潜心科研创新 预防地震新方向——记地震预防研究者黄纯忠的创新路

2008年＂5.12＂汶川特大地震,震撼了全世界,地震吞没了灾区人民,摧毁了房屋,迸垮了山崖,造成了大面积惨不忍睹的场面。也正是这场地震,再次激起了一个人向地震宣战的愿望。他就是善于总结、发现新问题、研究新事物的黄纯忠。鉴于目前国内外预测技术对地震的预测、预报效果欠佳的前堤,关键是未能捕获地震前准确的震源信息。黄纯忠先生进行了广泛的宏观研究,

Optimization of Preparative Separation and Purification of Total Flavonoids from Radix Puerariae by Macroporous Resin Method

Aim To screen the optimum macroporous resin and conditions for the isolation and purification of flavonoids from Radix Puerariae. Methods The static and dynamic adsorption/desorption methods were used, and the separation and purification process was evaluated by measuring the concentration of total flavonoid in the fractions with UV spectrophotometer. Results The SP70 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 0.5 g· mL^- 1 corresponding to crude drug for concentration of extract, pH 5 - 6, and appended 60 times the volume of the resin bed （BV） with the adsorption speed 2 BV·h^-1, and the volume of aq. 70% （V/V） ethanol as eluant was 5 BV with desorption speed 2 BV·h^-1. By this method, the final contents of total flavonoids exceeded 80%. Conclusion The SP70 macroporous resin is the most effective one for large-scale isolation and purification of flavonoids from Radix Pueraria, which meets industrial needs.

守住一杯茶

有时独自一人,就想选择在一个温暖的月夜里喝一杯茶,茶具并不讲究,是那种透明的玻璃高杯,茶叶也极普通,是那种常见的绿茶,只待一壶水冲进茶杯后,那绿茶就张开几十片嫩绿的叶片,在茶杯里上下冲撞回旋,最终潜伏在茶杯的杯口不再晃动。稍息片刻揭开茶盖轻吹一口气,那叶片并沿杯沿荡去。现出一片嫩黄的汤色来,那汤色金黄纯厚,在杯里摇摇晃晃,直到有一片月光跌落进茶杯,那颜色才深沉而动人起来,轻吹一口,那茶味与香。

我的幸福生活

我的生活里天天都有好吃的味道、感动的味道和开心的味道。 妈妈是个厨师,擅长烹饪各种美食。有一次,我在爬楼梯的时候就闻到了妈妈做晚饭时飘出来的菜香味。“啊,一定是我最爱吃的红烧肉!”我的口水都快流下来了。我飞奔回家,妈妈笑眯眯地说：“刚出锅的红烧肉,要不要吃一块？”我开心极了,大声喊道：“要!”说完,我迫不及待地夹起一块红烧肉大口大口地吃了起来。真香啊!这就是好吃的味道。

《工厂供电》复习提要

《工厂供电》是我区89级工业自动化专业和电力系统自动化两个专业第六学期开设的一门选修课,计划授课54学时,7.5学分。教材规定使用由天津大学出版社出版的黄纯华、刘维仲合编的《工厂供电》(中央广播电视大学教学用书)。由于中央电大没有统一播出该课,各教学班可请老师面授。但必须参加自治区电大统一命题考试.

市人事局专业干部管理座谈会强调必须坚持专业干部继续教育的制度

今年3月16日市人事局专业干部处和我院商经系联合召开了广州市商业口各局、总公司和外经贸系统各单位人事部门负责专业干部管理工作同志的座谈会。会议由人事局专业干部处负责同志主持。与会者就目前如何贯彻国家人事部关于认真抓好专业技术人员继续教育工作的指示精神。

Purification and characterization of vitellin in mature ovary of marine crab Charybdis japonica

Vitellin of mature female marine crab Charybdis japonica was purified by high-performance liquid chromatography (HPLC, DEAE-cellulose-16 anion exchange column). The apparent molecular mass of the vitellin is 546 ku based on the data of native-PAGE. Under denatured condition (SDS-PAGE), it was found that vitellin was composed of four polypeptides each at 120, 100, 65, and 55 ku. One disulfide bond was detected in the binding of polypeptide subunits. The purified vitellin, contained 4.47% phosphor and 10.6% polysaccharides, and was identified as glyco-lipo-carotenoprotein, according to the PAGE staining data. The purified vitellin can be used as antigen to raise polyclonal antisera in further application.

