AIM： To evaluate the utility of local tumor therapy combined with percutaneous transhepatic bilian/drainage （PTBD） for malignant obstructive bilian/disease. METHODS： A total of 233 patients with malignant billan/o...AIM： To evaluate the utility of local tumor therapy combined with percutaneous transhepatic bilian/drainage （PTBD） for malignant obstructive bilian/disease. METHODS： A total of 233 patients with malignant billan/obstruction were treated in our hospital with PTBD by placement of metallic stents and/or plastic tubes. After PTBD, 49 patients accepted brachytherapy or extraradiation therapy or arterial infusion chemotherapy. The patients were followed up with clinical and radiographic evaluation. The survival and stent patency rate were calculated by Kaplan-Meier survival analysis. RESULTS： Twenty-two patients underwent chemotherapy （11 cases of hepatic carcinoma, 7 cases of pancreatic carcinoma, 4 cases of metastatic lymphadenopathy）, and 14 patients received radiotherapy （10 cases of cholangiocardnoma, 4 cases of pancreatic carcinoma）, and 13 patients accepted brachytherapy （7 cases of cholangiocarcinoma, 3 cases of pancreatic carcinoma, 4 cases of metastatic lymphadenopathy）. The survival rate of the local tumor treatment group at 1, 3, 6, and 12 months was 97.96%, 95.92%, 89.80%, and 32.59% respectively, longer than that of the non treatment group. The patency rate at 1, 3, 6, and 12 months was 97.96%, 93.86%, 80.93%, and 56.52% respectively. The difference of patency rate was not significant between treatment group and non treatment group. CONCLUSION： Our results suggest that local tumor therapy could prolong the survival time of patients with malignant biliary obstruction, and may improve stent patency.展开更多
A study was conducted to test the influence of different inorganic fertilizers （phosphorous and potassium） on the nodulation and growth of Dalbergia sissoo grown in the nursery. Before seeds sowing, different combin...A study was conducted to test the influence of different inorganic fertilizers （phosphorous and potassium） on the nodulation and growth of Dalbergia sissoo grown in the nursery. Before seeds sowing, different combinations of P, K fertilizers were incorporated with the nutrient-deficient natural forest soils, and then amended with cowdung （soil： cowdung = 3：1）. Nodulation status （nodule number, shape, fresh weight, dry weight and color） in the roots and the plant growth parameters （length of shoot and root, collar diameter, fresh and dry weight of shoot and root） were recorded 60 days after seeds sowing. Nodulation status and growth of the plants varied significantly （P〈0.05） in the soils amended with fertilizers in comparison to the control. The highest nodule number （62）, fresh （0.50 g） and dry （0.07 g） weights were recorded with the dose of PK at the rate of 160 kg/hm2. Nodule shape and color also varied widely in different treatments. In case of plant growth parameters, shoot and root length, collar diameter, fresh and dry weight of the plants took on a significant difference （P〈0.05） among various combination of fertilizers. From the study, it is revealed that PK at the rate of 160 kg/hm^2 fertilizer with soil and cowdung mixture （soil： eowdung = 3：1） is recommended for optimum growth and nodule formation of D. sissoo in degraded soils at a nursery level.展开更多
Survey of yellowfin tuna in the west-central Indian Ocean was conducted on board of Chinese longliners during 2003,2004 and 2005,which is a part of Chinese Tuna Fishery Scientific Observer Program(CTFSOP) . The reprod...Survey of yellowfin tuna in the west-central Indian Ocean was conducted on board of Chinese longliners during 2003,2004 and 2005,which is a part of Chinese Tuna Fishery Scientific Observer Program(CTFSOP) . The reproductive biology has been investigated. A total of 1 023 samples are collected including 417 ovaries and 606 testes. Spawning activities of yellowfin tuna have been studied for both male and female from January to June. The data showed that the average monthly sex ratio is 0.59,and the minimum length at sexual maturity is 101 cm for female and 110 cm for male respectively. Length at 50% sexual maturity is esti-mated at 113.77 cm for female and 120.20 cm for male,whereas maturation rate is 0.066 cm-1 for female and 0.091 cm-1 for male. Sex ratio by length class indicates that the proportion of male is higher than female's along with size increasing;for instance,in the group of the body length longer than 145 cm,some females have their body length from 145 to 160 cm and males have their body length at 160 cm and even longer. Statistically,yellowfin tuna has a significant seasonal reproduction.展开更多
