oxygen was maintained about 7 mg L-1. Results showed that the growth of fish was significantly affected by dietary protein content(P < 0.05). Specific growth rate(SGR) of turbot increased when dietary protein content varied between 47.2% and 51.0%(P < 0.05), and then kept stable when dietary protein content was higher than 51.0%. Fish which were fed the diet containing 63.6% protein showed the highest SGR while those fed the diet containing 59.3% protein showed the highest feed efficiency rate. No significant difference of feed intake and protein efficiency ratio was found among experimental diets(P > 0.05). Broken-line regression analysis of SGR showed that the optimal dietary protein requirement of turbot was about 57.0%.展开更多
Poverty, inadequate dietary intake, and diseases are the major causes of malnutrition in Nigeria. Averagely, Nigerians consume about 5.5 g of animal protein per day which is low compared to the minimum of 30 g per per...Poverty, inadequate dietary intake, and diseases are the major causes of malnutrition in Nigeria. Averagely, Nigerians consume about 5.5 g of animal protein per day which is low compared to the minimum of 30 g per person per day as recommended by Food and Agricultural Organization. The concept of household food security ensures that adequate food can be obtained, either through home production or purchases. Snail production is one of the means through which these ills could be eliminated. Protein deficiency that is endemic in developing nations can be reduced through the domestication of micro-livestock like snail which is rich in protein and iron. Sixty Achatina achatina hatchlings were used in a 90 days feeding trial. The hatchlings were assigned to four treatments in a Completely Randomized Design with three replicates each. Maggot meal was incorporated at the levels of 0.6, 1.4, and 2 kg per 100 kg of feed in treatments 2, 3 and 4, respectively. Treatment 1 had no maggot meal and served as the control. Results showed that snail weight, shell length and shell width increased with increase in the levels of maggot meal. Hatchlings on T3 and T4 had statistically similar values (P 〉 0.01) which were significantly (P 〈 0.01) higher than values obtained for hatchlings on T1 and T2 .Weight gain and feed conversion values of hatchlings on T3 and T4 were also significantly higher than values observed for hatchlings on T1 and T2. Therefore, maggot meal could effectively replace fishmeal in the diet of African giant snail hatchlings.展开更多
基金Project (No. 2006c12098) supported by the Science and TechnologyDepartment of Zhejiang Province, China
文摘This study examined the effects of dietary α-tocopheryl acetate supplementation on antioxidant enzyme activities and fillet quality in commercial-size Sparus macrocephalus. Three hundred fish [main initial weight (350±12) g] were divided into three groups (E250, E500 and El000) and reared in 9 cages. The fish were fed for 8 weeks with three diets containing different levels of dietary α-tocopheryl acetate (289, 553, 1069 mg/kg). Over the experimental period, fish were fed to satiation and reached a final mean weight of (465±28) g without significant body weight difference and proximate composition difference. Fillet α-tocopherol was significantly (P〈0.05) different between groups, reaching levels of 14.2, 22.1, 30.9 pg/mg fillet for groups E250, E500 and El000, respectively. Total serum superoxide dismutase (SOD) activity increased significantly (P〈0.05) in fish fed the diets high in α-tocopheryl acetate, but serum glutathione peroxidase (GPX) activity was unaffected. In storage on ice, fillets of fish fed the diets high in α-tocopheryl acetate exhibited significantly lower (P〈0.05) levels of oxidation. These results suggested that increased dietary α-tocopheryl acetate could increase its flesh deposition, increase the activity of SOD and prevent lipid peroxidation ofSparus macrocephalus fillets in retail storage on ice.
