Sporadic motor neuron disease (MND) causes a progressive loss of motor neuron s. West Nile virus can attack motor neurons, so we examined whether flavivirus i nfection could be detected in MND cases. Spinal cord secti...Sporadic motor neuron disease (MND) causes a progressive loss of motor neuron s. West Nile virus can attack motor neurons, so we examined whether flavivirus i nfection could be detected in MND cases. Spinal cord sections from 22 MND cases were stained immunohistochemically with a flavivirus- specific antibody. No sta ining for flavivirus was seen in any case. Sporadic MND does not appear to arise from a recent infection with a flavivirus.展开更多
The development of hepatocellular carcinoma （HCC） is attributed to several factors, including chronic viral infection, alcohol consumption, exposure to aflatoxin 131 and metabolic disorders. Several recent reports h...The development of hepatocellular carcinoma （HCC） is attributed to several factors, including chronic viral infection, alcohol consumption, exposure to aflatoxin 131 and metabolic disorders. Several recent reports have shown that HCC can occur in patients with longstanding Crohn＇s disease （CD） in the absence of other underlying high-risk liver diseases. There may be an association between CD and hepatocarcinogenesis, however, the precise mechanism for this requires further investigations.展开更多
To elucidate the molecular response of shrimp hepatopancreas to white spot syndrome virus(WSSV) infection, microarray was applied to investigate the differentially expressed genes in the hepatopancreas of ‘Huanghai N...To elucidate the molecular response of shrimp hepatopancreas to white spot syndrome virus(WSSV) infection, microarray was applied to investigate the differentially expressed genes in the hepatopancreas of ‘Huanghai No. 2'(Fenneropenaeus chinensis). A total of 59137 unigenes were designed onto a custom-made 60 K Agilent chip. After infection, the gene expression profiles in the hepatopancreas of the shrimp with a lower viral load at early(48–96 h), peak(168–192 h) and late(264–288 h) infection phases were analyzed. Of 18704 differentially expressed genes, 6412 were annotated. In total, 5453 differentially expressed genes(1916 annotated) expressed at all three phases, and most of the annotated were either up-or down-regulated continuously. These genes function diversely in, for example, immune response, cytoskeletal system, signal transduction, stress resistance, protein synthesis and processing, metabolism among others. Some of the immune-related genes, including antilipopolysaccharide factor, Kazal-type proteinase inhibitor, C-type lectin and serine protease encoding genes, were up-regulated after WSSV infection. These genes have been reported to be involved in the anti-WSSV responses. The expression of genes related to the cytoskeletal system, including β-actin and myosin but without tubulin genes, were down-regulated after WSSV infection. Astakine was found for the first time in the WSSV-infected F. chinensis. To further confirm the expression of differentially expressed genes, quantitative real-time PCR was performed to test the expression of eight randomly selected genes and verified the reliability and accuracy of the microarray expression analysis. The data will provide valuable information to understanding the immune mechanism of shrimp's response to WSSV.展开更多
An emerging mosquito-borne arbovirus named Zika virus(ZIKV), of the family Flaviviridae and genus Flavivirus, is becoming a global health threat. ZIKV infection was long neglected due to its sporadic nature and mild s...An emerging mosquito-borne arbovirus named Zika virus(ZIKV), of the family Flaviviridae and genus Flavivirus, is becoming a global health threat. ZIKV infection was long neglected due to its sporadic nature and mild symptoms. However, recently, with its rapid spread from Asia to the Americas, affecting more than 30 countries, accumulating evidences have demonstrated a close association between infant microcephaly and Zika infection in pregnant women. Here, we reviewed the virological, epidemiological, and clinical essentials of ZIKV infection.展开更多
文摘Sporadic motor neuron disease (MND) causes a progressive loss of motor neuron s. West Nile virus can attack motor neurons, so we examined whether flavivirus i nfection could be detected in MND cases. Spinal cord sections from 22 MND cases were stained immunohistochemically with a flavivirus- specific antibody. No sta ining for flavivirus was seen in any case. Sporadic MND does not appear to arise from a recent infection with a flavivirus.
文摘The development of hepatocellular carcinoma （HCC） is attributed to several factors, including chronic viral infection, alcohol consumption, exposure to aflatoxin 131 and metabolic disorders. Several recent reports have shown that HCC can occur in patients with longstanding Crohn＇s disease （CD） in the absence of other underlying high-risk liver diseases. There may be an association between CD and hepatocarcinogenesis, however, the precise mechanism for this requires further investigations.
基金the Central Public-interest Scientific Institution Basal Research Fund, CAFS (No. 2016HY-ZD04)the National Natural Science Foundation of China (No. 31372523)+2 种基金the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02-03)the Taishan Scholar Program For Seed Industry, the Shandong Provincial Natural Science Foundation (No. ZR2014CQ001)the Special Fund for Postdoctoral Innovative Project of Shandong Province, and Central Public-Interest Scientific Institution Basal Research Fund of the Yellow Sea Fisheries Research Institute (Nos. 2060302013036 and 20603022015013)
文摘To elucidate the molecular response of shrimp hepatopancreas to white spot syndrome virus(WSSV) infection, microarray was applied to investigate the differentially expressed genes in the hepatopancreas of ‘Huanghai No. 2'(Fenneropenaeus chinensis). A total of 59137 unigenes were designed onto a custom-made 60 K Agilent chip. After infection, the gene expression profiles in the hepatopancreas of the shrimp with a lower viral load at early(48–96 h), peak(168–192 h) and late(264–288 h) infection phases were analyzed. Of 18704 differentially expressed genes, 6412 were annotated. In total, 5453 differentially expressed genes(1916 annotated) expressed at all three phases, and most of the annotated were either up-or down-regulated continuously. These genes function diversely in, for example, immune response, cytoskeletal system, signal transduction, stress resistance, protein synthesis and processing, metabolism among others. Some of the immune-related genes, including antilipopolysaccharide factor, Kazal-type proteinase inhibitor, C-type lectin and serine protease encoding genes, were up-regulated after WSSV infection. These genes have been reported to be involved in the anti-WSSV responses. The expression of genes related to the cytoskeletal system, including β-actin and myosin but without tubulin genes, were down-regulated after WSSV infection. Astakine was found for the first time in the WSSV-infected F. chinensis. To further confirm the expression of differentially expressed genes, quantitative real-time PCR was performed to test the expression of eight randomly selected genes and verified the reliability and accuracy of the microarray expression analysis. The data will provide valuable information to understanding the immune mechanism of shrimp's response to WSSV.
基金supported by the National Natural Science Foundations of China(81471957,81271839,81372935,81301435,and 81401676)
文摘An emerging mosquito-borne arbovirus named Zika virus(ZIKV), of the family Flaviviridae and genus Flavivirus, is becoming a global health threat. ZIKV infection was long neglected due to its sporadic nature and mild symptoms. However, recently, with its rapid spread from Asia to the Americas, affecting more than 30 countries, accumulating evidences have demonstrated a close association between infant microcephaly and Zika infection in pregnant women. Here, we reviewed the virological, epidemiological, and clinical essentials of ZIKV infection.
