持久黄体性不孕症患牛电疗前后外周血清中孕酮和17β－雌二醇的变化

持久黄体性不孕症患牛电疗前后外周血清中孕酮和１７β－雌二醇的变化梁俭，马继东，杨蔚祯，李汝舟曾国庆，马魁榕，赵恒元（宁夏畜牧兽医研究所７５０００２）（中国科学院动物研究所）孙成彦，周兴伟，王少金（宁夏平吉堡奶牛场）为进一步了解电疗对某些生殖激素的影响...

中医药治疗黄体性不孕症98例临床报导

本文分别运用补肾健脾,滋阴清热,疏肝解郁,活血化瘀的治法,配合周期疗法,治疗98例黄体功能不全患者,达到妊娠者81例。

Photocatalytic activity and kinetics for acid yellow degradation over surface composites of TiO_2-coated activated carbon under different photocatalytic conditions

TiO2-coated activated carbon surface （TAs） composites were prepared by a sol-gel method with supercritical pretreatment. The photocatalytic degradation of acid yellow （AY） was investigated under UV radiation to estimate activity of catalysts and determine the kinetics. And the effects of parameters including the initial concentration of AY, light intensity and TiO2 content in catalysts were examined. The results indicate that TAs has a higher efficiency in decomposition of AY than P25, pure TiO2 particles as well as the mixture of TiO2 powder and active carbon. The photocatalytic degradation rate is found to follow the pseudo-first order kinetics with respect to the AY concentration. The new kinetic model fairly resembles the classic Langmuir-Hinshelwood equation, and the rate constant is proportional to the square root of the light intensity in a wide range. However, its absorption performance depends on the surface areas of catalysts. The model fits quite well with the experimental data and elucidates phenomena about the effects of the TiO2 content in TAs on the degradation rate.

中西医结合治疗奶牛生殖疾病

1 子宫积脓(积水) 多数采用子宫冲洗加肌注或灌注麦角新碱、催产素等促子宫收缩类药物的治疗方案，但此法工作量大、持续时间长，常治疗不彻底。我们采用中药粉拌料口服法，疗效显著，治愈率高，且简单实用。……

Preparation, Stability and Immunoenhancement of APS (Astragalus polysaccharide) Liposomes

Six factors and 10 levels of each factor were selected by using the (uniform design method( with the aid of the computer for preparing APS liposomes. The optimal procedure for preparing APS liposomes was established and it can suit the large scale production in a pharmaceutical factory. The shelf-life of APS liposomes at 20℃ is 1.46 years. Diameters of the vesicles ( > 90% ) in APS liposomes are less than 1 μm, and the system is stable. At 40℃ the diameters of vesicles were not changed in three months. Pharmacological experiments revealed that APS liposomes exerted a strong immunoenhancement in mice. Studies in this paper established a foundation for the production and the clinical application of APS liposomes.

秋风送爽桂花香

＂暗淡轻黄体性柔,情疏迹远只香留,何须浅碧深红色,自是花中第一流。梅定妒,菊应羞,画栏开处冠中秋,骚人可煞无情思,何事当年不见收。＂这是宋代女词人李清照写桂花的一首词。词中抓住桂花的特征,描绘了桂花虽轻盈柔弱,但它的气味芳香浓郁。

Ontogeny and Metamorphic Patterns of Female Reproductive Organs of Ephedra sinica Stapf (Ephedraceae)

Ontogeny and metamorphic. development of female reproductive organs in Ephedra sinica Stapf were surveyed. At the end of April, female cones began to initialize from the vegetative buds. Pollination occurred in mid-May and seeds matured at the beginning of July. The ontogenetic pattern of female reproductive organs of E. sin ca is basically similar to that of E. distachya L. The foliar nature of the outer envelope of the ovule in Ephedra is corroborated. Reduction of ovule number as a tendency of speciation in the genus is substantiated by the occurrence of tri-ovulate cones coupled with comprehensive characters of the genus. The metamorphic patterns as well as the leaf nature of the outer envelope indicate that female cones of Ephedra are compound while the female reproductive units of the ovulate cone have been reduced from secondary reproductive shoots similar to those of cordaites by phylogenetic transformation, fusion and reduction. Each fertile bract together with its axillary female reproductive unit composed the Bract Scale and Seed Scale Complex of Ephedra.

