麻栎叶黄酮的提取及其抗氧化活性研究

在单因素试验的基础上,对麻栎叶黄酮提取工艺进行优化,并研究了麻栎叶黄酮类化合物的抗氧化活性。结果显示,麻栎叶黄酮提取的最佳工艺是以60%乙醇为提取剂,提取物固液比1∶20,提取温度60℃,提取次数2次,微波功率640 W、微波时间2 min,提取时间60 min,黄酮提取率为6.06 mg/g,回收率为96.5%。麻栎叶黄酮类化合物对自由基DPPH·、·OH以及亚硝酸盐的清除率分别达到97.7%、97.6%和94.4%。试验结果表明提取工艺合理,黄酮提取率高,麻栎叶黄酮提取液具有很好的抗氧化活性。

麻栎叶黄酮的大孔树脂分离及其对α-葡萄糖苷酶活性的抑制作用

研究麻栎叶黄酮的大孔吸附树脂分离纯化工艺,并考察其对α-葡萄糖苷酶活性的抑制作用。通过大孔树脂静态吸附动力学实验,在确定大孔树脂类型的基础上,探索其最佳纯化工艺,结果表明,X-5是适用于麻栎叶黄酮吸附分离的较理想的树脂类型。X-5大孔吸附树脂分离纯化麻栎叶黄酮的最佳条件为:上柱液浓度52.79μg/mL左右,上柱液量为50mL,上柱液流速为0.5mL/min,上柱液pH为5。用95%乙醇进行洗脱,洗脱液流速为1.0mL/min,洗脱液量为30mL。在上述最佳条件下,X-5大孔吸附树脂分离纯化麻栎叶黄酮的纯度达58.33%。麻栎叶黄酮具有很强的抑制α-葡萄糖苷酶活性,IC为13.11μg/mL。

麻栎叶总黄酮提取工艺研究

采用微波提取麻栎叶总黄酮,设计正交试验考察提取液浓度、提取时间和固液比对提取率的影响,结果表明,乙醇浓度和提取时间对麻栎叶总黄酮提取率有显著的影响((0.01<P<0.05)),而固液比对提取率影响不显著。麻栎叶总黄酮最佳提取工艺为乙醇浓度60%,提取时间为每次60min,固液比为1:20。

Regression Relationship between WI and FMC at Different Growth Periods of Sawtooth Oaks Leaf

[Objective] The aim was to study the regression relationship between water index （WI） and fuel moisture content （FMC） of different growth periods of sawtooth oaks leaf.[Method] Taking sawtooth oaks in Huaguo Mountain,Lianyungang City as research object,the sensitivity of WI to leaf FMC was studied at leaf level,and statistical characteristics were analyzed.[Result] The WI of sawtooth oaks leaves was sensitive to the changes of FMC,and the line regression level between them was significant.A fitting curve between leaf FMC and WI was obtained.[Conclusion] The research provides reference for acquisition methods of vegetation water remote sensing within the range of study area.