采用软件Primer premier 5设计引物,从麻类脱胶高效菌株DCE01中克隆出一个果胶酶基因Pel325,重组到表达载体p ET-28a中,在E.coli BL21(DE3)中成功表达。试验结果显示,酶的活性随着底物(橘子果胶)酯化程度的增加而增加。胞内酶用酯化程度...采用软件Primer premier 5设计引物,从麻类脱胶高效菌株DCE01中克隆出一个果胶酶基因Pel325,重组到表达载体p ET-28a中,在E.coli BL21(DE3)中成功表达。试验结果显示,酶的活性随着底物(橘子果胶)酯化程度的增加而增加。胞内酶用酯化程度≥85%的橘子果胶作底物检测时酶活最高(酶活为37.5U/ml),其最适反应温度为55℃,最适反应p H为8.0。该结果可为进一步发掘DCE01菌株的基因资源提供重要科学依据。展开更多
The biochemical degumming and chemical degumming were employed to process apocynum venetum, and indices values for the residual gum content, fineness, strength of single fiber, average length, moisture regain and whit...The biochemical degumming and chemical degumming were employed to process apocynum venetum, and indices values for the residual gum content, fineness, strength of single fiber, average length, moisture regain and whiteness of degummed apocynum venetum fibers are evaluated with fuzzy mathematical method. The results obtained from this investigation show that the general index value of degummed apocynum venetum fibers with biochemical degumming (K1= 0.626 09) is prior to that with chemical degumming(K2=0.421 64).展开更多
文摘The biochemical degumming and chemical degumming were employed to process apocynum venetum, and indices values for the residual gum content, fineness, strength of single fiber, average length, moisture regain and whiteness of degummed apocynum venetum fibers are evaluated with fuzzy mathematical method. The results obtained from this investigation show that the general index value of degummed apocynum venetum fibers with biochemical degumming (K1= 0.626 09) is prior to that with chemical degumming(K2=0.421 64).