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小麦珠心细胞衰退过程中ATP酶的超微细胞化学定位 被引量:15
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作者 田国伟 申家恒 《Acta Botanica Sinica》 CSCD 1996年第2期100-104,共5页
采用磷酸铅沉淀技术对小麦(Triticum aestivum )珠心细胞衰退过程进行了ATP酶的超微细胞化学定位。初始衰退的珠心细胞,ATP酶只定位于细胞膜上,其它部位未见有ATP酶活性。衰退中期的珠心细胞,细胞膜上A... 采用磷酸铅沉淀技术对小麦(Triticum aestivum )珠心细胞衰退过程进行了ATP酶的超微细胞化学定位。初始衰退的珠心细胞,ATP酶只定位于细胞膜上,其它部位未见有ATP酶活性。衰退中期的珠心细胞,细胞膜上ATP酶活性减弱并逐渐消失;细胞核染色质和细胞质中一些细胞器上存在ATP酶活性。在严重衰退的珠心细胞中,只在细胞核染色质上存在ATP酶活性。珠心细胞的细胞核以两种方式衰退。衰退的细胞核染色质碎片仍存在ATP酶活性,并向胚囊方向转移。推测小麦珠心细胞衰退过程中细胞膜上ATP酶变化反映了珠心细胞生理状态转变; 展开更多
关键词 麦心细胞 衰退 腺苷三磷酸酶 超微细化学
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Ultracytochemical Localization of Acid Phosphatase in Nucellar Cells of Wheat During Degeneration 被引量:6
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作者 田国伟 申家恒 尤瑞麟 《Acta Botanica Sinica》 CSCD 1999年第8期791-794,共4页
The distribution of acid phosphatase activity in nucellar cells of wheat ( Triticum aestivum L.) during degeneration has been studied using the lead precipitation method at the electron microscopic level. Acid phos... The distribution of acid phosphatase activity in nucellar cells of wheat ( Triticum aestivum L.) during degeneration has been studied using the lead precipitation method at the electron microscopic level. Acid phosphatase was localized in the slightly condensed nuclear chromatin in nucellar cells without any sign of ultrastructural degeneration. As the nucellar cells started degenerating, the enzyme activity in the cell was observed, in the order from small vacuoles to cell walls, mitochondria, plastids and endoplasmic reticulum. Enzyme activity was the highest in most components of the nucellar cells adjacent to the embryo sac where the degeneration of nucellar cells was the strongest, but it was not observed in the nuclei of the degenerated nucellar cells. The results indicated that the degeneration of nucellar cells was a progressive and orderly process and supported that the degeneration of nucellar cells was a programmed cell death. 展开更多
关键词 Triticum aestivum Nucellar cell DEGENERATION Acid phosphatase Ultracytochemical localization
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