Improving Linear Type Traits to Improve Production Sustainability and Longevity in Purebred Sahiwal Cattle

In order to determine various traits which need to be improved for improving the productive life span and longevity, purebred Sahiwal cows available at bull mother experimental farm and cattle breeding farm located at Veterinary College, Durg, Chhattisgarh, India were used. For present investigation, 17 linear type traits were measured, scaled and scored as per the guidelines of International Committee for Animal Recording （2001）. The average score point （ASP） under 1-9 point scale score system along with respective observed group for different linear type traits were stature （6.88; taller）, chest width （5.30; intermediate）, body depth （4.11; intermediate）, rump angle （4.27; intermediate）, rump width （5.09; intermediate）, rear leg set-side view （4.55; intermediate）, rear leg set-rear view （5.95; intermediate）, foot angle （5.66; intermediate）, udder depth （5.71; intermediate）, rear udder height （4.90; intermediate）, udder balance （5.27; intermediate）, udder cleft （3.75; intermediate）, fore udder attachment （5.55; intermediate）, teat length （3.54; intermediate）, fore teat placement （5.33; intermediate）, rear teat placement （6.37; intermediate） and teat thickness （2.76; narrow）. For most of the traits, ASP which lies near midpoint （i.e. near five） is known to be ideal for dairy type cattle. Moreover, some traits also showed the presence of undesirable ASP. Hence, the traits such as body depth, rump angle, foot angle, udder depth, rear udder height, udder cleft, fore udder attachment, teat length, and teat thickness needs to be improved for improving the production sustainability and herd life of Sahiwal cattle. Thus, present investigation gives explicit clue to incorporate these conformation traits in selection program of this valuable germplasm commonly found in Southern part of Asia （India, Pakistan, Srilanka, etc.）.

The Effects of Natural Capital Protection on Pastoralist's Livelihood and Management Implication in the Source Region of the Yellow River, China

The interaction among different livelihood capitals is a key to generate a deeper understanding of the livelihood sustainability.In this paper,we use net income per capita(economic capital),meat and milk production per capita(physical capital),and areas of fenced pasture,livestock shelter,grassland rodent control and planted grassland(physical capital) as proxy indicators of livelihood promotion,livelihood provision,and livelihood protection respectively.By developing a correlation model between pastoralists' livelihood protection and improvement,we found that(1) there is a statistically significant correlation between the pastoralists' livelihood protection and promotion;(2) based on the maximum effect of pastoralists' livelihood promotion and provision,there is a benchmark in the effect of the intervention intensity of livelihood capital(grassland resource protection) on livelihood improvement;(3) on basis of two indicators,i.e.net income per capita and meat production per capita,the reasonable scales of fenced pasture,livestock shelter and planted grassland are less than 843,860 and 46 thousand hectares(hm2) per year respectively.With the marginal effect of livelihood protection,moderately decreased areas of fenced pasture and planted grassland,and increased area of livestock shelter is a critical to ensure pastoralist's livelihood sustainability.

Purification of Antimicrobial Peptide from Antarctic Krill (Euphausia superba) and its Function Mechanism

The preliminary purification and antimicrobial mechanism of antimicrobial peptide from Antarctic Krill were studied in this paper. The results showed that the molecular weight range of antimicrobial polypeptide （CMCC-1） obtained by cation exchange chromatography was between 245-709D as detected by molecular sieve chromatography, and the minimum inhibition concentration （MIC） of CMCC-1 against Staphylococcus aureus was 5.0mgmL^-1. The antimicrobial mechanism of CMCC-1 was studied with S. aureus as indicator bacterium. Compared with control group, the results of the experimental group in which S. aureus was treated with CMCC-1 were as follows： l） CMCC-1 could inhibit cell division at logarithmic phase. 2） The protein and reducing sugar con- tent, and the conductivity of culture medium increased, and the activity of alkaline phosphatase and [3-galactosidase could be detected in the culture medium. 3） Observation under scanning electron microscope revealed that somatic morphology became irregular, and then somatic surface became coarse. The cell became much smaller, and most somatic ceils gathered. The boundary between cells became dim and finally fused as a whole. 4） Observation under transmission electron microscope showed that the surface of S. aureus became rough and the reproducing ability was restrained. The cell wall became thin and the cytoplasm shrunk. Substances inside cell leaked out, which caused cells death. 5） SDS-PAGE analysis showed that some bands disappeared, and the residual bands became vague. 6） The genomic DNA electrophoresis results showed that the genomic DNA bands ofS. aureus were not degraded but the brightness significantly reduced. Thus, it is supposed that CMCC-1 could destroy the cell wall and membrane of S. aureu, increase the cell membrane permeability and the leaking-out of intracellular substances, and thus cause the death ofS. aureu.

PURIFICATION OF FLAVONES OF GINKGO LEAVES BY ADSORPTION HZ-841 RESIN

The purification process of total flavones of ginkgo leaves by resin HZ-841 from ethanol extract was studied. First, the total flavone was extracted from the defatted powder of ginkgo L. biloba leaves. Effects of solvents and operation conditions were examined to get a relative high yield and purity in this step. The crude extract was further purified by resin HZ-841. Both adsorption and elution process were studied to obtain an optimized conditions, i.e., pH, flow rate, concentration. A yellow powder was obtained, of which 37.3% was flavones, obviously higher than the basic international standard of 24%.