AIM:To determine the effects of curcumin,(-)-epigallocatechin-3-gallate (EGCG),lovastatin,and their combinations on inhibition of esophageal cancer.METHODS:Esophageal cancer TE-8 and SKGT-4 cell lines were subjected t...AIM:To determine the effects of curcumin,(-)-epigallocatechin-3-gallate (EGCG),lovastatin,and their combinations on inhibition of esophageal cancer.METHODS:Esophageal cancer TE-8 and SKGT-4 cell lines were subjected to cell viability methyl thiazolyl tetrazolium and tumor cell invasion assays in vitro and tumor formation and growth in nude mouse xenografts with or without curcumin,EGCG and lovastatin treatment.Gene expression was detected using immunohistochemistry and Western blotting in tumor cell lines,tumor xenografts and human esophageal cancer tissues,respectively.RESULTS:These drugs individually or in combinations significantly reduced the viability and invasion capacity of esophageal cancer cells in vitro.Molecularly,these three agents reduced the expression of phosphorylated extracellular-signal-regulated kinases (Erk1/2),c-Jun and cyclooxygenase-2 (COX-2),but activated caspase 3 in esophageal cancer cells.The nude mouse xenograft assay showed that EGCG and the combinations of curcumin,EGCG and lovastatin suppressed esophageal cancer cell growth and reduced the expression of Ki67,phosphorylated Erk1/2 and COX-2.The expression of phosphorylated Erk1/2 and COX-2 in esophageal cancer tissue specimens was also analyzed using immunohistochemistry.The data demonstrated that 77 of 156 (49.4%) tumors expressed phosphorylated Erk1/2 and that 121 of 156 (77.6%) esophageal cancers expressed COX-2 protein.In particular,phosphorylated Erk1/2 was expressed in 23 of 50 (46%) cases of esophageal squamous cell carcinoma (SCC) and in 54 of 106 (50.9%) cases of adenocarcinoma,while COX-2 was expressed in 39 of 50 (78%) esophageal SCC and in 82 of 106 (77.4%) esophageal adenocarcinoma.CONCLUSION:The combinations of curcumin,EGCG and lovastatin were able to suppress esophageal cancer cell growth in vitro and in nude mouse xenografts,these drugs also inhibited phosphorylated Erk1/2,c-Jun and COX-2 expression.展开更多
Resulting from the collision of the Eurasian and Indian plates, the Qinghai-Tibetan Plateau is commonly known as the ＇roof of the world＇. Collectively the Yarlung Tsangpo, Nu, Laneang, Yangtze, Yalong, and Yellow Ri...Resulting from the collision of the Eurasian and Indian plates, the Qinghai-Tibetan Plateau is commonly known as the ＇roof of the world＇. Collectively the Yarlung Tsangpo, Nu, Laneang, Yangtze, Yalong, and Yellow River basins drain the eastern margin of the plateau. In this paper, we utilize Shuttle Radar Topography Mission elevation data to examine morphometric and relief attributes of these basins to reveal insights rates of incision. A robust into tectonic activity and technique using Maflab is proposed to alleviate errors associated with SRTM data in the derivation of river longitudinal profiles. Convex longitudinal profiles are interpreted to be a product of uplift rates that exceed rates of channel incision along the entire margin of the Qinghai- Tibetan Plateau. Highest relief towards the south reflects extensive fluvial incision. High relief is also prominent along major active faults. Erosion patterns are related to distance from knickpoints. Highest rates of erosion and incision are evident towards the south, with decreasing values towards the north, suggesting a link between tectonic activity and erosion.展开更多
