胃癌细胞鸡胚移植模型中血清基质金属蛋白酶9检测意义

目的 :测定胃癌细胞接种至鸡胚绒毛尿囊膜后的成瘤过程中鸡胚血清基质金属蛋白酶 9( MMP- 9)含量变化。方法 :利用形态学方法观察胃癌细胞接种至鸡胚绒毛尿囊膜后的成瘤过程 ,并通过酶联免疫吸附测定 ( ELISA)法检测不同时间点鸡胚模型血清中 MMP- 9含量的变化 ,并结合胃癌生物学行为进行分析。结果 :1× 1 0 6·ml-1 胃癌细胞组于接种 72 h后 ,出现肉眼可见独立结节 ,随时间推移 ,结节体积增大 ,且鸡胚绒毛尿囊膜上较大血管向结节处集中 ;1× 1 0 6·ml-1胃癌细胞组 ,72 h后血清 MMP- 9含量明显增高 ,至 7d时达到最高值。结论 :胃癌细胞的成瘤过程中 MMP- 9水解酶含量明显增加 ,此模型用于胃癌特性的研究是可行的。

三氧化二砷对鸡胚移植胆管癌生长的抑制作用

目的:探讨三氧化二砷(As_2O_3)对鸡胚移植胆管癌生长的抑制作用及其机制。方法:建立胆管癌QBC_(939)细胞株鸡胚移植模型,接种癌细胞后9d切取标本,比较As_2O_3作用组和对照组肿瘤生长、血管生成情况,原位末端标记法检测肿瘤组织细胞凋亡,免疫组化检测核增生抗原(PCNA)与血管内皮生长因子(VEGF)的表达。结果:As_2O_3作用组肿瘤生长和血管生成受到抑制,细胞凋亡增多、PCNA和VEGF表达减弱。结论:As_2O_3有抗胆管癌的作用,其机制与诱导细胞凋亡,抑制癌细胞增生、癌组织VEGF表达以及肿瘤血管生成有关。

1缺氧诱导因子-1α反义寡核苷酸对新生血管形成的抑制作用

目的:研究缺氧诱导因子-1α反义寡核苷酸对肿瘤血管形成的影响.方法:通过将腺病毒包装的反义HIF-α(Ad-antiHIF-1α)和腺病毒包装的lacZ标志基因(Ad-lacZ)注射入人大肠癌鸡胚移植模型的鸡胚绒毛尿囊膜(CAM)中,观察他们对鸡胚移植瘤新生血管形成的影响.结果:注射Ad-antiHIF-1α组血管稀疏、纤细,一级血管数(16.8±1.6vs45.2±2.8,t=2.42,P=0.02<0.05)及二级血管数(34.8±3.6vs58.4±5.3,t=2.97,P=0.005<0.01)均明显少于注射Ad-lacZ组.

Establishment of a transplantation tumor model of human osteosarcoma in chick embryo

Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods: Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane (CAM) of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results: The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion: It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.