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5种鲤形目鱼的β珠蛋白基因cDNA的克隆
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作者 常重杰 陈颖 杜启艳 《中国水产科学》 CAS CSCD 北大核心 2003年第2期102-105,共4页
根据β珠蛋白的N末端和C末端氨基酸序列的保守性设计20bp长的简并引物,用RT PCR方法扩增并克隆了鲤(Cyprinuscarpio)、草鱼(Ctenopharyngodonidellus)、鲫(CarassiusauratusLinnaeus)、泥鳅(Misgurnusanguillicaudatus)、大鳞副泥鳅(Par... 根据β珠蛋白的N末端和C末端氨基酸序列的保守性设计20bp长的简并引物,用RT PCR方法扩增并克隆了鲤(Cyprinuscarpio)、草鱼(Ctenopharyngodonidellus)、鲫(CarassiusauratusLinnaeus)、泥鳅(Misgurnusanguillicaudatus)、大鳞副泥鳅(Paramisgurnusdabryanus)5种鲤形目鱼的β珠蛋白基因cDNA。结果表明,克隆的5种鲤形目鱼的β珠蛋白基因cDNA全长为441bp。但它们所编码的氨基酸序列却有较大的差异,鲤和泥鳅的序列相似性最高,达97.93%,而泥鳅和大鳞副泥鳅的相似性最小,仅有80.68%,其余的相似性介于二者之间。 展开更多
关键词 鲤形目鱼类 Β珠蛋白 基因CDNA 克隆 氨基酸序列 RT—PCR 转基因 抗冻蛋白基因 水中溶氧量
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Taxonomical notes on selected freshwater fish species described from northern and central Vietnam (Cypriniformes: Balitoridae, Cobitidae, Cyprinidae, Nemacheilidae; Perciformes: Channidae, Osphronemidae; Synbranchiformes: Mastacembelidae) 被引量:2
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作者 Marco Endruweit 《Zoological Research》 CAS CSCD 北大核心 2014年第2期142-159,共18页
Selected,little known taxa of northern and central Vietnamese freshwater fish species are reviewed.Nomenclatural acts are taken:Hemibarbus lehoai is placed in synonymy of H.maculatus,Paracobitis hagiangensis in synony... Selected,little known taxa of northern and central Vietnamese freshwater fish species are reviewed.Nomenclatural acts are taken:Hemibarbus lehoai is placed in synonymy of H.maculatus,Paracobitis hagiangensis in synonymy of Schistura caudofurca.A neotype of Micronemacheilus bacmeensis is assigned.The name Channa hanamensis is treated as a nomen nudum.Two labeonine species described from China are nomenclaturally affected:Garra findolabium is transferred to Vinagarra and its specific epithet is treated as a noun in apposition;the specific epithet of Sinigarra napoense is corrected to napoensis. 展开更多
关键词 New species Ichthyology Taxonomy Nomenclature VIETNAM
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Four new records of fish species (Cypriniformes: Nemacheilidae, Balitoridae; Characiformes: Prochilodontidae) and corrections of two misidentified fish species (Tetraodontiformes: Tetraodontidae; Beloniformes: Belonidae) in Yunnan, China
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作者 Marco Endruweit 《Zoological Research》 CAS CSCD 北大核心 2014年第1期51-58,共8页
In this study, six fish species of five families are reported for the first time from Yunnan Province, China. The nemacheilid Schistura arnplizona Kottelat, 2000 is reported from the Luosuojiang River and Nanlahe Rive... In this study, six fish species of five families are reported for the first time from Yunnan Province, China. The nemacheilid Schistura arnplizona Kottelat, 2000 is reported from the Luosuojiang River and Nanlahe River subbasins, Mekong basin; the prochilodontid Prochilodus lineatus (Valenciennes, 1837), the balitorid Vanmanenia serrilineata Kottelat, 2000, and the tetraodontid Monotrete turgidus Kottelat, 2000, from Nanlahe River subbasin, Mekong basin; the balitorid Beaz^fortia daon (Mai, 1978), and the belonid Xenentodon canciloides (Bleeker, 1854), both, from Black River subbasin, Red River basin. The freshwater puffer M. turgidus and the needlefish ,E canciloides have been previously misidentified as Tettraodon leiurus (Bleeker, 1950) and Tylosurus strongylurus (van Hasselt, 1823), respectively. 展开更多
关键词 New record MISIDENTIFICATION Mekong basin Red River YUNNAN
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Phylogeny of the Labeoninae (Teleostei, Cypriniformes) based on nuclear DNA sequences and implications on character evolution and biogeography 被引量:2
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作者 Lanping ZHENG Junxing YANG Xiaoyong CHEN 《Current Zoology》 SCIE CAS CSCD 2012年第6期837-850,共14页
Abstract The Labeoninae is a subfamily of the family Cyprinidae, Order Cypriniformes. Oromandibular morphology within the Labeoninae is the greatest among cyprinid fishes. Although several phylogenetic studies about l... Abstract The Labeoninae is a subfamily of the family Cyprinidae, Order Cypriniformes. Oromandibular morphology within the Labeoninae is the greatest among cyprinid fishes. Although several phylogenetic studies about labeonines have been undertaken the results have been inconsistent and a comprehensive phylogeny is needed. Further, an incongruence between morphological and molecular phylogeny requires a systematic exploration of the significance of morphological characters on the basis of the molecular phylogeny. In this study, a total of 292 nucleotide sequences from 73 individuals (representing 24 genera and 73 species) of Labeoninae were analyzed. The results of the phylogenetic analysis indicate that there are four major clades within Labeoninae and three monophyletic lineages within the fourth clade. Results of the character evolution show that all oromandibular morphological characters are homoplastically distributed on the molecular phylogenetic tree and suggests that these characters evolved several times during the history of labeonines. In particular, the labeonine, a specific disc on the lower lip, has been acquired three times and reversed twice. These morphological characters do not have systematic significance but can be useful for taxonomy. The results of biogeography suggest that the Labeoninae originated from Southeast Asia and separately dispersed to Africa, East Asia and South Asia. 展开更多
关键词 PHYLOGENY Character evolution BIOGEOGRAPHY LABEONINAE
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