依2450尾短吻舌鳎渔业生物学测定和耳石观测,数理分析得出,耳石轮径与鱼体全长呈线性相关,与纯体重呈显著幂函数关系。运用过程分析法确认年轮形成以年为周期,主要为4～5月。经二次线性拟合年间 Von Bcrtalanffy 生长方程:雌鱼L_t=350.8...依2450尾短吻舌鳎渔业生物学测定和耳石观测,数理分析得出,耳石轮径与鱼体全长呈线性相关,与纯体重呈显著幂函数关系。运用过程分析法确认年轮形成以年为周期,主要为4～5月。经二次线性拟合年间 Von Bcrtalanffy 生长方程:雌鱼L_t=350.8996[1—e^(-O.3965(t+0.7574)], W_t=269.1963[1—e^(-0.392(t+0.7165)]3.1175;雄鱼L_t=314.0091[1—e^(-0.3444(t+1.1236))],W_t=191.6984[1—e^(-0.3263(t+0.9666)]~2.8788胶州湾水域短吻舌鳎年龄构成0～Ⅷ龄,Ⅱ龄鱼占优势(34.06％)。展开更多
This paper presents a new identified species belonging to Lepocreadiidae.The type specimens are deposited in Department of Biology, Nankai University, Ti- anjin. Lepocreadioides cynoglossi sp.nov.(fig.) The measure is...This paper presents a new identified species belonging to Lepocreadiidae.The type specimens are deposited in Department of Biology, Nankai University, Ti- anjin. Lepocreadioides cynoglossi sp.nov.(fig.) The measure is given in millimeters. Qiu Zhaozhi Zhang Runsheng Li Qinkui Body oval, 2.042—2.062 long, 1.455—1.749 in maximum wide. Oral sucker su- bterminal, 0.202—0.212×0.152—0.192. Pharynx 0.081—0.101×0.121—0.152. Esop- hagus short,ceca reaching to near posterior end of the body,Acetabulum 0.283- 0.324×0.222—0.234, situated at somewhat anterior of equater line. Testes sausage like, diagonal, left testis 0.455—0.597×0.111—0.121, right te- stis 0.404—0.485×0.101—0.121. Cirrus pouch saccular, enclosing seminal vesicle, prostatic complex and cirrus. External seminal vesicle preacetabulum, 0.234—0.283 ×0.131—0.142, internal seminal vesicle 0.152—0.192×0.111—0.131. Genital pore on left side of oral sucker. ovary trilobate,0.293—0.303×0.101—0.121,situated at anterior margin of left testis. Seminal receptacle oval 0.313—0.333×0.121—0.142, behind the aceta- bulum. Uterus extending from ovary to metraterm. Vitelline follicles extending from bifurcating level to near posterior end of the body. Eggs 0.038—0.066×0.028— 0.033. This species most closely resembles L.indicus Srivastava, 1941 and L.cynoglossi Fischal et Thomas, 1970, but differs from them in: (1) size of the body, (2) tatio of the suckers, (3) position of the acetabulum, (4) shape and size of the testes, (5) position of the seminal receptacle and uterus. Host: Cynoglossus semilaevis Location: intestine Date: April, 1985 Locality: Bohai Sea, Tianjin, China Type specimen: 1, paratype specimen 8.展开更多
940744 季节对鳎(Sloea solea)摄食习性的影响=Influence of season on the feeding habits ofthe common sole Solea solea[刊,英]/MolinersA,Flos R//Nutr.Abst.Rev.(Series B).—1993,63(5).-2596本研究测定了全年中鳎鱼的饵料组成,
Studies were conducted to determine the cause of the acute mortality of half-smooth tongue sole Cynoglossus semilaevis Günther juveniles in a fish farm in Jimo, Shandong Province, China, in June 2006. Gross signs...Studies were conducted to determine the cause of the acute mortality of half-smooth tongue sole Cynoglossus semilaevis Günther juveniles in a fish farm in Jimo, Shandong Province, China, in June 2006. Gross signs of the diseased tongue sole included several petechiae and ecchymoses on the body and fin necrosis and hemorrhagic lesion at the base of the fin. Bacteria were isolated from kidney, liver and hemorrhagic lesions of the diseased tongue sole. Among 14 strains, SJ060621 was proved to be highly virulent to juvenile tongue sole with LD50 value of 〈1.0×10^5 colony forming units （CFU）mL^-1, while the remaining 13 were avirulent. Among the 16 antibiotics tested, SJ060621 was sensitive to gentamicin and nitrofurantoin. It was identified as Listonella anguillantm with conventional plate and tube tests in combination with API 20E analysis. 16S rRNA gene and partial HSP60 gene sequenceing analysis revealed that the strain was highly homologous with L. anguillarum. Examination of the infected musculature by electron microscopy indicated numerous bacteria and lots of macrophages containing phagocytosed bacteria. Histopathological investigations revealed severe necrotic degenerative changes in the infected organs. Indirect immunofluorescence assay （IFA） was employed to detect the location of occurrence of bacteria, and bacteria were found in aggregations in the inflammatory areas in musculature.展开更多
A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(EST) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequence...A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(EST) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats(SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats(51.4%) are the most abundant class of SSR in the dataset. The dimeric,trimeric,tetrameric and hexameric re-peats are represented in decreasing proportions of 27.2%,16.0%,2.8% and 1.9%,respectively. The frequency of pentameric repeats was observed the least(only 0.7%) . Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely,trimeric repeats are the highest,accounting for 57% of the total microsatellites.展开更多
