Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine ...Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine to cervids, a s well as a few loci derived directly from deer microsatellite library. These lo ci have been used in parentage determination, genetic diversity and population s tructure, population introgression, as genetic marker gestation length and winte ring survival et al. However, microsatellite loci presently found are untouchabl e to the demand of application. Future work should include: 1) isolating a large number of cervine microsatellite loci, 2) constructing genetic and physical map s of microsatellite loci. So that microsatelites have a strong base for advanced applications in deer.展开更多
A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes s...A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.展开更多
文摘Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine to cervids, a s well as a few loci derived directly from deer microsatellite library. These lo ci have been used in parentage determination, genetic diversity and population s tructure, population introgression, as genetic marker gestation length and winte ring survival et al. However, microsatellite loci presently found are untouchabl e to the demand of application. Future work should include: 1) isolating a large number of cervine microsatellite loci, 2) constructing genetic and physical map s of microsatellite loci. So that microsatelites have a strong base for advanced applications in deer.
文摘A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.
