Effect of Curcumin on Influenza Virus HIN1 and H3N2 in Vitro

Objective：To investigate the inhibitory effect of curcumin on influenza virus HIN1 and H3N2 in vitro, Methods：The directly killing role of cureumin extract in vitro to influenza virus type A subtype H1N1 and H3N2 was evaluated by the canine kidney cells （MDCK）, Results：The largest non toxic concentration of curcumin extract was 12, 5g/L and the effective inhibitory concentration to H1N1 and H3N2 was 6, 25G/1 AND 1,56g/L respectively, Conclusion： Curcumin extract have directly killing effect on H1N1 and H3N2 infections.

Chemical Compositions of Volatile Oil from Fruiting Body of Armillaria luteo-virens

The chemical compositions of volatile oil from fruiting body of Armillaria luteo-virens in Qinghai Province were firstly analyzed with GC-MS and its relevant compositions were detected by calculating chromatographic peak area with normalized method. 21 peaks were separated and 13 compositions were identified which were mainly unsaturated fatty acids, taking 97.1% of the total volatile oil.

如何做好“控制总量、调整结构”的文章——部分地区人行行长、总经济师接受本刊记者吴春明采访时的谈话

今年的货币信贷总方针是“控制总量、调整结构,保证重点、压缩一般,适时调节”,而调整结构是这个总方针中的一条红线,怎样从“调整结构”上作好文章就成了做好今年货币信贷工作的关键。为此,本刊记者吴春明就这一专题分别采访了人民银行漳州、三明、龙岩、福州的行领导。下面是他们对记者的谈话。 人民银位漳州市分行行长黄实俭:

强化银行信贷资产质量管理刻不容缓

强化银行信贷资产质量管理刻不容缓黄实俭信贷资金的流动性、安全性、效益性是银行工作的出发点和归宿点。然而，随着社会主义市场经济体制的建立，工商企业的生产、经营形式发生了很大的变化，这给银行经营带来了新的风险。对此，本文就漳州市专业银行逾期呆滞呆帐贷款情...

国务院安委会办公室关于江西宜春“6·16”重大道路交通事故的通报

安委办明电[2009]40号各省、自治区、直辖市及新疆生产建设兵团安全生产委员会: 2009年6月16日,一辆号牌为赣J32773面包车(核载7人,实载11人),由江西省宜春市开往萍乡市。

Effects of emodin and double blood supplies on liver regeneration of reduced size graft liver in rat model

AIM: To study the influences of emodin and reconstruction of double blood supplies on liver regeneration of reduced size graft liver in rat model.METHODS: A total of 45 SD-SD rat reduced size liver transplantation models were randomly divided into three groups (A-C). The conventional reduced size liver transplantation was performed on rats in group A, while the hepatic artery blood supply was restored in groups B and C. The emodin (1.5 mg/kg/d) was given by intraperitoneal route in group C only. The recipients were killed on the seventh day after the operation. The proliferative cell nuclear antigen (PCNA), TBil and ALT of serum were detected, and the pathological changes of liver cell were observed. RESULTS: The numbers of the rats that survived in A, B, and C group on the seventh day after operation were 14, 13, 13, respectively. The levels of TBil (31.5±5.2 μmol/L,23.2±3.1 μmol/L vs38.6±6.8 μmol/L), and ALT (5 351±1 050 nKat, 1300±900 nKat vs5779±1202 nKat) in serum in groups B and C were lower than those in group A(P＜0.05), while the expression of PCNA in groups B or C was higher than that in group A (22.0±3.5%, 28.2±4.2%vs18.6±3.2%, P＜0.05). The deeper staining nuclei, double nuclei, multi-nuclei and much glycogen were observed in liver cells of groups B and C, especially in group C, while fewer were found in liver cells of group A. CONCLUSION: The reconstruction of arterial blood supply is very important for rat liver regeneration after reduced size liver transplantation. Emodin has the effect of promoting liver regeneration and improving liver function in rats after reduced size transplantation. The possible mechanism is improving proliferation of liver cell and protecting liver cells from injury.