AIM:To investigate the adjunct anticancer effect of Astragalus polysaccharides in H22 tumor-bearing mice.METHODS:To establish a solid tumor model,5.0 × 10 6 /mL H22 hepatoma cells were inoculated subcutaneously i...AIM:To investigate the adjunct anticancer effect of Astragalus polysaccharides in H22 tumor-bearing mice.METHODS:To establish a solid tumor model,5.0 × 10 6 /mL H22 hepatoma cells were inoculated subcutaneously into the right armpit region of Kunming mice(6-12 wk old,18-22 g).When the tumors reached a size of 100 mm 3,the animals were treated as indicated,and the mice were randomly assigned to seven groups(n = 10 each).After ten days of treatment,blood samples were collected from mouse eyes,and serum was harvested by centrifugation.Mice were sacrificed,and the whole body,tumor,spleen and thymus were weighed immediately.The rate of tumor inhibition and organ indexes were calculated.The expression levels of serum cytokines,P-glycoprotein(P-GP) and multidrug resistance(MDR) 1 mRNA in tumor tissues were detected using enzyme-linked immunosorbent assay,Western blotting,and quantitative myeloid-derived suppressor cells reverse transcription-polymerase chain reaction,respectively.RESULTS:The tumor inhibition rates in the treatment groups of Adriamycin(ADM) + Astragalus polysaccharides(APS)(50 mg/kg),ADM + APS(100 mg/kg),and ADM + APS(200 mg/kg) were significantly higher than in the ADM group(72.88% vs 60.36%,P = 0.013;73.40% vs 60.36%,P = 0.010;77.57% vs 60.36%,P = 0.001).The spleen indexes of the above groups were also significantly higher than in the ADM group(0.65 ± 0.22 vs 0.39 ± 0.17,P = 0.023;0.62 ± 0.34 vs 0.39 ± 0.17,P = 0.022;0.67 ± 0.20 vs 0.39 ± 0.17,P = 0.012),and the thymus indexes of the ADM + APS(100 mg/kg) and ADM + APS(200 mg/kg) groups were significantly higher than in the ADM group(0.20 ± 0.06 vs 0.13 ± 0.04,P = 0.029;0.47 ± 0.12 vs 0.13 ± 0.04,P = 0.000).APS was found to exert a synergistic antitumor effect with ADM and to alleviate the decrease in the sizes of the spleen and thymus induced by AMD.The expression of interleukin-1α(IL-1α),IL-2,IL-6,and tumor necrosis factor-α(TNF-α) was significantly higher in the ADM + APS(50 mg/kg),ADM + APS(100 mg/kg) and ADM + APS(200 mg/kg) groups than in the ADM group;and IL-10 was significantly lower in the above groups than in the ADM group.APS could increase IL-1α,IL-2,IL-6,and TNF-α expression and decrease IL-10 levels.Compared with the ADM group,APS treatment at a dose of 50-200 mg/kg could downregulate MDR1 mRNA expression in a dose-dependent manner(0.48 ± 0.13 vs 4.26 ± 1.51,P = 0.000;0.36 ± 0.03 vs 4.26 ± 1.51,P = 0.000;0.21 ± 0.04 vs 4.26 ± 1.51,P = 0.000).The expression level of P-GP was significantly lower in the ADM + APS(200 mg/kg) group than in the ADM group(137.35 ± 9.20 mg/kg vs 282.19 ± 20.54 mg/kg,P = 0.023).CONCLUSION:APS exerts a synergistic anti-tumor effect with ADM in H22 tumor-bearing mice.This may be related to its ability to enhance the expression of IL1α,IL-2,IL-6,and TNF-α,decrease IL-10,and downregulate MDR1 mRNA and P-GP expression levels.展开更多
To find the method for the integrated control of Tetranychus cinnabarinus （carmine spider mite） and delay its resistance to pesticides, the joint actions of each of five commonly pesticides （abamectin, azadirachtin...To find the method for the integrated control of Tetranychus cinnabarinus （carmine spider mite） and delay its resistance to pesticides, the joint actions of each of five commonly pesticides （abamectin, azadirachtin, matrine, pyrethrins and imidacloprid）, and Neoseiulus cucumeris （Oudemans）, the natural enemy of cotton red spider mite, were studied. N. cucumeris was released after application of pesticides for seven days （for 0,3% azadirachtin EC） or six days （for other pesticides）. The results showed that the combined action of 1.8% abamectin EW （1：8 000） and N. cucumeris had the best control efficacy of 96.63% at 20 days after N. cucumeris releasing. The control efficacies of N. cucumeds and 0.3% azadirachtin EC （1：250） were 59.7% and 90.1% after one day and 20 days, respectively, after N. cucumeris releasing. The control efficacy of N. cucumeris and 0.5% matrine AS （1：2 000） was 82.65% at 20 days after N. cucumeris releasing. The results provide options for sustainable control of T. cinnabarinus and for the delay of pesticides resistance.展开更多