文摘依2450尾短吻舌鳎渔业生物学测定和耳石观测,数理分析得出,耳石轮径与鱼体全长呈线性相关,与纯体重呈显著幂函数关系。运用过程分析法确认年轮形成以年为周期,主要为4～5月。经二次线性拟合年间 Von Bcrtalanffy 生长方程:雌鱼L_t=350.8996[1—e^(-O.3965(t+0.7574)], W_t=269.1963[1—e^(-0.392(t+0.7165)]3.1175;雄鱼L_t=314.0091[1—e^(-0.3444(t+1.1236))],W_t=191.6984[1—e^(-0.3263(t+0.9666)]~2.8788胶州湾水域短吻舌鳎年龄构成0～Ⅷ龄,Ⅱ龄鱼占优势(34.06％)。
文摘This paper presents a new identified species belonging to Lepocreadiidae.The type specimens are deposited in Department of Biology, Nankai University, Ti- anjin. Lepocreadioides cynoglossi sp.nov.(fig.) The measure is given in millimeters. Qiu Zhaozhi Zhang Runsheng Li Qinkui Body oval, 2.042—2.062 long, 1.455—1.749 in maximum wide. Oral sucker su- bterminal, 0.202—0.212×0.152—0.192. Pharynx 0.081—0.101×0.121—0.152. Esop- hagus short,ceca reaching to near posterior end of the body,Acetabulum 0.283- 0.324×0.222—0.234, situated at somewhat anterior of equater line. Testes sausage like, diagonal, left testis 0.455—0.597×0.111—0.121, right te- stis 0.404—0.485×0.101—0.121. Cirrus pouch saccular, enclosing seminal vesicle, prostatic complex and cirrus. External seminal vesicle preacetabulum, 0.234—0.283 ×0.131—0.142, internal seminal vesicle 0.152—0.192×0.111—0.131. Genital pore on left side of oral sucker. ovary trilobate,0.293—0.303×0.101—0.121,situated at anterior margin of left testis. Seminal receptacle oval 0.313—0.333×0.121—0.142, behind the aceta- bulum. Uterus extending from ovary to metraterm. Vitelline follicles extending from bifurcating level to near posterior end of the body. Eggs 0.038—0.066×0.028— 0.033. This species most closely resembles L.indicus Srivastava, 1941 and L.cynoglossi Fischal et Thomas, 1970, but differs from them in: (1) size of the body, (2) tatio of the suckers, (3) position of the acetabulum, (4) shape and size of the testes, (5) position of the seminal receptacle and uterus. Host: Cynoglossus semilaevis Location: intestine Date: April, 1985 Locality: Bohai Sea, Tianjin, China Type specimen: 1, paratype specimen 8.
文摘940744 季节对鳎(Sloea solea)摄食习性的影响=Influence of season on the feeding habits ofthe common sole Solea solea[刊,英]/MolinersA,Flos R//Nutr.Abst.Rev.(Series B).—1993,63(5).-2596本研究测定了全年中鳎鱼的饵料组成,
基金This study was supported by National High Technology Development Program of China(863,Grant 2006AA100306)the Science Foundation of Shandong Province(032070104).
文摘Studies were conducted to determine the cause of the acute mortality of half-smooth tongue sole Cynoglossus semilaevis Günther juveniles in a fish farm in Jimo, Shandong Province, China, in June 2006. Gross signs of the diseased tongue sole included several petechiae and ecchymoses on the body and fin necrosis and hemorrhagic lesion at the base of the fin. Bacteria were isolated from kidney, liver and hemorrhagic lesions of the diseased tongue sole. Among 14 strains, SJ060621 was proved to be highly virulent to juvenile tongue sole with LD50 value of 〈1.0×10^5 colony forming units （CFU）mL^-1, while the remaining 13 were avirulent. Among the 16 antibiotics tested, SJ060621 was sensitive to gentamicin and nitrofurantoin. It was identified as Listonella anguillantm with conventional plate and tube tests in combination with API 20E analysis. 16S rRNA gene and partial HSP60 gene sequenceing analysis revealed that the strain was highly homologous with L. anguillarum. Examination of the infected musculature by electron microscopy indicated numerous bacteria and lots of macrophages containing phagocytosed bacteria. Histopathological investigations revealed severe necrotic degenerative changes in the infected organs. Indirect immunofluorescence assay （IFA） was employed to detect the location of occurrence of bacteria, and bacteria were found in aggregations in the inflammatory areas in musculature.
基金This work was supported by grants from the Key Laboratory of Marine and Estuarine Fisheries,the Ministry of Agriculture(05-03-01)the Key Laboratory of Aquatic Genetic Resources and Aquaculmral Ecology(AGRA)Certified by the Ministry of Agriculture(KFT2006-5)+1 种基金Shandong Postdoctoral Science Foundation(200601001)China Postdoctoral Science Foundation(20060390999).
文摘A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(EST) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats(SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats(51.4%) are the most abundant class of SSR in the dataset. The dimeric,trimeric,tetrameric and hexameric re-peats are represented in decreasing proportions of 27.2%,16.0%,2.8% and 1.9%,respectively. The frequency of pentameric repeats was observed the least(only 0.7%) . Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely,trimeric repeats are the highest,accounting for 57% of the total microsatellites.
