Nitrogen is one of the most needed elements by coffee plants. Utilization of biological nitrogen fixation by non symbiotic bacteria offers alternative to reduce the N fertilizer usage. This study was focused to obtain...Nitrogen is one of the most needed elements by coffee plants. Utilization of biological nitrogen fixation by non symbiotic bacteria offers alternative to reduce the N fertilizer usage. This study was focused to obtain aerobic non symbiotic nitrogen-fixing bacteria from coffee rhizosphere. The application of those bacteria was expected to enhance coffee seedling growth. Sixty four aerobic nitrogen-fixing bacterial isolates were isolated from coffee plants rhizosphere from Jember, East Java using several nitrogen free medium, such as Ashby, malate acid, and fahreus agar. The nitrogen fixation ability of the isolates was determined by measuring their ability in pellicle formation on semi solid medium and ammonium excretion on growth medium. Ab Kws.l, Asm E6s.3.a, Asm Bsl.1, and Asm E6s were the isolates which showed the best performance on nitrogen fixation with excreted ammonium concentration ranged from 129.6 up to 239.8 pM/mg dry weight cell. Acetylene reduction assay was used to detect nitrogenase activity. Ab Kws.1 was the isolate which had the highest nitrogenase activity （7.4 mmol N2 fixed/gram dry weight cell/hour）. Inoculation of the four best isolates onto Robusta coffee seedling positively enhanced the seedling growth in this green house experiment. Based on the results of Becton Dickinson＇s （BD） PhoenixTM Automated Microbiology System biochemical tests, Asm Bls.l isolates has similarities with Achromobacter sp., Asm E6s.l and Asm E6s.3.a had similarities with Stenotrophomonas maltophilia, while Ab Kws. 1 had similarities with Leifsonia aquatica.展开更多
Objective： To study the effect of puncturing points Taiyang （Ex-HN 5）, Jiaosun （TE 20）, Qiuxu （GB 40） and Shenmai （BL 62） on migraine. Methods： The 120 cases in the treatment group were treated with routine ...Objective： To study the effect of puncturing points Taiyang （Ex-HN 5）, Jiaosun （TE 20）, Qiuxu （GB 40） and Shenmai （BL 62） on migraine. Methods： The 120 cases in the treatment group were treated with routine acupuncture therapy, while the 40 cases in the control group were treated with Ergotamine and Caffeine Tablet, and then the therapeutic effect and recovery time of the two groups were compared. Results： The recovery rate of the treatment group was 90.0%, which is higher than 70.0% of the control group （P〈 0.01）. The total effective rate of the treatment group was 100%, which is better than 92.5% of the control group （P 〈 0.05）, The therapeutic effect of the treatment group was significantly better than the control group and the treatment group needed shorter treatment period （P 〈 0.05）. Conclusions： Acupuncture has higher recovery rate and total effective rate as well as shorter treatment period than oral ergotamine and caffeine tablets, which indicated that acupuncture is superior to the routinely-administered oral western tablets in treating migraine.展开更多
文摘Nitrogen is one of the most needed elements by coffee plants. Utilization of biological nitrogen fixation by non symbiotic bacteria offers alternative to reduce the N fertilizer usage. This study was focused to obtain aerobic non symbiotic nitrogen-fixing bacteria from coffee rhizosphere. The application of those bacteria was expected to enhance coffee seedling growth. Sixty four aerobic nitrogen-fixing bacterial isolates were isolated from coffee plants rhizosphere from Jember, East Java using several nitrogen free medium, such as Ashby, malate acid, and fahreus agar. The nitrogen fixation ability of the isolates was determined by measuring their ability in pellicle formation on semi solid medium and ammonium excretion on growth medium. Ab Kws.l, Asm E6s.3.a, Asm Bsl.1, and Asm E6s were the isolates which showed the best performance on nitrogen fixation with excreted ammonium concentration ranged from 129.6 up to 239.8 pM/mg dry weight cell. Acetylene reduction assay was used to detect nitrogenase activity. Ab Kws.1 was the isolate which had the highest nitrogenase activity （7.4 mmol N2 fixed/gram dry weight cell/hour）. Inoculation of the four best isolates onto Robusta coffee seedling positively enhanced the seedling growth in this green house experiment. Based on the results of Becton Dickinson＇s （BD） PhoenixTM Automated Microbiology System biochemical tests, Asm Bls.l isolates has similarities with Achromobacter sp., Asm E6s.l and Asm E6s.3.a had similarities with Stenotrophomonas maltophilia, while Ab Kws. 1 had similarities with Leifsonia aquatica.
文摘星巴克综合出品质量、服务水平、装修环境和配套设施4个方面的评价较高。在消费者最为看重的'出品质量'上,猫屎表现较佳,而COSTA和太平洋相对较差。到咖啡店,点杯咖啡坐下来,或发呆,或会务,或交友。面对越来越多的咖啡馆,消费者如何在喜爱的咖啡环境中喝到满意的咖啡?本刊此次送检星巴克、COSTA、猫屎、太平洋、咖啡陪你、麦咖啡、Lavazza Solo
文摘Objective： To study the effect of puncturing points Taiyang （Ex-HN 5）, Jiaosun （TE 20）, Qiuxu （GB 40） and Shenmai （BL 62） on migraine. Methods： The 120 cases in the treatment group were treated with routine acupuncture therapy, while the 40 cases in the control group were treated with Ergotamine and Caffeine Tablet, and then the therapeutic effect and recovery time of the two groups were compared. Results： The recovery rate of the treatment group was 90.0%, which is higher than 70.0% of the control group （P〈 0.01）. The total effective rate of the treatment group was 100%, which is better than 92.5% of the control group （P 〈 0.05）, The therapeutic effect of the treatment group was significantly better than the control group and the treatment group needed shorter treatment period （P 〈 0.05）. Conclusions： Acupuncture has higher recovery rate and total effective rate as well as shorter treatment period than oral ergotamine and caffeine tablets, which indicated that acupuncture is superior to the routinely-administered oral western tablets in treating migraine.