基金Supported by the Natural Science Foundation of Beijing, No.7982035
文摘AIM： To evaluate the utility of local tumor therapy combined with percutaneous transhepatic bilian/drainage （PTBD） for malignant obstructive bilian/disease. METHODS： A total of 233 patients with malignant billan/obstruction were treated in our hospital with PTBD by placement of metallic stents and/or plastic tubes. After PTBD, 49 patients accepted brachytherapy or extraradiation therapy or arterial infusion chemotherapy. The patients were followed up with clinical and radiographic evaluation. The survival and stent patency rate were calculated by Kaplan-Meier survival analysis. RESULTS： Twenty-two patients underwent chemotherapy （11 cases of hepatic carcinoma, 7 cases of pancreatic carcinoma, 4 cases of metastatic lymphadenopathy）, and 14 patients received radiotherapy （10 cases of cholangiocardnoma, 4 cases of pancreatic carcinoma）, and 13 patients accepted brachytherapy （7 cases of cholangiocarcinoma, 3 cases of pancreatic carcinoma, 4 cases of metastatic lymphadenopathy）. The survival rate of the local tumor treatment group at 1, 3, 6, and 12 months was 97.96%, 95.92%, 89.80%, and 32.59% respectively, longer than that of the non treatment group. The patency rate at 1, 3, 6, and 12 months was 97.96%, 93.86%, 80.93%, and 56.52% respectively. The difference of patency rate was not significant between treatment group and non treatment group. CONCLUSION： Our results suggest that local tumor therapy could prolong the survival time of patients with malignant biliary obstruction, and may improve stent patency.
文摘A study was conducted to test the influence of different inorganic fertilizers （phosphorous and potassium） on the nodulation and growth of Dalbergia sissoo grown in the nursery. Before seeds sowing, different combinations of P, K fertilizers were incorporated with the nutrient-deficient natural forest soils, and then amended with cowdung （soil： cowdung = 3：1）. Nodulation status （nodule number, shape, fresh weight, dry weight and color） in the roots and the plant growth parameters （length of shoot and root, collar diameter, fresh and dry weight of shoot and root） were recorded 60 days after seeds sowing. Nodulation status and growth of the plants varied significantly （P〈0.05） in the soils amended with fertilizers in comparison to the control. The highest nodule number （62）, fresh （0.50 g） and dry （0.07 g） weights were recorded with the dose of PK at the rate of 160 kg/hm2. Nodule shape and color also varied widely in different treatments. In case of plant growth parameters, shoot and root length, collar diameter, fresh and dry weight of the plants took on a significant difference （P〈0.05） among various combination of fertilizers. From the study, it is revealed that PK at the rate of 160 kg/hm^2 fertilizer with soil and cowdung mixture （soil： eowdung = 3：1） is recommended for optimum growth and nodule formation of D. sissoo in degraded soils at a nursery level.
文摘Survey of yellowfin tuna in the west-central Indian Ocean was conducted on board of Chinese longliners during 2003,2004 and 2005,which is a part of Chinese Tuna Fishery Scientific Observer Program(CTFSOP) . The reproductive biology has been investigated. A total of 1 023 samples are collected including 417 ovaries and 606 testes. Spawning activities of yellowfin tuna have been studied for both male and female from January to June. The data showed that the average monthly sex ratio is 0.59,and the minimum length at sexual maturity is 101 cm for female and 110 cm for male respectively. Length at 50% sexual maturity is esti-mated at 113.77 cm for female and 120.20 cm for male,whereas maturation rate is 0.066 cm-1 for female and 0.091 cm-1 for male. Sex ratio by length class indicates that the proportion of male is higher than female's along with size increasing;for instance,in the group of the body length longer than 145 cm,some females have their body length from 145 to 160 cm and males have their body length at 160 cm and even longer. Statistically,yellowfin tuna has a significant seasonal reproduction.
