The spatial distribution pattern of organisms is a basic issue in understanding the mechanisms of community assembly. Although the spatial distributions of animals and plants have been well studied,those of microorgan...The spatial distribution pattern of organisms is a basic issue in understanding the mechanisms of community assembly. Although the spatial distributions of animals and plants have been well studied,those of microorganisms are still being debated. In this study, we used a fi sh gut microecosystem to detect the spatial pattern of microbes, because it can provide a relatively unifi ed and stable environment. Results suggest that the turnover of intestinal bacterial assemblages showed a weak but highly signifi cant negative correlation between similarity and distances in the microbial community, in respect of both grass carp intestinal loci distances and the geographical distance between fi sh sampling sites. Our results also suggest that intestinal bacterial assemblages responded to differences within the external environment and within different parts of the fi sh themselves. These results show that some, or possibly all, microbes are restricted in their distribution and that environmental factors are also important infl uences on the structure of intestinal bacterial assemblages. The fi sh gut microecosystem is useful in promoting study of the spatial distribution patterns of microorganisms.展开更多
The intestinal bacteria of vertebrates form a close relationship with their host.External and internal conditions of the host,including its habitat,affect the intestinal bacterial community.Similarly,the intestinal ba...The intestinal bacteria of vertebrates form a close relationship with their host.External and internal conditions of the host,including its habitat,affect the intestinal bacterial community.Similarly,the intestinal bacterial community can,in turn,influence the host,particularly with respect to disease resistance.We compared the intestinal bacterial communities of grass carp that were collected from farm-ponds or a lake.We conducted denaturing gradient gel electrophoresis of amplified 16S rRNA genes,from which 66 different operational taxonomic units were identified.Using both the unweighted pair-group method with arithmetic means clustering and principal component analysis ordination,we found that the intestinal bacterial communities from the two groups of pond fish were clustered together and inset into the clusters of wild fish,except for DF-7,and there was no significant correlation between genetic diversity of grass carp and their intestinal bacterial communities(Mantel one-tailed test,R=0.157,P=0.175).Cetobacterium appeared more frequently in the intestine of grass carp collected from pond.A more thorough understanding of the role played by intestinal microbiota on fish health would be of considerable benefit to the aquaculture industry.展开更多
The aim of the study was to examine the bacterial community associated with the intestinal mucus of young farmed puffer fish Takifugu rubripes. Polymerase chain reaction and partial 16 S r DNA sequencing was performed...The aim of the study was to examine the bacterial community associated with the intestinal mucus of young farmed puffer fish Takifugu rubripes. Polymerase chain reaction and partial 16 S r DNA sequencing was performed on DNA from bacteria cultivated on Zobell 2216 E medium. All the isolates were classified into two phyla—Proteobacteria and Firmicutes. Proteobacteria were the dominant, culturable intestinal microbiota(68.3%). At the genus level, V ibrio, Enterobacter, Bacillus, Pseudomonas, Exiguobacterium, Staphylococcus, Acinetobacter, Pseudoalteromonas and S hewanella were isolated from the intestine, with representatives of the genera V ibrio, Enterobacter and Bacillus accounting for 70.7% of the total. This is the first report of Enterobacter, Bacillus, Exiguobacterium and Staphylococcus as part of the intestinal bacterial microflora in T. rubripes. The profile of the culturable bacterial community differed between samples collected from the same tank at 2-month intervals, as indicated by Bray-Curtis and Sorensen indices, and the impact on the intestinal physiology and health of puffer fish requires further investigation.展开更多
基金Supported by the National Basic Research Program of China(973 Program)(No.2009CB118705)the National Natural Science Foundation of China(Nos.30970358,31071896)the Youth Innovation Promotion Association,CAS
文摘The spatial distribution pattern of organisms is a basic issue in understanding the mechanisms of community assembly. Although the spatial distributions of animals and plants have been well studied,those of microorganisms are still being debated. In this study, we used a fi sh gut microecosystem to detect the spatial pattern of microbes, because it can provide a relatively unifi ed and stable environment. Results suggest that the turnover of intestinal bacterial assemblages showed a weak but highly signifi cant negative correlation between similarity and distances in the microbial community, in respect of both grass carp intestinal loci distances and the geographical distance between fi sh sampling sites. Our results also suggest that intestinal bacterial assemblages responded to differences within the external environment and within different parts of the fi sh themselves. These results show that some, or possibly all, microbes are restricted in their distribution and that environmental factors are also important infl uences on the structure of intestinal bacterial assemblages. The fi sh gut microecosystem is useful in promoting study of the spatial distribution patterns of microorganisms.
基金Supported by the National Basic Research Program of China(973 Program)(No.2009CB118705)the National Natural Science Foundation of China(No.30970358)
文摘The intestinal bacteria of vertebrates form a close relationship with their host.External and internal conditions of the host,including its habitat,affect the intestinal bacterial community.Similarly,the intestinal bacterial community can,in turn,influence the host,particularly with respect to disease resistance.We compared the intestinal bacterial communities of grass carp that were collected from farm-ponds or a lake.We conducted denaturing gradient gel electrophoresis of amplified 16S rRNA genes,from which 66 different operational taxonomic units were identified.Using both the unweighted pair-group method with arithmetic means clustering and principal component analysis ordination,we found that the intestinal bacterial communities from the two groups of pond fish were clustered together and inset into the clusters of wild fish,except for DF-7,and there was no significant correlation between genetic diversity of grass carp and their intestinal bacterial communities(Mantel one-tailed test,R=0.157,P=0.175).Cetobacterium appeared more frequently in the intestine of grass carp collected from pond.A more thorough understanding of the role played by intestinal microbiota on fish health would be of considerable benefit to the aquaculture industry.
基金Supported by the Public Science and Technology Research Funds Projects of Ocean(No.201205025)
文摘The aim of the study was to examine the bacterial community associated with the intestinal mucus of young farmed puffer fish Takifugu rubripes. Polymerase chain reaction and partial 16 S r DNA sequencing was performed on DNA from bacteria cultivated on Zobell 2216 E medium. All the isolates were classified into two phyla—Proteobacteria and Firmicutes. Proteobacteria were the dominant, culturable intestinal microbiota(68.3%). At the genus level, V ibrio, Enterobacter, Bacillus, Pseudomonas, Exiguobacterium, Staphylococcus, Acinetobacter, Pseudoalteromonas and S hewanella were isolated from the intestine, with representatives of the genera V ibrio, Enterobacter and Bacillus accounting for 70.7% of the total. This is the first report of Enterobacter, Bacillus, Exiguobacterium and Staphylococcus as part of the intestinal bacterial microflora in T. rubripes. The profile of the culturable bacterial community differed between samples collected from the same tank at 2-month intervals, as indicated by Bray-Curtis and Sorensen indices, and the impact on the intestinal physiology and health of puffer fish requires further investigation.
