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不同细胞密度和脉冲电压对鸡红血细胞融合率的影响 被引量:2
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作者 张翠 冯磊 +1 位作者 李秀兰 徐承水 《曲阜师范大学学报(自然科学版)》 CAS 2005年第2期107-109,共3页
以公鸡红血细胞为材料,研究了不同的细胞密度、成串脉冲电压强度、频率和时间及融合脉冲电压强度、宽度和次数等因素对细胞电融合率的影响,结果显示:成串脉冲电压频率在1.0MHZ ,融合脉冲电压场强为130 0V/cm时,细胞密度为1.6×10 5... 以公鸡红血细胞为材料,研究了不同的细胞密度、成串脉冲电压强度、频率和时间及融合脉冲电压强度、宽度和次数等因素对细胞电融合率的影响,结果显示:成串脉冲电压频率在1.0MHZ ,融合脉冲电压场强为130 0V/cm时,细胞密度为1.6×10 5/mL融合效果最好,融合率可达75 .4 2 % .固定融合脉冲电压强度在12 0 0V/cm ,成串脉冲电压在110~12 0V/cm时,融合率在80 %以上。 展开更多
关键词 鸡红血细胞 细胞密度 成串脉冲电压 融合脉冲电压 融合率
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聚乙二醇诱导细胞融合影响因素探究 被引量:14
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作者 李秀兰 姜曰水 《菏泽学院学报》 2007年第5期79-82,共4页
以鸡红血细胞为材料,聚乙二醇(Mr=4000)为诱导剂,研究不同的细胞密度、聚乙二醇体积分数、Ca^2+浓度等因素对细胞融合率的影响,结果显示:细胞密度为(3.0~4.0)×10^4/mL,聚乙二醇的体积分数为50%,Ca^2+浓度为50mmol... 以鸡红血细胞为材料,聚乙二醇(Mr=4000)为诱导剂,研究不同的细胞密度、聚乙二醇体积分数、Ca^2+浓度等因素对细胞融合率的影响,结果显示:细胞密度为(3.0~4.0)×10^4/mL,聚乙二醇的体积分数为50%,Ca^2+浓度为50mmol/mL时融合效果最好,融合率高达29.76%. 展开更多
关键词 鸡红血细胞 细胞密度 聚乙二醇 融合率
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Effects of Imidapril on Venous Blood Gas Values in Broiler Chickens Exposed to Low Ambient Temperature
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作者 Xueqin HAO Meng LI +2 位作者 Shouyan ZHANG Yongshu WANG Tongwen SUN 《Agricultural Science & Technology》 CAS 2013年第7期1002-1004,共3页
ObjectiveThis study was designed to evaluate the effects of imidapril on blood gas parameters in broiler chickens. MethodTwenty-four chickens were randomly divided into three groups (n=8), control group, low temperatu... ObjectiveThis study was designed to evaluate the effects of imidapril on blood gas parameters in broiler chickens. MethodTwenty-four chickens were randomly divided into three groups (n=8), control group, low temperature group and imidapril group. Chickens in low temperature group and imidapril group were exposed to low ambient temperature (12-18 ℃) from age at 14 d to 45 d, whereas the control group was exposed to 24-30 ℃; chickens in imidapril group were gavaged with imidapril (3 mg/kg) once daily for 30 d. At age of 45 d, blood was taken from wing vein and blood gas parameters were evaluated by blood gas analyzer in Luoyang Central Hospital Affiliated to Zhengzhou University. ResultImidapril significantly increased hematocrit (HCT) and total hemoglobin content (T HBC ) and blood Na concentration in broiler chickens exposed to low ambient temperature. No significant differences were observed in pH, P CO 2 , P O 2 , K + , Ca 2+ , HCO 3-, HCO 3std , T CO 2 , BE and SO 2c . ConclusionImidapril increases hematocrit, total hemoglobin content and blood Na + concentration in chickens exposed to low ambient temperature. 展开更多
关键词 IMIDAPRIL Blood gas value Broiler chicken Low ambient temperature
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