基金Supported by A United States National Cancer Institute Grant,No.R01CA117895a grant from the Duncan Family Institute for Cancer Prevention and Risk Assessment,UT MDAnderson Cancer Center
文摘AIM:To determine the effects of curcumin,(-)-epigallocatechin-3-gallate (EGCG),lovastatin,and their combinations on inhibition of esophageal cancer.METHODS:Esophageal cancer TE-8 and SKGT-4 cell lines were subjected to cell viability methyl thiazolyl tetrazolium and tumor cell invasion assays in vitro and tumor formation and growth in nude mouse xenografts with or without curcumin,EGCG and lovastatin treatment.Gene expression was detected using immunohistochemistry and Western blotting in tumor cell lines,tumor xenografts and human esophageal cancer tissues,respectively.RESULTS:These drugs individually or in combinations significantly reduced the viability and invasion capacity of esophageal cancer cells in vitro.Molecularly,these three agents reduced the expression of phosphorylated extracellular-signal-regulated kinases (Erk1/2),c-Jun and cyclooxygenase-2 (COX-2),but activated caspase 3 in esophageal cancer cells.The nude mouse xenograft assay showed that EGCG and the combinations of curcumin,EGCG and lovastatin suppressed esophageal cancer cell growth and reduced the expression of Ki67,phosphorylated Erk1/2 and COX-2.The expression of phosphorylated Erk1/2 and COX-2 in esophageal cancer tissue specimens was also analyzed using immunohistochemistry.The data demonstrated that 77 of 156 (49.4%) tumors expressed phosphorylated Erk1/2 and that 121 of 156 (77.6%) esophageal cancers expressed COX-2 protein.In particular,phosphorylated Erk1/2 was expressed in 23 of 50 (46%) cases of esophageal squamous cell carcinoma (SCC) and in 54 of 106 (50.9%) cases of adenocarcinoma,while COX-2 was expressed in 39 of 50 (78%) esophageal SCC and in 82 of 106 (77.4%) esophageal adenocarcinoma.CONCLUSION:The combinations of curcumin,EGCG and lovastatin were able to suppress esophageal cancer cell growth in vitro and in nude mouse xenografts,these drugs also inhibited phosphorylated Erk1/2,c-Jun and COX-2 expression.
基金Funding was provided by International Science&Technology Cooperation Program of China(Grant Nos.2011DFG93160,2011DFA20820)
文摘Resulting from the collision of the Eurasian and Indian plates, the Qinghai-Tibetan Plateau is commonly known as the ＇roof of the world＇. Collectively the Yarlung Tsangpo, Nu, Laneang, Yangtze, Yalong, and Yellow River basins drain the eastern margin of the plateau. In this paper, we utilize Shuttle Radar Topography Mission elevation data to examine morphometric and relief attributes of these basins to reveal insights rates of incision. A robust into tectonic activity and technique using Maflab is proposed to alleviate errors associated with SRTM data in the derivation of river longitudinal profiles. Convex longitudinal profiles are interpreted to be a product of uplift rates that exceed rates of channel incision along the entire margin of the Qinghai- Tibetan Plateau. Highest relief towards the south reflects extensive fluvial incision. High relief is also prominent along major active faults. Erosion patterns are related to distance from knickpoints. Highest rates of erosion and incision are evident towards the south, with decreasing values towards the north, suggesting a link between tectonic activity and erosion.
