We investigated the complete mitochondrial genome(mitogenome) of Argyreus hyperbius.The 151 56 bp long genome harbored the gene content(13 protein coding genes,22 tRNA genes,2 rRNA genes and an A+T-rich region) a...We investigated the complete mitochondrial genome(mitogenome) of Argyreus hyperbius.The 151 56 bp long genome harbored the gene content(13 protein coding genes,22 tRNA genes,2 rRNA genes and an A+T-rich region) and the gene arrangement was identical to all known lepidopteran mitogenomes.Mitogenome sequence nucleotide organization and codon usage analyses showed that the genome had a strong A+T bias,accounting for A+T content of 80.8%,with a small negative AT skew(?0.019).Eleven intergenic spacers totaling 96 bp,and 14 overlapping regions totaling 34 bp were scattered throughout the whole genome.As has been observed in other lepidopteran species,12 of the 13 protein-coding genes(PCGs) were initiated by ATN codons,while the COI gene was tentatively designated by the CGA codon.A total of 11 PCGs harbored the complete termination codon TAA,while the COI and COII genes ended at a single T residue.All of the 22 tRNA genes showed typical clover structures except that the tRNASer(AGN) lacks the dihydrouridine(DHU) stem which is replaced by a simple loop.The intergenic spacer sequence between the tRNASer(AGN) and ND1 also contained the ATACTAA motif,which is conserved in all other lepidopterans as well.Additionally,the 349 bp A+T-rich region was not comprised of large tandem repetitive sequences,but harbored a few structures common to other lepidopteran insects,such as the motif ATAGA followed by a 20 bp poly-T stretch,a microsatellite-like(AT)9 element preceded by the ATTTA motif,and a 5 bp poly-A site present immediately upstream of tRNAMet.The mitochondrial genomic sequence features found in this study not only contribute to genetic diversity information of the group,but also are useful in future studies of the endangered nymphalid butterfly in population genetic dynamics,species conservation,phylogeography and evolution.展开更多
The complete mitochondrial genome of Apatura ilia (GenBank accession no. JF437925) was determined as a circular DNA molecule of 15 242 bp, with common genes of 13 putative proteins, 2 rRNAs, and 22 tRNAs and of the ...The complete mitochondrial genome of Apatura ilia (GenBank accession no. JF437925) was determined as a circular DNA molecule of 15 242 bp, with common genes of 13 putative proteins, 2 rRNAs, and 22 tRNAs and of the same gene arrangement as in other sequenced lepidopterans. All protein-coding genes had the typical start codon ATN, except for the COI's using CGA as its start codon as previously demonstrated in other lepidopteran species. The comparison of the nucleotide sequences of the A. ilia mitogenome with ten other Nymphalidae species showed nearly identical gene orientation and arrangement, with only a few alterations in non-coding fragments. The nucleotide composition and codon frequency all fell into the range estimated for the order Lepidoptera. The A. ilia mitochondrial genome had the canonical set of 22 tRNA genes folded in the typical cloverleaf structure, with an unique exception of tRNAs^r (AGN). The mitochondrial genes from A. ilia were overlapped in a total of 33 bp at 9 locations, as well as interleaved with a total of 155 bp intergenic spacers, spread over 12 regions with the size ranging from 1 to 49 bp. Furthermore, the spacer between ND6 and Cyt b harbored a microsatellite-like repeat (TA)23 not found in other completely sequenced nymphalid genomes. The 403 bp AT-rich region harbored two conserved motifs (ATAGA, ATTTA), a 21 bp polyT stretch, a 10 bp poly-A region, along with two microsatellite-like repeats ( (TA)10 and (TA)7), as detected in other nymphalid butterflies.展开更多