Effects of freeze-thaw cycle on engineering properties of loess used as road fills in seasonally frozen ground regions,North China

Compacted loess is widely used as fills of road embankments in loess regions of northern China.Generally, densely-compacted loess can satisfy the requirements of embankment strength and postconstruction deformation. However, uneven subsidence, pavement cracks and other related damages can affect the integrity of loess subgrade after several years of operation,and even cause some hazards, especially in North China, where the strong freeze-thaw erosion occurs. In this study, cyclic freeze-thaw tests for both densely and loosely compacted loess samples were performed to determine the variation in engineering properties such as volume, void ratio, collapsible settlement,microstructure, and the related mechanisms were addressed. The experimental results showed that an obvious water migration and redistribution occurred within the samples during freeze-thaw cycles. Ice lenses and fissures could be identified in the upper frozen layers of the samples. After freeze-thaw cycles,the dry densities of the upper layers of samples changed significantly due to strong freeze-thaw erosion. The dry densities decreased for the dense sample and increased for the loose sample. It can be found that dense samples become loose, while loose samples became dense with the increasing number of freeze-thaw cycles. Their related void ratios changed reversely. Both void ratios tended to fall into a certain range, which verified the concept of a residual void ratio proposed by Viklander. The loosening process of densely compacted samples involves the formation of large pores, volume increase and density reduction as well as the related changes in mechanical properties because freeze-thaw cycles may be important contribution to problems of loess road embankments.Adverse effects of freeze-thaw cycles, therefore,should be taken into account in selecting loess parameters for the stability evaluation of road embankment in seasonally frozen ground regions.

Effects of emodin on treating murine nonalcoholic fatty liver induced by high caloric laboratory chaw

AIM: To investigate the effects of emodin on the treatment of non-alcoholic fatty liver in rats induced by high caloric laboratory chaw. METHODS: Non-alcoholic fatty liver model was successfully established by feeding with high caloric laboratory chaw for 12 wk. Then the model rats were randomly divided into 3 groups, namely model control group, emodin group and dietary treatment group. The rats in emodin group were given emodin at dose of 40 mg/(kg·d) while animals in other groups were given distilled water of the same volume. The rats in model control group were fed with high caloric laboratory chaw while animals in other groups were fed with normal diet. Four weeks later, liver index (liver/body weight ratio), serum activities of liver-associated enzymes, blood lipid, fasting blood glucose, fasting plasma insulin, HOMA insulin resistance index (HOMA-IR), hepatic triglyceride content and histology features of all groups were assayed. The expression of hepatic peroxisomal proliferator activated receptor (PPAR) gamma was determined by RT-PCR. RESULTS: The body weight, liver index, serum activities of alanine aminotransferase (ALT), blood lipid, hepatic triglyceride content of model control group were significantly elevated, with moderate to severe hepatocyte steatosis. The expression of hepatic PPAR gamma mRNA was obviously reduced in model control group. Compared with model control group, the body weight, liver index, serum activities of ALT, blood lipids and hepatic triglyceride of emodin group significantly decreased and hepatic histology display was also greatly improved. Meanwhile, the expression of hepatic PPAR gamma mRNA was elevated. However, high serum activities of ALT and hyperlipidemia were persisted in dietary treatment group although liver index was decreased and liver histology was somewhat improved. CONCLUSION: It is suggested that emodin might be effective in the treatment of non-alcoholic fatty liver in rats. Its therapeutic mechanism could be associated with increasing the expression of hepatic PPAR gamma mRNA.

