应用过氧化物酶抗过氧化物酶(PAP)免疫组织化学技术,用4种哺乳动物中培育出的抗血清对草鱼、青鱼、鲤和翘嘴红鲌4种鲤科鱼的肠内分泌细胞进行免疫、组织化学的鉴别和定位.证实了高血糖素免疫活性内分泌细胞在草鱼整个肠道中均呈阳性反应...应用过氧化物酶抗过氧化物酶(PAP)免疫组织化学技术,用4种哺乳动物中培育出的抗血清对草鱼、青鱼、鲤和翘嘴红鲌4种鲤科鱼的肠内分泌细胞进行免疫、组织化学的鉴别和定位.证实了高血糖素免疫活性内分泌细胞在草鱼整个肠道中均呈阳性反应;在鲤、青鱼前肠中仅有少量阳性反应;在翘嘴红鲌肠道中未见阳性反应。胃蛋白酶原(Pepsinogen)、凝乳酶(Prochymosin)和神经特异烯醇酶(Neuron specific enolase)3种抗血清在4种鱼的肠道中均未发现阳性反应.本文重点描述草鱼高血糖素免疫活性内分泌细胞在肠道各段的分布,密度及其形态学特征,还就其可能的生理功能与草食性哺乳动物胃肠道中该类内分泌细胞进行比较和讨论。展开更多
通过高效液相色谱法建立不同鲤科鱼HPLC指纹图谱。运用Agilent 1260 DAD-HPLC,Diamousil plus C18-A(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,检测波长230 nm、柱温30℃、进样量20μL、流速0.6 m L/min;...通过高效液相色谱法建立不同鲤科鱼HPLC指纹图谱。运用Agilent 1260 DAD-HPLC,Diamousil plus C18-A(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,检测波长230 nm、柱温30℃、进样量20μL、流速0.6 m L/min;采用指纹图谱相似度评价软件系统(2012版)建立13批鲤科鱼HPLC指纹图谱。试验建立18个共有峰的指纹图谱共有模式;相似度分析表明,除S_(5)、S_(7)、S_(8)这3个产地的相似度在0.850~0.900之间,其余10个产地鲤鱼HPLC指纹图谱的相似度>0.900,表明各产地的化学成分相似度良好。综合试验结果分析,按品质质量将13批鲤科鱼分为3类,第1类为S_(1),S_(8)和S_(9),第2类为S4,S_(1)0,S_(11)和S_(12),第3类为S_(2),S_(3),S_(5),S_(6),S_(7)和S_(13)。指纹图谱建立和化学模式的识别为鱼类品质质量控制提供方法参考。展开更多
Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone fro...Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH GS, rcsGH GS, reGH HP55 and free reGH or uncoated rcsGH were administered to red sea bream. Feeding of reGH GS, reGH HP55 and rcsGH GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.展开更多
文摘应用过氧化物酶抗过氧化物酶(PAP)免疫组织化学技术,用4种哺乳动物中培育出的抗血清对草鱼、青鱼、鲤和翘嘴红鲌4种鲤科鱼的肠内分泌细胞进行免疫、组织化学的鉴别和定位.证实了高血糖素免疫活性内分泌细胞在草鱼整个肠道中均呈阳性反应;在鲤、青鱼前肠中仅有少量阳性反应;在翘嘴红鲌肠道中未见阳性反应。胃蛋白酶原(Pepsinogen)、凝乳酶(Prochymosin)和神经特异烯醇酶(Neuron specific enolase)3种抗血清在4种鱼的肠道中均未发现阳性反应.本文重点描述草鱼高血糖素免疫活性内分泌细胞在肠道各段的分布,密度及其形态学特征,还就其可能的生理功能与草食性哺乳动物胃肠道中该类内分泌细胞进行比较和讨论。
文摘通过高效液相色谱法建立不同鲤科鱼HPLC指纹图谱。运用Agilent 1260 DAD-HPLC,Diamousil plus C18-A(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,检测波长230 nm、柱温30℃、进样量20μL、流速0.6 m L/min;采用指纹图谱相似度评价软件系统(2012版)建立13批鲤科鱼HPLC指纹图谱。试验建立18个共有峰的指纹图谱共有模式;相似度分析表明,除S_(5)、S_(7)、S_(8)这3个产地的相似度在0.850~0.900之间,其余10个产地鲤鱼HPLC指纹图谱的相似度>0.900,表明各产地的化学成分相似度良好。综合试验结果分析,按品质质量将13批鲤科鱼分为3类,第1类为S_(1),S_(8)和S_(9),第2类为S4,S_(1)0,S_(11)和S_(12),第3类为S_(2),S_(3),S_(5),S_(6),S_(7)和S_(13)。指纹图谱建立和化学模式的识别为鱼类品质质量控制提供方法参考。
文摘Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH GS, rcsGH GS, reGH HP55 and free reGH or uncoated rcsGH were administered to red sea bream. Feeding of reGH GS, reGH HP55 and rcsGH GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.