The complete mitochondrial genome of the Parathyma sulpitia (Lepidoptera, Nymphalidae, Limenitidinae) was determined. The entire mitochondrial DNA (mtDNA) molecule was 15 268 bp in size. Its gene content and organ...The complete mitochondrial genome of the Parathyma sulpitia (Lepidoptera, Nymphalidae, Limenitidinae) was determined. The entire mitochondrial DNA (mtDNA) molecule was 15 268 bp in size. Its gene content and organization were the same as those of other lepidopteran species, except for the presence of the 121 bp long intergenic spacer between trnSI(AGN)and trnE. The 13 protein-coding genes (PCGs) started with the typical ATN codon, with the exception of the coxl gene that used CGA as its initial codon. In addition, all protein-coding genes terminated at the common stop codon TAA, except the nad4 gene which used a single T as its terminating codon. All 22 tRNA genes possessed the typical clover leaf secondary structure except for trnSI(AGN), which had a simple loop with the absence of the DHU stem. Excluding the A+T-rich region, the mtDNA genome of P. sulpitia harbored 11 intergenic spacers, the longest of which was 121 bp long with the highest A+T content (100%), located between trnSI(AGN) and trnE. As in other lepidopteran species, there was an 18-bp poly-T stretch at the 3'-end of the A+T-rich region, and there were a few short microsatellite-like repeat regions without conspicuous macro-repeats in the A+T-rich region. The phylogenetic analyses of the published complete mt genomes from nine Nymphalidae species were conducted using the concatenated sequences of 13 PCGs with maximum likelihood and Bayesian inference methods. The results indicated that Limenitidinae was a sister to the Heliconiinae among the main Nymphalidae lineages in this study, strongly supporting the results of previous molecular data, while contradicting speculations based on morphological characters.展开更多
Two new species, Gypsochares bicruris sp. nov. and Gypsochares longispinus sp. nov., are described in this paper. The male genital structures of the new species are figured. A checklist of the described species in the...Two new species, Gypsochares bicruris sp. nov. and Gypsochares longispinus sp. nov., are described in this paper. The male genital structures of the new species are figured. A checklist of the described species in the world and a key to the Chinese species are given. The type specimens are deposited in the Department of Biology, Nankai University, Tianjin, China.展开更多
This paper reports seven species of the genus Nymphicula Snellen from China. Nymphicula concaviuscula, sp. nov. is described as new to science. Nymphicula saigusai Yoshiyasu is reported for the first time from China. ...This paper reports seven species of the genus Nymphicula Snellen from China. Nymphicula concaviuscula, sp. nov. is described as new to science. Nymphicula saigusai Yoshiyasu is reported for the first time from China. The genitalia of the new species are illustrated. A key to the Chinese species of Nymphicula is provided.展开更多
Based on 61 specimens reared from leaf-mining larvae on Carmona microphylla in Guangdong, Dialectica geometra (Meyrick, 1916), which represents the genus Dialectica Walsingham newly recorded from China, is described...Based on 61 specimens reared from leaf-mining larvae on Carmona microphylla in Guangdong, Dialectica geometra (Meyrick, 1916), which represents the genus Dialectica Walsingham newly recorded from China, is described and illustrated, and its DNA barcode was cloned and registered. The specimens are deposited in Hunan Agricultural University, Changsha and Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai, China (ZHCIQ).展开更多
The genus Fuscoptilia is reviewed in this paper. Fuscoptilia hoenei Arenberger, a new synonym of Fuscoptilia sinuata (Qin & Zheng), is established. The females of Fuscoptilia sinuata (Qin & Z...The genus Fuscoptilia is reviewed in this paper. Fuscoptilia hoenei Arenberger, a new synonym of Fuscoptilia sinuata (Qin & Zheng), is established. The females of Fuscoptilia sinuata (Qin & Zheng) and Fuscoptilia jarosi Arenberger are described for the first time in science. The adults are photographed, and the male and female genital structures are figured. A key to the described species in the world is given.展开更多
A newly recorded genus Zamarada Moore, 1887 of Geometridae and a newly recorded species Z. excisa Hampson, 1891 from China are reported. Morphological descriptions and illustrations are given.