Emodin promoted pancreatic claudin-5 and occludin expression in experimental acute pancreatitis rats

AIM:To investigate the effect of emodin on pancreatic claudin-5 and occludin expression,and pancreatic paracellular permeability in acute pancreatitis(AP).METHODS:Experimental pancreatitis was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct.Emodin was injected via the external jugular vein 0 or 6 h after induction of AP.Rats from sham operation and AP groups were injected with normal saline at the same time.Samples of pancreas were obtained 6 or 12 h after drug administration.Pancreatic morphology was examined with hematoxylin and eosin staining.Pancreatic edema was estimated by measuring tissue water content.Tumor necrosis factor(TNF)-α and interleukin(IL)-6 level were measured by enzyme-linked immunosorbent assay.Pancreatic paracellular permeability was assessed by tissue dye extravasation.Expression of pancreatic claudin-5 and occludin was examined by immunohistology,quantitative real-time reverse transcriptase polymerase chain reaction and western blotting.RESULTS:Pancreatic TNF-α and IL-6 levels,wet/dry ratio,dye extravasation,and histological score were significantly elevated at 3,6 and 12 h following sodium taurocholate infusion;treatment with emodin prevented these changes at all time points.Immunostaining of claudin-5 and occludin was detected in rat pancreas,which was distributed in pancreatic acinar cells,ductal cells and vascular endothelial cells,respectively.Sodium taurocholate infusion significantly decreased pancreatic claudin-5 and occludin mRNA and protein levels at 3,6 and 12 h,and that could be promoted by intravenous administration of emodin at all time points.CONCLUSION:These results demonstrate that emodin could promote pancreatic claudin-5 and occludin expression,and reduce pancreatic paracellular permeability.

Virtual Huanghe River System:Framework and Technology

Virtual Reality provides a new approach for geographical research. In this paper, a framework of the Virtual Huanghe （Yellow） River System was first presented from the view of technology, which included five main modules——data sources, 3D simulation terrain database, 3D simulation model database, 3D simulation implementation and application system. Then the key technoiogies of constructing Virtual Huanghe River System were discussed in detail： 1） OpenGL technology, the 3D graphics developing instrument, was employed in Virtual Huanghe River System to realize the function of dynamic real-time navigation. 2） MO and OpenGL technologies were used to make the mutual response between 3D scene and 2D electronic map available, which made use of the advantages of both 3D scene and 2D electronic map, with the macroscopic view, integrality and conciseness of 2D electronic map combined with the locality, reality and visualization of 3D scene. At the same time the disadvantages of abstract and ambiguity of 2D electronic map and the direction losing of virtual navigation in 3D scene were overcome.

Treatment of flotation wastewater using biological activated carbon

A laboratory scale up-flow biological activated carbon(BAC) reactor was constructed for the advanced treatment of synthetic flotation wastewater. Biodegradation of a common collector(i.e., ethyl xanthate) for non-ferrous metallic ore flotation was evaluated. The results show that the two stages of domestication can improve microbial degradation ability. The BAC reactor obtains a chemical oxygen demand(COD) reduction rate of 82.5% for ethyl xanthate and its effluent COD concentration lowers to below 20 mg/L. The kinetics equation of the BAC reactor proves that the activated carbon layers at the height of 0 mm to 70 mm play a key role in the removal of flotation reagents. Ultraviolet spectral analysis indicates that most of the ethyl xanthate are degraded by microorganisms after advanced treatment by the BAC reactor.

Experimental study of therapeutic efficacy of Baicalin in rats with severe acute pancreatitis