Tyrosinase,a new innate humoral immune parameter in large yellow croaker(Pseudosciaena crocea R)

We evaluated the immune response to infection with a pathogen in large yellow croaker(Pseudosciaena crocea Richardson).The fish were given an intraperitoneal(i.p.) injection of Vibrio parahaemolyticus or sterile sea water(control).We collected blood sera from the fish 0.17,1,2,4,8,12,or 16 d after injection(dpi).We measured tyrosinase activity and the concentrations of lysozyme,NOS,and antibodies.Serum tyrosinase activity was significantly higher at 0.17 and 4 dpi than in the control group,and peaked at 8 dpi.Lysozyme activity was significantly higher at 2 and 12 dpi than in the control group,but lower at 16 dpi.There is no statistical difference in the level of nitric oxides synthase(NOS) activity or antibodies between the control and injection groups.This is the first report of the tyrosinase activity in the serum of large yellow croaker.Our results indicate that tyrosinase plays an important role in the immediate immune defense against V.parahaemolyticus in large yellow croaker.Tyrosinase is a candidate parameter for investigation of fish innate immune defense.

Effect of dietary genistein on growth performance,digestive enzyme activity,and body composition of Nile tilapia Oreochromis niloticus

An 8-week feeding experiment was performed to evaluate the effect of dietary genistein on growth performance,body composition,and digestive enzymes activity of juvenile Nile tilapia(Oreochromis niloticus).Four isonitrogenous and isoenergetic diets were formulated containing four graded supplements of genistein:0,30,300,and 3 000 μg/g.Each diet was randomly assigned in triplicate to tanks stocked with 15 juvenile tilapia(10.47±1.24 g).The results show that 30 and 300 μg/gdietary genistein had no significant effect on growth performance of Nile tilapia,but the higher level of genistein(3 000 μg/g)significantly depressed the final body weight and specific growth rate.There was no significant difference in survival rate,feed intake,feed efficiency ratio or whole body composition among all dietary treatments.An assay of digestive enzymes showed that the diet containing 3 000 μg/ggenistein decreased stomach and hepatopancreas protease activity,and amylase activity in the liver and intestine,while a dietary level of300 μg/g genistein depressed stomach protease and intestine amylase activities.However,no significant difference in stomach amylase activity was found among dietary treatments.Overall,the results of the present study indicate that a high level of dietary genistein(3 000 μg/g,or above) would significantly reduce the growth of Nile tilapia,partly because of its inhibitory effect on the activity of major digestive enzymes.Accordingly,the detrimental effects of genistein,as found in soybean products,should not be ignored when applied as an alternative ingredient source in aquaculture.

Artificial neural network modeling of gold dissolution in cyanide media

The effects of cyanidation conditions on gold dissolution were studied by artificial neural network （ANN） modeling. Eighty-five datasets were used to estimate the gold dissolution. Six input parameters, time, solid percentage, P50 of particle, NaCN content in cyanide media, temperature of solution and pH value were used. For selecting the best model, the outputs of models were compared with measured data. A fourth-layer ANN is found to be optimum with architecture of twenty, fifteen, ten and five neurons in the first, second, third and fourth hidden layers, respectively, and one neuron in output layer. The results of artificial neural network show that the square correlation coefficients （R2） of training, testing and validating data achieve 0.999 1, 0.996 4 and 0.9981, respectively. Sensitivity analysis shows that the highest and lowest effects on the gold dissolution rise from time and pH, respectively It is verified that the predicted values of ANN coincide well with the experimental results.

Plasma electrolytic oxidation behavior and corrosion resistance of brass in aluminate electrolyte containing NaH2PO4 or Na2SiO3

Plasma electrolytic oxidation(PEO) of brass was carried out in aluminate electrolytes with the addition of NaH2PO4(S1) and Na2SiO3(S2), respectively, with the aim to investigate the effect of additives on the coating formation and corrosion resistance. For the PEO in S1 electrolyte, a mixed layer of AlPO4and Al2O3is formed at the initial stage, which leads to fast plasma discharges and formation of black coatings with the compositions of Al2O3,CuO, Cu2O and ZnO. However, in S2 electrolyte, plasma discharges are delayed and the coatings show a reddish color due to more Cu2O. Mott-Schottky tests show that the S1 coatings are p-type semiconductors;while the S2 coatings can be adjusted between n-type and p-type. Potentiodynamic polarization and electrochemical impedance spectroscopy(EIS) tests show that the PEO treatment can significantly improve the corrosion resistance of brass, with protection efficiency up to 91.50% and the largest charge transfer resistance of 59.95 kΩ·cm^(2) for the S1 coating.