The last-instar larval external morphologies of Illiberis tenuis (Butler), Chalcosia remota (Walker) and Pryeria sinica Moore of Zygaenidae are described and illustrated. All the specimens are deposited in the Insect ...The last-instar larval external morphologies of Illiberis tenuis (Butler), Chalcosia remota (Walker) and Pryeria sinica Moore of Zygaenidae are described and illustrated. All the specimens are deposited in the Insect Collection of Department of Forest Resources Protection, Kangwon National University, Korea.展开更多
The skipper genus Pelopidas contains 11 species worldwide. 7 species are recorded in China. Pelopidas grisemarginata from Ningqiang County,Shaanxi Province,China is described and illustrated as a new species.
Three new species of the genus Meleonoma are described in this paper: Meleonoma polychaeta sp. nov., Meleonoma foliata sp. nov. and Meleonoma echinata sp. nov. The photographs of the adults and the genital structures ...Three new species of the genus Meleonoma are described in this paper: Meleonoma polychaeta sp. nov., Meleonoma foliata sp. nov. and Meleonoma echinata sp. nov. The photographs of the adults and the genital structures are provided. The type specimens are deposited in the Department of Biology, Nankai University, Tianjin.展开更多
The present paper reports the genus Luquetia Leraut for the first time from China, with Luquetia largimacularis, sp. nov. described as new to science. Another species Luquetia lobella (Denis & Schiffer...The present paper reports the genus Luquetia Leraut for the first time from China, with Luquetia largimacularis, sp. nov. described as new to science. Another species Luquetia lobella (Denis & Schiffermller) is newly recorded from this country. The genital structures are illustrated.展开更多
A new species of the ant genus Epitritus Emery, E. dayui sp. nov., is collected in Xishuangbanna Nature Reserve, Yunnan Province, China. Up to date, 4 species of the genus are known in China: E. hexamerus ...A new species of the ant genus Epitritus Emery, E. dayui sp. nov., is collected in Xishuangbanna Nature Reserve, Yunnan Province, China. Up to date, 4 species of the genus are known in China: E. hexamerus Brown, E. formosus Terayama, Lin et Wu, E. hirashimai Ogata, and E. dayui sp. nov. A key based on worker and female castes is proposed for the 4 known species of Epitritus of East Asia.展开更多
The present paper describes 5 new species of Tribe Grapholitini, viz. Grapholita curviphalla Liu et Yan, sp. nov., G. dactyla Liu et Yan, sp. nov., G. globovalva Liu et Yan, sp. nov., G. globella Liu e...The present paper describes 5 new species of Tribe Grapholitini, viz. Grapholita curviphalla Liu et Yan, sp. nov., G. dactyla Liu et Yan, sp. nov., G. globovalva Liu et Yan, sp. nov., G. globella Liu et Yan, sp. nov. and Cydia curvivalva Liu et Yan, sp. nov.. All the types specimens are deposited in the Institute of Zoology, Academia Sinica.展开更多
The last-stage larval external morphologies of Pleuroptya rulalis (Scopoli), Pleuroptya harutai (Inoue) and Botyodes diniasalis (Walker) of Pyraustinae are described and illustrated. All specimens are deposited ...The last-stage larval external morphologies of Pleuroptya rulalis (Scopoli), Pleuroptya harutai (Inoue) and Botyodes diniasalis (Walker) of Pyraustinae are described and illustrated. All specimens are deposited in the Insect Collection of Department of Forest Resources Protection, Kangwon National University, Korea.展开更多
The last-instar larval external morphologies of Orthaga achatina Butler, Macalla sp. and Locastra muscosalis (Walker) of Epipaschiinae are described and illustrated. All specimens examined are deposited in the Colle...The last-instar larval external morphologies of Orthaga achatina Butler, Macalla sp. and Locastra muscosalis (Walker) of Epipaschiinae are described and illustrated. All specimens examined are deposited in the Collection of College of Life Sciences, China Jiliang University, China. Biological information such as living habit also is included.展开更多
A new genus Unilepidotricha gen. nov. is proposed based on the type species U. gracilicurva sp. nov. collected from Yunnan, China. The photographs of the adult and the genitalia as well as the drawings of the wing ven...A new genus Unilepidotricha gen. nov. is proposed based on the type species U. gracilicurva sp. nov. collected from Yunnan, China. The photographs of the adult and the genitalia as well as the drawings of the wing venation are provided.展开更多