基金Supported by the Special Fund for Agro-Scientific Research in the Public Interest of China(No.201003055)the National Key Technology Research and Development Program of China(Nos.2012BAD25B10,2012BAD26B05)the Central-Level Non-Profit Scientific Research Institutes Special Funds of China(No.HSY201412)
文摘We evaluated the effect of ration level (RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok (initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration levels: starvation, 2%, 3%, 4% bwd (body weight per day, based on initial mean values), and apparent satiation. Feed consumption, apparent digestibility, and growth were directly measured. Specific growth rates in terms of wet weight, dry weight, protein, and energy increased logarithmically with an increase in ration levels. The relationship between specific growth rate in terms of wet weight (SGRw, %/d) and RL (%) was characterized by a decelerating curve: SGRw=- 1.417+3.1661n(RL+ 1). The apparent digestibility coefficients of energy exhibited a decreasing pattern with increasing ration level, and there was a significant difference among different RLs. Body composition was significantly affected by ration size. The relationship between feed efficiency rate in terms of energy (FERe) and RL was: FERe=- 14.167+23.793RL-3.367(RL)2, and the maximum FERe was observed at a 3.53% ration. The maintenance requirement for energy of juvenile lenok was 105.39 kJ BW (kg)-0.80/d, the utilization efficiency of DE for growth was 0.496. The energy budget equation at satiation was: 100IE=29.03FE+5.78(ZE+UE)+39.56 HE+25.63 RE, where IE is feed energy, FE is fecal energy, ZE+UE is excretory energy, HE is heat production, and RE is recovered energy. Our results suggest that the most suitable feeding rate for juvenile lenok aquaculture for wet weight growth is 2.89% bwd, whereas for energy growth, the suggested rate is 3.53% bwd at this growth stage.
基金Supported by the Special Fund for Agro-Scientific Research in the Public Interest(No.201003020)the Pearl River Scholar Funded Scheme for Universities and Colleges in Guangdong Province(GDUPS,No.2011)the Natural Science Foundation of Guangdong Ocean University of China(No.1212345)
文摘We evaluated the effects of supplemental coated and crystalline methionine (Met) on the growth performance and feed utilization of juvenile cobia (Rachycentron canadum Linnaeus) in a 60-d feeding trial. Fish groups were fed one of six isonitrogenous and isolipidic diets: 1) fishmeal control; 2) un- supplemented experimental (low-fish-meal diet deficient in Met); or 3) one of four Met diets supplemented with crystalline L-Met, cellulose-acetate-phthalate coated L-Met, acrylic-resin coated L-Met, or tripalmitin- polyvinyl alcohol coated L-Met. The test diets were fed to triplicate groups of cobia (initial body weight 5.40-4-0.07 g) twice a day. The weight gain and specific growth rate of the fish fed the RES diet were highest among the Met-supplemented groups and were 23.64% and 7.99%, respectively, higher than those of the fish fed with the un-supplemented experimental diet (P〈0.05). The protein efficiency ratio of the fish fed the MET diet was significantly higher than that of the fish fed the un-supplemented experimental diet and the fish in the other methionine supplementation groups (P〈0.05). Our results suggest that supplementation of crystalline Met in low-fish-meal diets promotes the growth performance of juvenile cobia.
文摘An 8-week feeding trial was conducted to assess the effect of soybean meal (SBM), groundnut meal (GNM) and blood meal (BM) addition in diets with or without (1%) fishmeal on the performance, N-digestibility and cost of production of growing grasscutters. Fifty-four grasscutters (9-week-old) of mixed sexes were randomly assigned to six dietary treatments, using a completely randomized design (CRD) in a 2 × 3 factorial arrangement, with three grasscutters per treatment and three replicates each. Treatments 1-3 (TI-T3) contained no fishmeal, whilst treatments 4-6 (T4-T6) had 1% fishmeal added. The diets were formulated to be isocaloric and isonitrogenous. N-digestibility was significantly high for SBM diets with or without fishmeal, 83.91% and 82.19%, respectively, followed by BM diets. Average daily gain (ADG) was significantly (P 〈 0.05) high (12.95 g) for BM diet without fishmeal. Feed cost per kg diet was the lowest (US$0.195) for the same BM diet. The results indicated that the inclusion of SBM and BM in the diet of grasscutters with or without 1% fishmeal could improve performance and it may be the most economical for feeding BM in the diet without fishmeal.
文摘The effect of two diets containing fish acid silage were studied, the first one offered as dry diet and the second one as a wet diet, in final culture cycle of PactS. Previous experiences showed that diets without fishmeal maintain same growth rate in semi intensive system, compared to diets containing 8% and 20% fish meal. Feeding trials were carried out in CENADAC (27°32′ S, 58030' W) in a semi intensive pond culture system for 122 days. Two experimental feed including fish silage, a liquid product obtained of fish residues treated by enzymes action, were formulated. Fish silage was prepared by mincing and blending fish viscera with sufficient formic acid (2.11% v/w) to attain a stable pH of 3.5. Fish were daily fed initially with the dry ration at 1.3% bw/d and at a rate of 0.55 g of protein/100 g of fish with the wet ration. At the end of the experience the average weight observed for dry fed fish was 1,513.5 g and 1,560.3 g for those wet fed. The daily growth was 3.69 g/d for the first group and 3.9 g/d for the second one. The final FCR was 2.72 and 3.01 for each group without significant differences. These results showed that it is possible to grow out paco with wet self made feed without fish meal and with similar growth that those fed dry diet.