文摘AIM:To investigate the adjunct anticancer effect of Astragalus polysaccharides in H22 tumor-bearing mice.METHODS:To establish a solid tumor model,5.0 × 10 6 /mL H22 hepatoma cells were inoculated subcutaneously into the right armpit region of Kunming mice(6-12 wk old,18-22 g).When the tumors reached a size of 100 mm 3,the animals were treated as indicated,and the mice were randomly assigned to seven groups(n = 10 each).After ten days of treatment,blood samples were collected from mouse eyes,and serum was harvested by centrifugation.Mice were sacrificed,and the whole body,tumor,spleen and thymus were weighed immediately.The rate of tumor inhibition and organ indexes were calculated.The expression levels of serum cytokines,P-glycoprotein(P-GP) and multidrug resistance(MDR) 1 mRNA in tumor tissues were detected using enzyme-linked immunosorbent assay,Western blotting,and quantitative myeloid-derived suppressor cells reverse transcription-polymerase chain reaction,respectively.RESULTS:The tumor inhibition rates in the treatment groups of Adriamycin(ADM) + Astragalus polysaccharides(APS)(50 mg/kg),ADM + APS(100 mg/kg),and ADM + APS(200 mg/kg) were significantly higher than in the ADM group(72.88% vs 60.36%,P = 0.013;73.40% vs 60.36%,P = 0.010;77.57% vs 60.36%,P = 0.001).The spleen indexes of the above groups were also significantly higher than in the ADM group(0.65 ± 0.22 vs 0.39 ± 0.17,P = 0.023;0.62 ± 0.34 vs 0.39 ± 0.17,P = 0.022;0.67 ± 0.20 vs 0.39 ± 0.17,P = 0.012),and the thymus indexes of the ADM + APS(100 mg/kg) and ADM + APS(200 mg/kg) groups were significantly higher than in the ADM group(0.20 ± 0.06 vs 0.13 ± 0.04,P = 0.029;0.47 ± 0.12 vs 0.13 ± 0.04,P = 0.000).APS was found to exert a synergistic antitumor effect with ADM and to alleviate the decrease in the sizes of the spleen and thymus induced by AMD.The expression of interleukin-1α(IL-1α),IL-2,IL-6,and tumor necrosis factor-α(TNF-α) was significantly higher in the ADM + APS(50 mg/kg),ADM + APS(100 mg/kg) and ADM + APS(200 mg/kg) groups than in the ADM group;and IL-10 was significantly lower in the above groups than in the ADM group.APS could increase IL-1α,IL-2,IL-6,and TNF-α expression and decrease IL-10 levels.Compared with the ADM group,APS treatment at a dose of 50-200 mg/kg could downregulate MDR1 mRNA expression in a dose-dependent manner(0.48 ± 0.13 vs 4.26 ± 1.51,P = 0.000;0.36 ± 0.03 vs 4.26 ± 1.51,P = 0.000;0.21 ± 0.04 vs 4.26 ± 1.51,P = 0.000).The expression level of P-GP was significantly lower in the ADM + APS(200 mg/kg) group than in the ADM group(137.35 ± 9.20 mg/kg vs 282.19 ± 20.54 mg/kg,P = 0.023).CONCLUSION:APS exerts a synergistic anti-tumor effect with ADM in H22 tumor-bearing mice.This may be related to its ability to enhance the expression of IL1α,IL-2,IL-6,and TNF-α,decrease IL-10,and downregulate MDR1 mRNA and P-GP expression levels.
基金Supported by the Modern Agricultural Technology System of Hunan Province(Xiangnongyelian No.[2012]278)the Project of the Department of Science and Technology of Hunan Province(2010NK3011,2011FJ4298)~~
文摘To find the method for the integrated control of Tetranychus cinnabarinus （carmine spider mite） and delay its resistance to pesticides, the joint actions of each of five commonly pesticides （abamectin, azadirachtin, matrine, pyrethrins and imidacloprid）, and Neoseiulus cucumeris （Oudemans）, the natural enemy of cotton red spider mite, were studied. N. cucumeris was released after application of pesticides for seven days （for 0,3% azadirachtin EC） or six days （for other pesticides）. The results showed that the combined action of 1.8% abamectin EW （1：8 000） and N. cucumeris had the best control efficacy of 96.63% at 20 days after N. cucumeris releasing. The control efficacies of N. cucumeds and 0.3% azadirachtin EC （1：250） were 59.7% and 90.1% after one day and 20 days, respectively, after N. cucumeris releasing. The control efficacy of N. cucumeris and 0.5% matrine AS （1：2 000） was 82.65% at 20 days after N. cucumeris releasing. The results provide options for sustainable control of T. cinnabarinus and for the delay of pesticides resistance.
