自从英国剑桥大学 Rose 等确定人体8种必需氨基酸以后,联合国粮农组织(FAO)和世界卫生组织(WHO)提出8种必需氨基酸模式标准,反映世界蛋白质工业水平,成为当今世界上两个权威性的“理想氨基酸模式”。这个模式是以各种氨基酸中含量最少...自从英国剑桥大学 Rose 等确定人体8种必需氨基酸以后,联合国粮农组织(FAO)和世界卫生组织(WHO)提出8种必需氨基酸模式标准,反映世界蛋白质工业水平,成为当今世界上两个权威性的“理想氨基酸模式”。这个模式是以各种氨基酸中含量最少的色氨酸为基数来确定其余7种氨基酸的比例。现在已有更多的发现是,色氨酸的功能是确立氨基酸的一级结构。展开更多
The present research was conducted to extract keratin protein from chicken feathers. Protein is an important nutrient needed by our body to maintain body structures and is an important ingredient for cosmetic products...The present research was conducted to extract keratin protein from chicken feathers. Protein is an important nutrient needed by our body to maintain body structures and is an important ingredient for cosmetic products. Chicken feathers have high level of keratin protein content and can become a suitable protein source. The main processes involved are first dissolving chicken feathers using different reducing agents and later on separating the protein from chemicals. Reducing agents used are potassium cyanide, thioglycolic acid and sodium sulphide. Once the feathers are dissolved using reducing agents, ammonium sulfate solution is added to the solution for the precipitation of protein. The precipitated protein is washed with water several times and sodium hydroxide solution is used to obtain protein back in the solution form. Out of three different reducing agents used, sodium sulfide gives the highest efficiency in dissolving chicken feathers since the feathers are dissolved in a very short period of time. The percentage of keratin protein is evaluated by means of biuret test and FTIR analysis. The analysis by FTIR confirmed the presence of carboxyl acid and amino groups in the protein solution. The biuret test helps in determining the concentration of protein obtained from different methods. Thus these two tests confirm the presence of protein in the solution. From this research, it can be concluded that protein can be extracted from chicken feathers. The keratin protein solution can be used for several purposes such as anti-aging cream, shampoo, and conditioner and for medical purposes such as bone replacement and bone graft.展开更多
文摘自从英国剑桥大学 Rose 等确定人体8种必需氨基酸以后,联合国粮农组织(FAO)和世界卫生组织(WHO)提出8种必需氨基酸模式标准,反映世界蛋白质工业水平,成为当今世界上两个权威性的“理想氨基酸模式”。这个模式是以各种氨基酸中含量最少的色氨酸为基数来确定其余7种氨基酸的比例。现在已有更多的发现是,色氨酸的功能是确立氨基酸的一级结构。
文摘The present research was conducted to extract keratin protein from chicken feathers. Protein is an important nutrient needed by our body to maintain body structures and is an important ingredient for cosmetic products. Chicken feathers have high level of keratin protein content and can become a suitable protein source. The main processes involved are first dissolving chicken feathers using different reducing agents and later on separating the protein from chemicals. Reducing agents used are potassium cyanide, thioglycolic acid and sodium sulphide. Once the feathers are dissolved using reducing agents, ammonium sulfate solution is added to the solution for the precipitation of protein. The precipitated protein is washed with water several times and sodium hydroxide solution is used to obtain protein back in the solution form. Out of three different reducing agents used, sodium sulfide gives the highest efficiency in dissolving chicken feathers since the feathers are dissolved in a very short period of time. The percentage of keratin protein is evaluated by means of biuret test and FTIR analysis. The analysis by FTIR confirmed the presence of carboxyl acid and amino groups in the protein solution. The biuret test helps in determining the concentration of protein obtained from different methods. Thus these two tests confirm the presence of protein in the solution. From this research, it can be concluded that protein can be extracted from chicken feathers. The keratin protein solution can be used for several purposes such as anti-aging cream, shampoo, and conditioner and for medical purposes such as bone replacement and bone graft.