AIM： To observe the therapeutic efficacy of Baicalin in rats with severe acute pancreatitis （SAP） and explore its therapeutic mechanisms. METHODS： The SAP rat models were randomly divided into the model control group, Baicalin treatment group, octreotide treatment group and sham operation group. All groups were randomly subdivided into 3 h, 6 h and 12 h groups with 15 rats in each group. The survival, ascites volume and pathological changes of pancreas in all rats were observed at different time points after operation. The plasma amylase content and serum TNF-α, IL-6, malonaldehyde （MDA） and PLA2 contents were also determined. RESULTS： The survival was not obviously different between the treated groups, and was significantly higher in treated groups at 12 h compared to the model control group （P 〈 0.05, 15 vs 10）. The ascites/body weight ratio at 3 h and 6 h was significantly lower in Baicalin treatment group compared to the model control group and octreotide treatment group （P 〈 0.05, 1.00 vs 2.02 and 1.43 and P 〈 0.001, 2.29 （1.21） vs 2.70 （0.80） and 2.08 （2.21）, respectively）. The contents of amylase, TNF-α, IL-6, MDA and PLA2 were significantly lower in the treated groups than in the model control group （P 〈 0.05, 4342 vs 5303, 5058 vs 6272 in amylase, P 〈 0.01, 21.90 vs 36.30, 23.80 vs 39.70, 36 vs 54.35 in MDA and 56.25 vs 76.10 in PIA2, or P 〈 0.001, 65.10 and 47.60 vs 92.15 in TNF-α, 3.03 vs 5.44, 2.88 vs 6.82, 2.83 vs 5.36 in IL-6, respectively）. The pathological scores of pancreas in the treated groups were significantly lower than that in the model control group （P 〈 0.05, 9.00 vs 10.05, 6.00 vs 9.00, 8.00 vs 10.05）, but no marked difference was found between the treated groups. CONCLUSION： The Baicalin injection has significant therapeutic effects on SAP rats, its effects are similar to those of octreotide. The Baicalin injection is also cheap and has a big application range, quite hopefully to be used in clinical treatment of SAP.

Wheat Grain Yield and Yield Stability in a Long-Term Fertilization Experiment on the Loess Plateau

To provide a scientific basis for sustainable land management, a 20-year fertility experiment was conducted in Changwu County, Shaanxi Province, China to investigate the effects of long-term application of chemical fertilizers on wheat grain yield and yield stability on the Loess Plateau using regression and stability analysis. The experiment consisted of 17 fertilizer treatments, containing the combinations of different N and P levels, with three replications arranged in a randomized complete block design. Nitrogen fertilizer was applied as urea, and P was applied as calcium superphosphate. Fertilizer rates had a large effect on the response of wheat yield to fertilization. Phosphorus, combined with N, increased yield significantly （P 〈 0.01）. In the unfertilized control and the N or P sole application treatments, wheat yield had a declining trend although it was not statistically significant. Stability analysis combined with the trend analysis indicated that integrated use of fertilizer N and P was better than their sole application in increasing and sustaining the productivity of rainfed winter wheat.

Protective Effect of Immaturue Bitter Orange(Citrus aurantium L.)Flavonoids Extracts on PC12 Cell Injury Induced by 6-Hydroxydopamine

To study the neuro protective effect of flavonoids extracts from immature bitter orange(Citrus aurantium L.),the PC12 cells treated with 6-hydroxydopamine(6-OHDA)were used as the Parkinson’s disease(PD)model.To determine the optimal dose of 6-OHDA for constructing a PD model,PC12 cells were incubated with different concentrations of 6-OHDA for 24 h.After 24 h incubation,PC12 cells of drug groups were added 6-OHDA and different concentrations of flavonoids extracts were measured cell viability by CCK8 for selecting effective concentration of flavonoids extracts;the ROS level was determined using flow cytometry;the levels of MDA,CAT,SOD and GSH-Px were assayed by Colorimetric kit for oxidative stress investigation.Compared with the model group,PC12 cell viability was significantly enhanced(P<0.05),the levels of ROS and MDA were reduced significantly(P<0.05),and the activities of SOD,CAT and GSH-Px were significantly enhanced(P<0.05)in drug groups.In conclusion,immature bitter orange flavonoids extracts could protect PC12 cells against 6-OHDA-induced oxidative stress.