Purification and Partial Characterization of an Antibacterial Protein Tz1

A strain (T3) of Bacillus has been screened from paddy field. It secretes large amount of antibacterialproteins which show a strong inhibiting activity against several pathogens of rice. This paper presentsa systematic study of the inhibition spectrum and characteristics of T3 proteins. Total proteins wereprecipitated with ammonium sulfate at 70% saturation from cell-free culture. One of the proteins(Tzl) was purified from the crude extracts with Sephadex G-100, DEAE52 and FPLC Superose 12columns. A single band was demonstrated in both 15% SDS-PAGE and IEF, with an apparent MWof 6,9 kd and a pI of 7.8. Its amino acid composition was analyzed and part of its sequence,determined.

Comparison of methods of purification and identification of Verticillium dahliae toxins

The complexes excreted by VerticiUium dahliae are phytotoxins, which are responsible for most of the symptoms associated with Verticillium wilt disease. Verticillium dahliae toxins （VD-toxins） can be purified by different methods. In the present study, we reported a simpler, more effective method to purify VD-toxins. The supematant of V. dahliae culture was frozen, lyophilized and dialyzed by 1 kDa Dialysis Membranes （MWCO）. We also partially identified the characteristics of the purified VD-toxins. The results showed that the components of VD-toxins include glycoprotein within 35.8-83.2 kDa. The phytotoxic activity of VD-toxins was remained after VD-toxins were pretreated by high temperature, Concanavalin-A, and proteinase E, respectively. These data suggest that VD-toxins are heat-stable, and the protein fraction and glycosyl are both important contributors to the phytotoxic activity. VD-toxins purified effectively from the culture filtrates of V. dahliae may help in further understanding the mechanisms of interactions between V. dahliae and plants.

Isolation and Preparative Purification for Ginkgolides A and B

In this paper a simple preparative method for isolation and purification of ginkgolides A and B was developed,As starting material,a commercially available standardized ginkgo extract (EGb761,containing 24% flavonoid and 6% terpene trilactones) was used,After a pretreatment step,optimized by the uniform design method ,the concentrated intermediate extract with high content of GA and gb(+90%) was separated into the individual terpenes by preparative liquid chromatography eluted with petroleum ether-ethylacetate,Analysis of products was carried out by means of HPLC-ELSD(evaporative light -scattering detector),The results show that ginkgolides A and B are obtained in higher yield and better purity.

Separation and Purification Process of Total Flavones from Litchi Pericarp by Macroporous Absorption Resin

[ Objective] This study aimed to optimize the conditions for purification of total flavones from litchi pericarp by macroporous absorption resin. E Method] The flavones adsorption rates and desorption rates of macroporous absorption resins （AB-8, HPD-600, D101 ） were compared, and the technological parameters of D101 during the purification process were investigated. E Result] D101 macroporous absorption resin was ap- propriate for the purification of total flavonoids from litchi pericarp. The optimal technological conditions were selected .. the pH of sample solution was 5.0; concentration of sample solution was 4 mg/ml, with a volume of 2.5BV; 80% ethanol was used as elution solution, with a volume of 2.0BV. [ Condusion] The content of total flavones achieved 83% after separation by D101 macoporous absorption resin.

近十年来的国内唐宋专卖史研究

近十年来的国内唐宋专卖史研究黄纯艳专卖制度史是中国经济史研究中的重要问题。唐宋两代是专卖制发展承前启后的转折时期。在制度上，实现了由直接专卖向以间接专卖为主的变革性过渡，出现了若干新的政策、形式，并为后世沿用；一些新的商品，如茶、香、矾、醋等被纳入专...

独生子女继承遗产成难题?

如果您觉得独生子女是父母的唯一继承人,继承全部遗产是顺理成章、自然而然的事,那您可就想简单了。如果父母突然离世,没有留下有效遗嘱,那么,很可能留下了财产,孩子也只能干看着!继承涉及继承人、被继承人和财产。如果作为被继承人的父母死亡,独生子女称自己是唯一合法继承人,却无法提供相关证据证明不存在其他合法继承人。

“航海——文明之迹”国际学术研讨会综述

2011年7月26—28日,在上海浦东召开了"航海——文明之迹"国际学术研讨会,本次会议由中国航海博物馆、大连海事大学主办,上海海事大学、上海海洋大学协办。研讨会共收到论文40余篇,内容涉及古船与沉船研究、海盗历史、航运贸易、海上航路、古地图、航海人物等。会议分设"古船与技术"、"海盗与人物"及"贸易与航路"三个专题,来自大连、北京、山东、上海、武汉、福建、广东。