文摘The goal of this study was to investigate the behaviour of rainbow trout(n=30),Oncorhynchus mykiss,in small raceways when either self-feeders(T2) or hand-feeding(t2) were used.The method of food delivery in T2 was defensible while that of t2 was indefensible.Fish in both raceways were subjected to restricted feeding(RF) for 25 days.Food was available in the morning(09:00-10:00) in the downstream area and in the afternoon(16:00-17:00) in the upstream area of the raceways.The results showed that the behaviour of rainbow trout was significantly different under interference competition(T2) for food compared with that under scramble competition(t2).RF in T2 fish limited food availability to meal times when feeding rewards were available while t2 fish only responded to the location of food delivery.The aggressive fish in T2 were dominant,and t2 fish at high densities showed intense social interactions under the indefensible pattern of food distribution;these interactions did not dampen to a minimum level to suppress the development of dominance hierarchies.Further,the stocking density did not break down the dominance hierarchies between the T2 fish.This suggests that decreased efficiency in the search for food or inefficient foraging,induced by interference competition at high densities,affected the behaviour of rainbow trout.
基金supported by the earmarked fund for Modern Agro-Industry Technology Research System with Grant No. CARS 50-G-08
文摘The dietary protein requirement of juvenile turbot(initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with fish meal as protein source, which contained different concentrations of protein(47.2%, 51.0%, 54.6%, 59.3% and 63.6% of dry diet). Three groups of fish with 18 individuals in each, were cultured in 300-L tanks and fed twice a day for 8 weeks. During culture, temperature was controlled between 15.0 and 18.0℃, salinity was controlled between 28.5 and 32.0, acidity was controlled between p H7.8 and p H8.5, and ammonia nitrogen was maintained below 0.03 mg L-1 and dissolved oxygen was maintained about 7 mg L-1. Results showed that the growth of fish was significantly affected by dietary protein content(P < 0.05). Specific growth rate(SGR) of turbot increased when dietary protein content varied between 47.2% and 51.0%(P < 0.05), and then kept stable when dietary protein content was higher than 51.0%. Fish which were fed the diet containing 63.6% protein showed the highest SGR while those fed the diet containing 59.3% protein showed the highest feed efficiency rate. No significant difference of feed intake and protein efficiency ratio was found among experimental diets(P > 0.05). Broken-line regression analysis of SGR showed that the optimal dietary protein requirement of turbot was about 57.0%.
文摘Poverty, inadequate dietary intake, and diseases are the major causes of malnutrition in Nigeria. Averagely, Nigerians consume about 5.5 g of animal protein per day which is low compared to the minimum of 30 g per person per day as recommended by Food and Agricultural Organization. The concept of household food security ensures that adequate food can be obtained, either through home production or purchases. Snail production is one of the means through which these ills could be eliminated. Protein deficiency that is endemic in developing nations can be reduced through the domestication of micro-livestock like snail which is rich in protein and iron. Sixty Achatina achatina hatchlings were used in a 90 days feeding trial. The hatchlings were assigned to four treatments in a Completely Randomized Design with three replicates each. Maggot meal was incorporated at the levels of 0.6, 1.4, and 2 kg per 100 kg of feed in treatments 2, 3 and 4, respectively. Treatment 1 had no maggot meal and served as the control. Results showed that snail weight, shell length and shell width increased with increase in the levels of maggot meal. Hatchlings on T3 and T4 had statistically similar values (P 〉 0.01) which were significantly (P 〈 0.01) higher than values obtained for hatchlings on T1 and T2 .Weight gain and feed conversion values of hatchlings on T3 and T4 were also significantly higher than values observed for hatchlings on T1 and T2. Therefore, maggot meal could effectively replace fishmeal in the diet of African giant snail hatchlings.