Nano-microbubble flotation of fine and ultrafine chalcopyrite particles

As is well known to mineral processing scientists and engineers, fine and ultrafine particles are difficult to float mainly due to the low bubble-particle collision efficiencies. Though many efforts have been made to improve flotation performance of fine and ultrafine particles, there is still much more to be done. In this paper, the effects of nano-microbubbles （nanobuhbles and microbubbles） on the flotation of fine （-38 ＋ 14.36 μm） and ultrafine （-14.36 ＋ 5μm） chalcopyrite particles were investigated in a laboratory scale Denver flotation cell. Nano-microbubbles were generated using a specially-designed nano- microbubble generator based on the cavitation phenomenon in Venturi tubes. In order to better under- stand the mechanisms of nano-microbubble enhanced froth flotation of fine and ultrafine chalcopyrite particles, the nano-microbubble size distribution, stability and the effect of frother concentration on nano- bubble size were also studied by a laser diffraction method. Comparative flotation tests were performed in the presence and absence of nano-microbubbles to evaluate their impact on the fine and ultrafine chalcopyrite particle flotation recovery. According to the results, the mean size of nano-microbubbles increased over time, and decreased with increase of frother concentration. The laboratory-scale flotation test results indicated that flotation recovery of chalcopyrite fine and ultrafine particles increased by approximately 16-21% in the presence of nano-microbubbles, depending on operating conditions of the process. The presence of nano-microbubbles increased the recovery of ultrafine particles （-14.36 ＋ 5 μm） more than that of fine particles （-38 ＋ 14.36 μm）. Another major advantage is that the use of nano-microbubbles reduced the collector and frother consumptions by up to 75% and 50%, respectively.

The Bitter Butter Knife＂ Puttermesser＇s Problematic Paradise

This paper analyzes how the themes of magical realism and female divinity intersect in the novel The Puttermesser Papers （1997） by Cynthia Ozick. In the ＂Introduction＂, the writer defines magical realism and discusses its connections to Women＇s Studies. The next section, ＂The Bitter Butter Knife＂, discusses the protagonist＇s （Ruth Puttermesser） boring existence and pathetic attempts to connect to her Jewish ancestry. In ＂The Problematic Paradise＂, the author focuses on Puttermesser＇s attempts to take control of her life by creating the first female golem and the ups and downs of paradise. This author argues that the female protagonist of the novel utilizes magical realism as a tool of empowerment over personal oppression.

Effects of Lactobacillus plantarum on gut barrier function in experimental obstructive jaundice

AIM:To investigate the mechanisms of Lactobacillus plantarum(L.plantarum)action on gut barrier in preoperative and postoperative experimental obstructive jaundice in rats.METHODS:Forty rats were randomly divided into groups of sham-operation,bile duct ligation(BDL),BDL +L.plantarum,BDL+internal biliary drainage(IBD),and BDL+IBD+L.plantarum.Ten days after L.plantarum administration,blood and ileal samples were collected from the rats for morphological examination,and intestinal barrier function,liver function,intestinal oxidative stress and protein kinase C(PKC)activity measurement.The distribution and expression of the PKC and tight junction(TJ)proteins,such as occludin,zonula occludens-1,claudin-1,claudin-4,junction adhesion molecule-A and F-actin,were examined by confocal laser scanning microscopy,immunohistochemistry,Western blotting,real-time fluorescent quantitative polymerase chain reaction assay.RESULTS:L.plantarum administration substantially restored gut barrier,decreased enterocyte apoptosis,improved intestinal oxidative stress,promoted the activity and expression of protein kinase(BDL vs BDL+L.plantarum,0.295±0.007 vs 0.349±0.003,P<0.05;BDL+IBD vs BDL+IBD+L.plantarum,0.407±0.046 vs 0.465±0.135,P<0.05),and particularly enhanced the expression and phosphorylation of TJ proteins in the experimental obstructive jaundice(BDL vs BDL+L.plantarum,0.266±0.118 vs 0.326±0.009,P<0.05).The protective effect of L.plantarum was more prominent after internal biliary drainage(BDL+IBD vs BDL +IBD+L.plantarum,0.415±0.105 vs 0.494±0.145,P<0.05).CONCLUSION:L.plantarum can decrease intestinal epithelial cell apoptosis,reduce oxidative stress,and prevent TJ disruption in biliary obstruction by activating the PKC pathway.