In this paper a new species of Collembola, Tomocerus (Tomocerina) wang-langensis, from Sichuan Province, China is described. It is alike to T.(Tomocerina) calceus Liu, Hou and Li 1999. All the type specimens are depos...In this paper a new species of Collembola, Tomocerus (Tomocerina) wang-langensis, from Sichuan Province, China is described. It is alike to T.(Tomocerina) calceus Liu, Hou and Li 1999. All the type specimens are deposited in the College of Life and Engineering, Southwest University of Science and Technology.展开更多
This paper deals with two species of the genus Antichlidas Meyrick. Antichlidas trigonia sp. nov. is described as new to science. The adult photograph and the genital structures of the new species are provided. A key ...This paper deals with two species of the genus Antichlidas Meyrick. Antichlidas trigonia sp. nov. is described as new to science. The adult photograph and the genital structures of the new species are provided. A key to the described species is given.展开更多
基金supported by the Provincial Key Project of the Natural Science Foundation from Anhui Province,China (KJ2010A142)the Chinese Academy of Sciences(KZCX22YW2JC104)the CAS/SAFEA International Partnership Program for Creative Research Teams,and the State Key Laboratory of Palaeobiology and Stratigraphy,Nanjing Institute of Geology and Palaeontology,Chinese Academy of Sciences(104143)~~
文摘We investigated the complete mitochondrial genome(mitogenome) of Argyreus hyperbius.The 151 56 bp long genome harbored the gene content(13 protein coding genes,22 tRNA genes,2 rRNA genes and an A+T-rich region) and the gene arrangement was identical to all known lepidopteran mitogenomes.Mitogenome sequence nucleotide organization and codon usage analyses showed that the genome had a strong A+T bias,accounting for A+T content of 80.8%,with a small negative AT skew(?0.019).Eleven intergenic spacers totaling 96 bp,and 14 overlapping regions totaling 34 bp were scattered throughout the whole genome.As has been observed in other lepidopteran species,12 of the 13 protein-coding genes(PCGs) were initiated by ATN codons,while the COI gene was tentatively designated by the CGA codon.A total of 11 PCGs harbored the complete termination codon TAA,while the COI and COII genes ended at a single T residue.All of the 22 tRNA genes showed typical clover structures except that the tRNASer(AGN) lacks the dihydrouridine(DHU) stem which is replaced by a simple loop.The intergenic spacer sequence between the tRNASer(AGN) and ND1 also contained the ATACTAA motif,which is conserved in all other lepidopterans as well.Additionally,the 349 bp A+T-rich region was not comprised of large tandem repetitive sequences,but harbored a few structures common to other lepidopteran insects,such as the motif ATAGA followed by a 20 bp poly-T stretch,a microsatellite-like(AT)9 element preceded by the ATTTA motif,and a 5 bp poly-A site present immediately upstream of tRNAMet.The mitochondrial genomic sequence features found in this study not only contribute to genetic diversity information of the group,but also are useful in future studies of the endangered nymphalid butterfly in population genetic dynamics,species conservation,phylogeography and evolution.
基金supported by grants from the National Science Foundation of China (41172004,40871034)partially by the Provincial Key Projects of Natural Science Foundation,Colleges of Anhui Province (KJ2010A142)
文摘The complete mitochondrial genome of Apatura ilia (GenBank accession no. JF437925) was determined as a circular DNA molecule of 15 242 bp, with common genes of 13 putative proteins, 2 rRNAs, and 22 tRNAs and of the same gene arrangement as in other sequenced lepidopterans. All protein-coding genes had the typical start codon ATN, except for the COI's using CGA as its start codon as previously demonstrated in other lepidopteran species. The comparison of the nucleotide sequences of the A. ilia mitogenome with ten other Nymphalidae species showed nearly identical gene orientation and arrangement, with only a few alterations in non-coding fragments. The nucleotide composition and codon frequency all fell into the range estimated for the order Lepidoptera. The A. ilia mitochondrial genome had the canonical set of 22 tRNA genes folded in the typical cloverleaf structure, with an unique exception of tRNAs^r (AGN). The mitochondrial genes from A. ilia were overlapped in a total of 33 bp at 9 locations, as well as interleaved with a total of 155 bp intergenic spacers, spread over 12 regions with the size ranging from 1 to 49 bp. Furthermore, the spacer between ND6 and Cyt b harbored a microsatellite-like repeat (TA)23 not found in other completely sequenced nymphalid genomes. The 403 bp AT-rich region harbored two conserved motifs (ATAGA, ATTTA), a 21 bp polyT stretch, a 10 bp poly-A region, along with two microsatellite-like repeats ( (TA)10 and (TA)7), as detected in other nymphalid butterflies.
