目的:观察芪黄疽愈方对下肢动脉硬化闭塞症(arteriosclerosis occlusive disease of the lower extremities,ASOLE)大鼠肝脏脂质代谢相关因子B类Ⅰ型清道夫受体(scavenger receptor class B typeⅠ,SR-BⅠ)、过氧化物酶体增殖物激活受体...目的:观察芪黄疽愈方对下肢动脉硬化闭塞症(arteriosclerosis occlusive disease of the lower extremities,ASOLE)大鼠肝脏脂质代谢相关因子B类Ⅰ型清道夫受体(scavenger receptor class B typeⅠ,SR-BⅠ)、过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptor,PPAR)-α及单核细胞趋化蛋白1(monocyte chemotactic protein-1,MCP-1)表达的影响。方法:高脂饮食联合隐动脉内膜损伤法制作大鼠ASOLE模型,Western印迹检测肝脏SR-BⅠ,ELISA检测肝脏PPAR-α、MCP-1。结果:与空白组比较,模型组SR-BⅠ表达明显降低,芪黄疽愈方处理后表达明显升高;模型组PPAR-α较空白组显著性降低,芪黄疽愈方处理升高PPAR-α水平,其中高、中浓度组与模型组有显著性差异;与空白组比较,模型组MCP-1显著性升高,芪黄疽愈方处理降低MCP-1水平,其中高浓度组较模型组显著性下降。结论:芪黄疽愈方能够升高SR-BⅠ、PPAR-α,降低MCP-1水平,可能与其调节脂质代谢从而抑制炎症反应具有相关性。展开更多
Background: The diagnosis of autoimmune pancreatitis can be difficult and ofte n requires a larger specimen than can be provided by FNA alone to determine if t he tissue sample obtained with EUS trucut biopsy (TCB) is...Background: The diagnosis of autoimmune pancreatitis can be difficult and ofte n requires a larger specimen than can be provided by FNA alone to determine if t he tissue sample obtained with EUS trucut biopsy (TCB) is sufficient to allow ad equate histologic review to establish the diagnosis of autoimmune pancreatitis. Methods: EUS TCB was performed in patients presenting with obstructive jaundice who were suspected of having autoimmune pancreatitis based on their clinical, la boratory and imaging studies. The charts were retrospectively reviewed to determ ine the feasibility of TCB. Results: Between August 2002 and June 2004, 3 patien ts with obstructive jaundice and suspected autoimmune pancreatitis (AIP) underwe nt EUS TCB. In each case, a diagnosis of pancreatic cancer also was considered, and surgical resection was the planned therapy before the patient underwent EUS TCB. Histologic review of the TCB specimens established the diagnosis of AIP in two patients and identified nonspecific changes of chronic pancreatitis in the t hird patient. EUS-guided FNA was performed in two of the 3 patients and failed to establish the diagnosis in either patient. Other than mild transient abdomina l pain (n = 1), no complications were identified. Conclusions: This preliminary study suggests that EUS TCB can safely establish the diagnosis of AIP. Doing so helps guide management and may help to avoid unnecessary surgery. Prosp- ective studies are needed to verify these findings and to more clearly define the role of EUS TCB in these patients.展开更多
文摘目的:观察芪黄疽愈方对下肢动脉硬化闭塞症(arteriosclerosis occlusive disease of the lower extremities,ASOLE)大鼠肝脏脂质代谢相关因子B类Ⅰ型清道夫受体(scavenger receptor class B typeⅠ,SR-BⅠ)、过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptor,PPAR)-α及单核细胞趋化蛋白1(monocyte chemotactic protein-1,MCP-1)表达的影响。方法:高脂饮食联合隐动脉内膜损伤法制作大鼠ASOLE模型,Western印迹检测肝脏SR-BⅠ,ELISA检测肝脏PPAR-α、MCP-1。结果:与空白组比较,模型组SR-BⅠ表达明显降低,芪黄疽愈方处理后表达明显升高;模型组PPAR-α较空白组显著性降低,芪黄疽愈方处理升高PPAR-α水平,其中高、中浓度组与模型组有显著性差异;与空白组比较,模型组MCP-1显著性升高,芪黄疽愈方处理降低MCP-1水平,其中高浓度组较模型组显著性下降。结论:芪黄疽愈方能够升高SR-BⅠ、PPAR-α,降低MCP-1水平,可能与其调节脂质代谢从而抑制炎症反应具有相关性。
文摘Background: The diagnosis of autoimmune pancreatitis can be difficult and ofte n requires a larger specimen than can be provided by FNA alone to determine if t he tissue sample obtained with EUS trucut biopsy (TCB) is sufficient to allow ad equate histologic review to establish the diagnosis of autoimmune pancreatitis. Methods: EUS TCB was performed in patients presenting with obstructive jaundice who were suspected of having autoimmune pancreatitis based on their clinical, la boratory and imaging studies. The charts were retrospectively reviewed to determ ine the feasibility of TCB. Results: Between August 2002 and June 2004, 3 patien ts with obstructive jaundice and suspected autoimmune pancreatitis (AIP) underwe nt EUS TCB. In each case, a diagnosis of pancreatic cancer also was considered, and surgical resection was the planned therapy before the patient underwent EUS TCB. Histologic review of the TCB specimens established the diagnosis of AIP in two patients and identified nonspecific changes of chronic pancreatitis in the t hird patient. EUS-guided FNA was performed in two of the 3 patients and failed to establish the diagnosis in either patient. Other than mild transient abdomina l pain (n = 1), no complications were identified. Conclusions: This preliminary study suggests that EUS TCB can safely establish the diagnosis of AIP. Doing so helps guide management and may help to avoid unnecessary surgery. Prosp- ective studies are needed to verify these findings and to more clearly define the role of EUS TCB in these patients.
