The low-molecular-weight glutenin subunits(LMW-GS)account for 40% of gross glutenins of wheat,usually used as a major indicator for wheat quality improvement.Here,I comprehensively reviewed the advances in LMW-GS fr...The low-molecular-weight glutenin subunits(LMW-GS)account for 40% of gross glutenins of wheat,usually used as a major indicator for wheat quality improvement.Here,I comprehensively reviewed the advances in LMW-GS from their classification,coding gene loci on chromosomes and structural features,polypeptide chains composition,gene sequences accessed in Genbank,molecular markers saturation level,and their impacts on wheat process quality,in order to provide a theoretical basis for the utilization of LMW-GS in genetic improvement of wheat quality.展开更多
The high-molecular-weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU, 2n = 2x = 14) were characterized using SDS-PAGE analysis and molecular approaches. SDS-PAGE analysis showed...The high-molecular-weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU, 2n = 2x = 14) were characterized using SDS-PAGE analysis and molecular approaches. SDS-PAGE analysis showed that the 1Ux subunits from four different accessions possessed electrophoretic mobilities close to, or slower than, that displayed by the 1Dx2.2 subunit of common wheat. The electrophoretic mobilities of the 1Uy subunits were generally similar to those shown by the 1Dy subunits of common wheat. The complete open reading frames of the 1Ux and 1Uy genes were amplified by PCR and subsequently cloned and sequenced. Amino acid sequence comparisons suggested that the primary structure of the 1Ux and 1Uy subunits were identical to that of published HMW glutenin subunits from related species, Phylogenetic analysis indicated that the HMW glutenin subunits of Ae. umbellulata were most closely related to those encoded by the D genome of Triticeae.展开更多
文摘The low-molecular-weight glutenin subunits(LMW-GS)account for 40% of gross glutenins of wheat,usually used as a major indicator for wheat quality improvement.Here,I comprehensively reviewed the advances in LMW-GS from their classification,coding gene loci on chromosomes and structural features,polypeptide chains composition,gene sequences accessed in Genbank,molecular markers saturation level,and their impacts on wheat process quality,in order to provide a theoretical basis for the utilization of LMW-GS in genetic improvement of wheat quality.
文摘The high-molecular-weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU, 2n = 2x = 14) were characterized using SDS-PAGE analysis and molecular approaches. SDS-PAGE analysis showed that the 1Ux subunits from four different accessions possessed electrophoretic mobilities close to, or slower than, that displayed by the 1Dx2.2 subunit of common wheat. The electrophoretic mobilities of the 1Uy subunits were generally similar to those shown by the 1Dy subunits of common wheat. The complete open reading frames of the 1Ux and 1Uy genes were amplified by PCR and subsequently cloned and sequenced. Amino acid sequence comparisons suggested that the primary structure of the 1Ux and 1Uy subunits were identical to that of published HMW glutenin subunits from related species, Phylogenetic analysis indicated that the HMW glutenin subunits of Ae. umbellulata were most closely related to those encoded by the D genome of Triticeae.