基金supported by the National Natural Science Foundation of China (41172004)the CAS/SAFEA International Partnership Program for Creative Research Teams,Chinese Academy of Sciences (KZCX22YW2JC104)+1 种基金the Provincial Key Project of the Natural Science Foundation from the Anhui Province, China (KJ2010A142)the Open Funds from the State Key Laboratory of Palaeobiology and Stratigraphy,Nanjing Institute of Geology and Palaeontology,Chinese Academy of Sciences
文摘The complete mitochondrial genome of the Parathyma sulpitia (Lepidoptera, Nymphalidae, Limenitidinae) was determined. The entire mitochondrial DNA (mtDNA) molecule was 15 268 bp in size. Its gene content and organization were the same as those of other lepidopteran species, except for the presence of the 121 bp long intergenic spacer between trnSI(AGN)and trnE. The 13 protein-coding genes (PCGs) started with the typical ATN codon, with the exception of the coxl gene that used CGA as its initial codon. In addition, all protein-coding genes terminated at the common stop codon TAA, except the nad4 gene which used a single T as its terminating codon. All 22 tRNA genes possessed the typical clover leaf secondary structure except for trnSI(AGN), which had a simple loop with the absence of the DHU stem. Excluding the A+T-rich region, the mtDNA genome of P. sulpitia harbored 11 intergenic spacers, the longest of which was 121 bp long with the highest A+T content (100%), located between trnSI(AGN) and trnE. As in other lepidopteran species, there was an 18-bp poly-T stretch at the 3'-end of the A+T-rich region, and there were a few short microsatellite-like repeat regions without conspicuous macro-repeats in the A+T-rich region. The phylogenetic analyses of the published complete mt genomes from nine Nymphalidae species were conducted using the concatenated sequences of 13 PCGs with maximum likelihood and Bayesian inference methods. The results indicated that Limenitidinae was a sister to the Heliconiinae among the main Nymphalidae lineages in this study, strongly supporting the results of previous molecular data, while contradicting speculations based on morphological characters.
文摘Two new species, Gypsochares bicruris sp. nov. and Gypsochares longispinus sp. nov., are described in this paper. The male genital structures of the new species are figured. A checklist of the described species in the world and a key to the Chinese species are given. The type specimens are deposited in the Department of Biology, Nankai University, Tianjin, China.
基金The project was supported by Natural Science Foundation of Tianjin ( 0 2 361 40 1 1 )
文摘This paper reports seven species of the genus Nymphicula Snellen from China. Nymphicula concaviuscula, sp. nov. is described as new to science. Nymphicula saigusai Yoshiyasu is reported for the first time from China. The genitalia of the new species are illustrated. A key to the Chinese species of Nymphicula is provided.
基金supported by General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China (2009IK277)
文摘Based on 61 specimens reared from leaf-mining larvae on Carmona microphylla in Guangdong, Dialectica geometra (Meyrick, 1916), which represents the genus Dialectica Walsingham newly recorded from China, is described and illustrated, and its DNA barcode was cloned and registered. The specimens are deposited in Hunan Agricultural University, Changsha and Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai, China (ZHCIQ).
基金pported by the National Natural Science Foundation of China(30270181)
文摘The genus Fuscoptilia is reviewed in this paper. Fuscoptilia hoenei Arenberger, a new synonym of Fuscoptilia sinuata (Qin & Zheng), is established. The females of Fuscoptilia sinuata (Qin & Zheng) and Fuscoptilia jarosi Arenberger are described for the first time in science. The adults are photographed, and the male and female genital structures are figured. A key to the described species in the world is given.
