The reactive precipitation process of procaine benzylpenicillin is reviewed, while such secondary processes as ageing, agglomeration, breakage, and the effects of operation parameters on crystal size are emphasized. I...The reactive precipitation process of procaine benzylpenicillin is reviewed, while such secondary processes as ageing, agglomeration, breakage, and the effects of operation parameters on crystal size are emphasized. In the reactive precipitation the ageing of particles has a little effect on the process, while the greater effect comes from the agglomeration and breakage of particles, furthermore, the mixing has also notable influence on the product size. All of these provide the bases for further study on reactive precipitation.展开更多
【目的】探究一株李维斯链霉菌的抗病促生性能,鉴定其抑菌活性成分。【方法】从耐旱稗草根际分离到一株能抑制多种病原真菌及细菌的链霉菌,采用形态学观察及16SrRNA基因序列分析的方法对菌株进行鉴定;运用液相色谱和高分辨质谱技术鉴定...【目的】探究一株李维斯链霉菌的抗病促生性能,鉴定其抑菌活性成分。【方法】从耐旱稗草根际分离到一株能抑制多种病原真菌及细菌的链霉菌,采用形态学观察及16SrRNA基因序列分析的方法对菌株进行鉴定;运用液相色谱和高分辨质谱技术鉴定活性物质成分;利用二代Illumina与三代Nanopore测序技术进行全基因组测序;antiSMASH分析该菌株中活性成分的生物合成基因簇。【结果】李维斯链霉菌L2的菌体及发酵液能高效抑制革兰氏阳性菌及多种植物病原真菌的生长,能在铬天青平板(chrome azurol sulphonate,CAS)上产生较大的噬铁圈,还具有较好的产吲哚乙酸(indole-3-acetic acid,IAA)的能力。其抑菌活性成分的m/z为537.1020[M+H]^(+)(calcd for C_(27)H_(20)O_(12),537.1035,2.2×10^(-6)),523.0863[M+H]^(+)(calcd for C_(26)H_(18)O_(12),523.0878,2.9×10^(-6)),分别与α-鲁霉素、β-鲁霉素、γ-鲁霉素的精确分子量相符,误差小于1/5000000,其高效液相色谱保留时间与鲁霉素标准品一致。该菌基因组总长8.8 Mb,预测编码有鲁霉素等32个次级代谢产物生物合成基因簇。【结论】李维斯链霉菌L2具有较好的抗病促生性能,适宜进一步开发为生防菌剂,其抑菌活性成分为鲁霉素。展开更多
文摘The reactive precipitation process of procaine benzylpenicillin is reviewed, while such secondary processes as ageing, agglomeration, breakage, and the effects of operation parameters on crystal size are emphasized. In the reactive precipitation the ageing of particles has a little effect on the process, while the greater effect comes from the agglomeration and breakage of particles, furthermore, the mixing has also notable influence on the product size. All of these provide the bases for further study on reactive precipitation.
文摘【目的】探究一株李维斯链霉菌的抗病促生性能,鉴定其抑菌活性成分。【方法】从耐旱稗草根际分离到一株能抑制多种病原真菌及细菌的链霉菌,采用形态学观察及16SrRNA基因序列分析的方法对菌株进行鉴定;运用液相色谱和高分辨质谱技术鉴定活性物质成分;利用二代Illumina与三代Nanopore测序技术进行全基因组测序;antiSMASH分析该菌株中活性成分的生物合成基因簇。【结果】李维斯链霉菌L2的菌体及发酵液能高效抑制革兰氏阳性菌及多种植物病原真菌的生长,能在铬天青平板(chrome azurol sulphonate,CAS)上产生较大的噬铁圈,还具有较好的产吲哚乙酸(indole-3-acetic acid,IAA)的能力。其抑菌活性成分的m/z为537.1020[M+H]^(+)(calcd for C_(27)H_(20)O_(12),537.1035,2.2×10^(-6)),523.0863[M+H]^(+)(calcd for C_(26)H_(18)O_(12),523.0878,2.9×10^(-6)),分别与α-鲁霉素、β-鲁霉素、γ-鲁霉素的精确分子量相符,误差小于1/5000000,其高效液相色谱保留时间与鲁霉素标准品一致。该菌基因组总长8.8 Mb,预测编码有鲁霉素等32个次级代谢产物生物合成基因簇。【结论】李维斯链霉菌L2具有较好的抗病促生性能,适宜进一步开发为生防菌剂,其抑菌活性成分为鲁霉素。