Estrogen and progesterone receptor isoforms expression in the stomach of Mongolian gerbils

AIM: We studied the estrogen receptor (ER) and progesterone receptor (PR) isoforms expression in gastric antrum and corpus of female gerbils and their regulation by estradiol (E2) and progesterone (P4). METHODS: Ovariectomized adult female gerbils were subcutaneously treated with E2, and E2 + P4. Uteri and stomachs were removed, the latter were cut along the greater curvature, and antrum and corpus were excised. Proteins were immunoblotted using antibodies that recognize ER-alpha, ER-beta, and PR-A and PR-B receptor isoforms. Tissues from rats treated in the same way were used as controls. RESULTS: Specific bands were detected for ER-alpha (68 KDa), and PR isoforms (85 and 120 KDa for PR-A and PR-B isoforms, respectively) in uteri, gastric antrum and corpus. We could not detect ER-beta isoform. PR isoforms were not regulated by E2 or P4 in uterus and gastric tissues of gerbils. ER-alpha isoform content was significantly down-regulated by E2 in the corpus, but not affected by hormones in uterus and gastric antrum. CONCLUSION: The presence of ER-alpha and PR isoforms in gerbils stomach suggests that E2 and P4 actions in this organ are in part mediated by their nuclear receptors.

Study on the Control of Tobacco Black Shank by Using Dry Mycelium of Penicillium Chrysogenum

The water extract of dry mycelium ofPenicillium chrysogenum （DME） was used to induce resistance in Virginia tobacco plants against Phtophthora parasitica var. nicotianae. Results showed that the efficacy of DME in controlling black shank disease depended on both DME solution concentration and its＇ treatment methodology. Soil application of 1.5-5% DME 72 hr before inoculation with Phtophthora parasitica vat. nicotianae provided highly significant protection against black shank, relative to the control without DME treatment. Optimized tobacco plant treatment with 2.5% DME significantly increased peroxidase （POD） and polyphenol oxidase （PPO） activity levels in the upper leaf sections of the tobacco plants. DME had no direct antifungal activity on the growth of Phtophthora parasitica var. nicotianae in vitro, suggesting that disease control with DME treatment resulted from the induced propagation of natural defense mechanisms in the tobacco plants.

Central administration of Orphanin FQ inhibits GnRH secretion by ORL1 receptor in the median eminence of freely moving ovariectomized rats

Objective This study aimed to investigate the possible role of Orphanin FQ(OFQ) in the regulation of hypothalamic gonadotropin-releasing hormone(GnRH) secretion. Methods The method of push-pull perfusion and radioimmunoassay(RIA) were adopted to examine the secretory profile of GnRH in the median eminence(ME) in freely moving ovariectomized(OVX) rats after intracerebroventricular(icv) injection of OFQ and/or [Nphe1]NC(1-13)NH2(NC13),a competitive antagonists of the opioid receptor-like 1 receptor(ORL1 recep...

Effect of Acupuncture on Infertility Due to Luteal Phase Defect

Objective：To observe the effect of acupuncture for infertility due to luteal phase defect （LPD）.Method：Fifty cases were randomized into an acupuncture group （30 cases） and a medication （Clomiphene） group （20 cases）.High phase score （HPS） was adopted to evaluate the luteal function before and after treatments,coupled with observation on the changes of E 2,progesterone （P）,follicles and endometrium.Results：The total effective rate in the acupuncture group was 93.4%,versus 70.0% in the medication group,showing a significant difference （P〈0.05）.Conclusion：Both acupuncture and Clomiphene can substantially improve the luteal function;however,acupuncture can get a better effect in improving the endometrium and a lower miscarriage rate than Clomiphene.

Synthesis, properties and applications of one- and two- dimensional gold nanostructures

The controlled synthesis of gold nanocrystals has been the subject of intensive studies for decades because the properties and functions of gold nanomaterials are highly dependent on their particle size, shape, and dimensionality. Especially, anisotropic gold nanocrystals, such as nanowires, nanobelts, nanoplates and nanosheets, have attracted much attention due to their striking properties and promising applications in electronics, catalysis, photonics, sensing and biomedicine. In this review, we will summarize the recent developments of one- dimensional （1D） and two-dimensional （2D） gold nanostructures. Various kinds of synthetic methods for preparation of these 1D and 2D gold nanocrystals will be described. Moreover, we will also briefly introduce the properties and potential applications of these 1D and 2D gold nanocrystals.