基金supported by the National Science Foundation of China (31172127,NSFC-J0930004)a grant from the Key Laboratory of the Zoological Systematics and Evolution of the Chinese Academy of Sciences (O529YX5105)
文摘A newly recorded genus Zamarada Moore, 1887 of Geometridae and a newly recorded species Z. excisa Hampson, 1891 from China are reported. Morphological descriptions and illustrations are given.
文摘The last-instar larval external morphologies of Illiberis tenuis (Butler), Chalcosia remota (Walker) and Pryeria sinica Moore of Zygaenidae are described and illustrated. All the specimens are deposited in the Insect Collection of Department of Forest Resources Protection, Kangwon National University, Korea.
基金supported by the National Natural Science Foundation of China (30499341)
文摘The skipper genus Pelopidas contains 11 species worldwide. 7 species are recorded in China. Pelopidas grisemarginata from Ningqiang County,Shaanxi Province,China is described and illustrated as a new species.
文摘Three new species of the genus Meleonoma are described in this paper: Meleonoma polychaeta sp. nov., Meleonoma foliata sp. nov. and Meleonoma echinata sp. nov. The photographs of the adults and the genital structures are provided. The type specimens are deposited in the Department of Biology, Nankai University, Tianjin.
文摘The present paper reports the genus Luquetia Leraut for the first time from China, with Luquetia largimacularis, sp. nov. described as new to science. Another species Luquetia lobella (Denis & Schiffermller) is newly recorded from this country. The genital structures are illustrated.
文摘A new species of the ant genus Epitritus Emery, E. dayui sp. nov., is collected in Xishuangbanna Nature Reserve, Yunnan Province, China. Up to date, 4 species of the genus are known in China: E. hexamerus Brown, E. formosus Terayama, Lin et Wu, E. hirashimai Ogata, and E. dayui sp. nov. A key based on worker and female castes is proposed for the 4 known species of Epitritus of East Asia.
文摘The present paper describes 5 new species of Tribe Grapholitini, viz. Grapholita curviphalla Liu et Yan, sp. nov., G. dactyla Liu et Yan, sp. nov., G. globovalva Liu et Yan, sp. nov., G. globella Liu et Yan, sp. nov. and Cydia curvivalva Liu et Yan, sp. nov.. All the types specimens are deposited in the Institute of Zoology, Academia Sinica.
文摘The last-stage larval external morphologies of Pleuroptya rulalis (Scopoli), Pleuroptya harutai (Inoue) and Botyodes diniasalis (Walker) of Pyraustinae are described and illustrated. All specimens are deposited in the Insect Collection of Department of Forest Resources Protection, Kangwon National University, Korea.
基金The project was supported by the Zhejiang Natural Science Foundation(Y304454)
文摘The last-instar larval external morphologies of Orthaga achatina Butler, Macalla sp. and Locastra muscosalis (Walker) of Epipaschiinae are described and illustrated. All specimens examined are deposited in the Collection of College of Life Sciences, China Jiliang University, China. Biological information such as living habit also is included.
基金The research was supported by the National Natural Science Foundation of China (30670251)
文摘A new genus Unilepidotricha gen. nov. is proposed based on the type species U. gracilicurva sp. nov. collected from Yunnan, China. The photographs of the adult and the genitalia as well as the drawings of the wing venation are provided.
文摘In this paper a new species of Collembola, Tomocerus (Tomocerina) wang-langensis, from Sichuan Province, China is described. It is alike to T.(Tomocerina) calceus Liu, Hou and Li 1999. All the type specimens are deposited in the College of Life and Engineering, Southwest University of Science and Technology.
基金The research was supported by the National Natural Science Foundation of China for the Special Program
文摘This paper deals with two species of the genus Antichlidas Meyrick. Antichlidas trigonia sp. nov. is described as new to science. The adult photograph and the genital structures of the new species are provided. A key to the described species is given.