[Objective] This study aimed to investigate the expression of IGF-I mRNA in breast and leg muscles of goose at the embryonic period and early growth stage, as well as the correlations between IGF-I mRNA expression and...[Objective] This study aimed to investigate the expression of IGF-I mRNA in breast and leg muscles of goose at the embryonic period and early growth stage, as well as the correlations between IGF-I mRNA expression and goose body weight. [Method] The expression of IGF-I mRNA in breast and leg muscles of 23, 25, 27, 29-embryo days and 0, 2, 4, 6, 8, 10, 12 week old geese of Taihu and Wanxi breeds was analyzed with multiplex competitive fluorescent-PCR method. [Result] Goose IGF-I mRNA was detected in breast and leg muscles of 23-embryo days of the both goose breeds. There were some differences in the IGF-I mRNA expression profiles between the two goose breeds during development stage. The body weight of Taihu goose was positively related with the IGF-I mRNA expression in leg muscle, and the body weight of Wanxi goose was positively related with IGF-I mRNA level in breast muscle. [Conclusion] IGF-I mRNA in the muscle of goose might play an important role in early development stage.展开更多
[Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple s...[Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple species were aligned in GenBank, and a phylogenetic tree was constructed for homology analysis. [Result] C4B gene in Siji goose shared relatively high homology with chicken and quail; Siji goose C4B gene was expressed highly in liver and lung of adult geese and expressed lowly in epididymis, seminiferous duct, brain, kidney, testis, heart, oviduct and smal intestine. [Conclusion] In the present study, mRNA expression lev-el of C4B gene in different tissues and organs of Siji goose was determined by flu-orescence quantitative PCR, which provided basis for rapid diagnosis of specific an-imal diseases.展开更多
[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny a...[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny and evolution. [Method] Ten geese were selected randomly from the core populations of grey-, mosaic- and white-plumaged Xinjiang Goose respectively with a total number of thirty as experi- mental materials, of which the blood samples were collected from the largest vein under the wing （brachial vein） for DNA extraction. Sequences of mitochondrial DNA D-loop regions were determined using DNA sequencing technology to analyze the polymorphism. In addition, the genetic distances among different populations were estimated through the comparison with the reference sequences. [Resull] The con- tents of A, G, C and T nucleotides in the D-loop region of Xinjiang Goose were 28.85%, 17.05%, 25.38% and 28.72%, respectively. The average haplotype diversity and nucleotide diversity of Xinjiang Goose were 0.583 and 0.056. Xinjiang Goose and Greylag Goose were clustered into the same group. [Conclusion] The results showed that Xinjiang Geese with three different colors of plumage all descend from Greylag Goose （Anser anser）.展开更多
As a central issue in stylistics, foregrounding, which can be achieved by deviation and overregularity at every linguistic level, can give readers more innovative and ingenious perspective on appreciation of text. W. ...As a central issue in stylistics, foregrounding, which can be achieved by deviation and overregularity at every linguistic level, can give readers more innovative and ingenious perspective on appreciation of text. W. B. Yeats＇ Leda and the Swan is a poem deserving close reading and attention. The analysis from sound patterning, meter and rhythm, verbal repetition endows the poem with some novel and new aesthetic significance展开更多
The paper discusses the experimental results pointing to the efficient channel of the CO vibrational to the C2 electronic energy-transfer. The radiation spectra of the d3Hg electronic state of C2 molecule are investig...The paper discusses the experimental results pointing to the efficient channel of the CO vibrational to the C2 electronic energy-transfer. The radiation spectra of the d3Hg electronic state of C2 molecule are investigated and the relation of their kinetics to a vibrational excitation of CO molecules in the He-CO-O2 plasma is discussed. The changes of CO VED （vibrational energy distribution） were imposed by an application of a laser resonator to the discharge tube under investigation. Strong correlation has been found in experiments between concentrations of vibrationally excited CO molecules and the electronically excited state d31qg of C2 molecule. Modulation of the intensity of lasing oscillation cased marked modulation of the intensity of the emission from d3Hg （Swan bands）. It was found that the vibrational levels 12 〈 w 〈 20 of the CO molecules most effect on the concentration of the C2 （d3flg）. The facts discussed above most probably point to the existence of efficient channel for the VE energy transfer： CO （w） ＋ C2 →CO ＋ C2 （d3II）. The efficiency of this channel increases with the growth of populations of high vibrational levels of CO （X1∑, w）.展开更多
基金Supported by the Key Science and Technology Research and Development Program of Jiangsu Province(BE2010370)the Natural Science Foundation of Jiangsu Province (BK2001432)Earmarked Fund for China Agriculture Research System(nycytx-42-G1)~~
文摘[Objective] This study aimed to investigate the expression of IGF-I mRNA in breast and leg muscles of goose at the embryonic period and early growth stage, as well as the correlations between IGF-I mRNA expression and goose body weight. [Method] The expression of IGF-I mRNA in breast and leg muscles of 23, 25, 27, 29-embryo days and 0, 2, 4, 6, 8, 10, 12 week old geese of Taihu and Wanxi breeds was analyzed with multiplex competitive fluorescent-PCR method. [Result] Goose IGF-I mRNA was detected in breast and leg muscles of 23-embryo days of the both goose breeds. There were some differences in the IGF-I mRNA expression profiles between the two goose breeds during development stage. The body weight of Taihu goose was positively related with the IGF-I mRNA expression in leg muscle, and the body weight of Wanxi goose was positively related with IGF-I mRNA level in breast muscle. [Conclusion] IGF-I mRNA in the muscle of goose might play an important role in early development stage.
