The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopol...The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopolysaccharides during the degrading process were analyzed by cellulose polyacetate strip electrophoresis and agarose gel electrophoresis.The results showed that the extraction of mucopolysaccharides was increased with the increase of extraction time,alkaline concentration and the addition of cartilage.According to the different electrophoretic migrations,the preparative mucopolysaccharides composed of different polysaccharides with electronegativity can be divided into two parts,the chondroitin sulfate with higher migration and the other with lower migration.The lower part is unstable and easily degraded with alkaline solution.展开更多
Effects of basic fibroblast growth factor (bFGF) and insulin like growth factor II (IGF II) on cartilage cells from proboscis of skate, Raja porasa Günther, were investigated in this study. The cartilage cells we...Effects of basic fibroblast growth factor (bFGF) and insulin like growth factor II (IGF II) on cartilage cells from proboscis of skate, Raja porasa Günther, were investigated in this study. The cartilage cells were cultured in 20% FBS supplemented MEM medium at 24℃. Twelve hours after culture initiation, the cartilage cells were treated with bFGF and IGF II at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF II was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF II together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF II together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7.展开更多
文摘The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopolysaccharides during the degrading process were analyzed by cellulose polyacetate strip electrophoresis and agarose gel electrophoresis.The results showed that the extraction of mucopolysaccharides was increased with the increase of extraction time,alkaline concentration and the addition of cartilage.According to the different electrophoretic migrations,the preparative mucopolysaccharides composed of different polysaccharides with electronegativity can be divided into two parts,the chondroitin sulfate with higher migration and the other with lower migration.The lower part is unstable and easily degraded with alkaline solution.
文摘Effects of basic fibroblast growth factor (bFGF) and insulin like growth factor II (IGF II) on cartilage cells from proboscis of skate, Raja porasa Günther, were investigated in this study. The cartilage cells were cultured in 20% FBS supplemented MEM medium at 24℃. Twelve hours after culture initiation, the cartilage cells were treated with bFGF and IGF II at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF II was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF II together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF II together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7.