Effect of Astragalus complanatus flavonoid on anti-liver fibrosis in rats

AIM： To observe the anti-liver fibrosis effect of Astragalus complanatus fiavonoids （ACF） in rats. METHODS： The liver fibrosis model in rats was established by injecting interperitoneally 0.2 mL/100 g 0.5% dimethylnitrosamine, thrice a week. Meanwhile, the rats were administered ACF （30, 60, 120 mg/kg） or colchicine （0.1 mg/kg） once a day for 1 mo. Serum N-propeptide of type Ⅰ procollagen （PINP） and type Ⅲ procollagen （PⅢNP） was measured using ELISA. Malondialdehyde （MDA） and superoxide dismutase （SOD） in hepatic tissue were evaluated. Matrix metal protease-1 （MMP-1） mRNA expression was assayed by RT-PCR and the protein expression of tissue inhibitor of metal protease-1 （TIMP-1） was analyzed by immunohistochemistry. RESULTS： In the ACF groups, SOD activity increased and MDA content decreased in comparison to the liver fibrosis model group. The serum PINP and PⅢNP contents in ACF-2 and -3 group decreased compared to those in model group. In ACF-2 and -3 group, the expression of MMP-1 mRNA increased significantly and the protein expression of TIMP-1 decreased compared to that in model group. CONCLUSION： The antifibrotic mechanisms of ACF are associated with its influence on lipid peroxidation and collagen synthesis and degradation.

Estrogen and progesterone receptor isoforms expression in the stomach of Mongolian gerbils

AIM: We studied the estrogen receptor (ER) and progesterone receptor (PR) isoforms expression in gastric antrum and corpus of female gerbils and their regulation by estradiol (E2) and progesterone (P4). METHODS: Ovariectomized adult female gerbils were subcutaneously treated with E2, and E2 + P4. Uteri and stomachs were removed, the latter were cut along the greater curvature, and antrum and corpus were excised. Proteins were immunoblotted using antibodies that recognize ER-alpha, ER-beta, and PR-A and PR-B receptor isoforms. Tissues from rats treated in the same way were used as controls. RESULTS: Specific bands were detected for ER-alpha (68 KDa), and PR isoforms (85 and 120 KDa for PR-A and PR-B isoforms, respectively) in uteri, gastric antrum and corpus. We could not detect ER-beta isoform. PR isoforms were not regulated by E2 or P4 in uterus and gastric tissues of gerbils. ER-alpha isoform content was significantly down-regulated by E2 in the corpus, but not affected by hormones in uterus and gastric antrum. CONCLUSION: The presence of ER-alpha and PR isoforms in gerbils stomach suggests that E2 and P4 actions in this organ are in part mediated by their nuclear receptors.

Design and Experimental Analysis of NLT Trussed Rafter in Yellow Pine

The roof system of social housing in Brazil generally consists of components made out of native forest lumber of high market value. Taking into account the increasing number of planted forests and the need to develop new products and to add value to this timber, this work deals with the development and structural analysis of a roof system using yellow pine plantation wood （Pinus spp）, a sustainable material which however presents many defects. The NLT （laminated nailed timber） technology was chosen as it allows the use of shorter length and smaller cross section pieces, eliminating major defects. Seven samples of structural trussed rafters in NLT were tested; six made out of graded timber and one ungraded in order to verify the impact of the wood grading in the structural performance of the model. The results showed that the trussed rafter system in NLT meets the necessary structural performance requiring poor conditions of infrastructure for manufacture process, and that the graded wood samples showed better performance than the ungraded one.