基金Supported by Agricultural Independent Innovation Fund of Jiangsu Province[CX(09)638]~~
文摘[Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple species were aligned in GenBank, and a phylogenetic tree was constructed for homology analysis. [Result] C4B gene in Siji goose shared relatively high homology with chicken and quail; Siji goose C4B gene was expressed highly in liver and lung of adult geese and expressed lowly in epididymis, seminiferous duct, brain, kidney, testis, heart, oviduct and smal intestine. [Conclusion] In the present study, mRNA expression lev-el of C4B gene in different tissues and organs of Siji goose was determined by flu-orescence quantitative PCR, which provided basis for rapid diagnosis of specific an-imal diseases.
基金Supported by the Fond for Open Projects of Xinjiang Key Laboratory of Herbivore Nutrition for Meat&Milk Production~~
文摘[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny and evolution. [Method] Ten geese were selected randomly from the core populations of grey-, mosaic- and white-plumaged Xinjiang Goose respectively with a total number of thirty as experi- mental materials, of which the blood samples were collected from the largest vein under the wing （brachial vein） for DNA extraction. Sequences of mitochondrial DNA D-loop regions were determined using DNA sequencing technology to analyze the polymorphism. In addition, the genetic distances among different populations were estimated through the comparison with the reference sequences. [Resull] The con- tents of A, G, C and T nucleotides in the D-loop region of Xinjiang Goose were 28.85%, 17.05%, 25.38% and 28.72%, respectively. The average haplotype diversity and nucleotide diversity of Xinjiang Goose were 0.583 and 0.056. Xinjiang Goose and Greylag Goose were clustered into the same group. [Conclusion] The results showed that Xinjiang Geese with three different colors of plumage all descend from Greylag Goose （Anser anser）.
文摘As a central issue in stylistics, foregrounding, which can be achieved by deviation and overregularity at every linguistic level, can give readers more innovative and ingenious perspective on appreciation of text. W. B. Yeats＇ Leda and the Swan is a poem deserving close reading and attention. The analysis from sound patterning, meter and rhythm, verbal repetition endows the poem with some novel and new aesthetic significance
文摘The paper discusses the experimental results pointing to the efficient channel of the CO vibrational to the C2 electronic energy-transfer. The radiation spectra of the d3Hg electronic state of C2 molecule are investigated and the relation of their kinetics to a vibrational excitation of CO molecules in the He-CO-O2 plasma is discussed. The changes of CO VED （vibrational energy distribution） were imposed by an application of a laser resonator to the discharge tube under investigation. Strong correlation has been found in experiments between concentrations of vibrationally excited CO molecules and the electronically excited state d31qg of C2 molecule. Modulation of the intensity of lasing oscillation cased marked modulation of the intensity of the emission from d3Hg （Swan bands）. It was found that the vibrational levels 12 〈 w 〈 20 of the CO molecules most effect on the concentration of the C2 （d3flg）. The facts discussed above most probably point to the existence of efficient channel for the VE energy transfer： CO （w） ＋ C2 →CO ＋ C2 （d3II）. The efficiency of this channel increases with the growth of populations of high vibrational levels of CO （X1∑, w）.
