Aim Isolation and structural elucidation of the constituents from the aerialpart of Hypericum perforatum L. Methods Column chromatography and TLC were used to isolate chemicalconstituents. Physico-chemical characteriz...Aim Isolation and structural elucidation of the constituents from the aerialpart of Hypericum perforatum L. Methods Column chromatography and TLC were used to isolate chemicalconstituents. Physico-chemical characterization and spectroscopic analysis were employed forstructural identification. Results Three flavonols were isolated and identified. Conclusion Compound1 is a novel natural product and its structure has been characterized to be 3, 5, 7-trihydroxy-3',4'-isopropyldioxy-flavone.展开更多
The roots and rhizomes of Aster tataricus L. f (Zi-wan) is a traditionalChinese medicine, which has been used for relieving cough and resolving phlegm. Previousinvestigation of the species resulted in the isolation an...The roots and rhizomes of Aster tataricus L. f (Zi-wan) is a traditionalChinese medicine, which has been used for relieving cough and resolving phlegm. Previousinvestigation of the species resulted in the isolation and identification of triterpenes,triterpenoid saponins, cyclic pentapeptides, and oligopeptides. We have also reported thepolyphenols and a dipeptide isolated from the species. In this paper, the isolation and structureelucidation of three anthraquinones, chrysophanol (1), physciori (2), and emodin (3), are described.Chrysophanol and physcion were isolated from family Composi-tae for the first time.展开更多
While conventional wastewater treatments for urban effluents are fairly routine and have proved highly effective,industrial wastewater requires more complex and specific treatments.This paper provides a technological ...While conventional wastewater treatments for urban effluents are fairly routine and have proved highly effective,industrial wastewater requires more complex and specific treatments.This paper provides a technological strategy for removal of recalcitrant contaminants based on a hybrid treatment system.The model effluent containing a binary mixture of synthetic dyes is treated by a combination of a preliminary physicochemical stage followed by a biological stage based on ligninolytic enzymes produced by Phanerochaete chrysosporium.This proposal includes biosorption onto peat as pretreatment,which decreases the volume and concentration to be treated in the biological reactor,thereby obtaining a completely decolorized effluent.The treated wastewater can therefore be reused in the dyeing baths with the consequent saving of water resources.展开更多
The preliminary purification and antimicrobial mechanism of antimicrobial peptide from Antarctic Krill were studied in this paper. The results showed that the molecular weight range of antimicrobial polypeptide (CMC...The preliminary purification and antimicrobial mechanism of antimicrobial peptide from Antarctic Krill were studied in this paper. The results showed that the molecular weight range of antimicrobial polypeptide (CMCC-1) obtained by cation exchange chromatography was between 245-709D as detected by molecular sieve chromatography, and the minimum inhibition concentration (MIC) of CMCC-1 against Staphylococcus aureus was 5.0mgmL^-1. The antimicrobial mechanism of CMCC-1 was studied with S. aureus as indicator bacterium. Compared with control group, the results of the experimental group in which S. aureus was treated with CMCC-1 were as follows: l) CMCC-1 could inhibit cell division at logarithmic phase. 2) The protein and reducing sugar con- tent, and the conductivity of culture medium increased, and the activity of alkaline phosphatase and [3-galactosidase could be detected in the culture medium. 3) Observation under scanning electron microscope revealed that somatic morphology became irregular, and then somatic surface became coarse. The cell became much smaller, and most somatic ceils gathered. The boundary between cells became dim and finally fused as a whole. 4) Observation under transmission electron microscope showed that the surface of S. aureus became rough and the reproducing ability was restrained. The cell wall became thin and the cytoplasm shrunk. Substances inside cell leaked out, which caused cells death. 5) SDS-PAGE analysis showed that some bands disappeared, and the residual bands became vague. 6) The genomic DNA electrophoresis results showed that the genomic DNA bands ofS. aureus were not degraded but the brightness significantly reduced. Thus, it is supposed that CMCC-1 could destroy the cell wall and membrane of S. aureu, increase the cell membrane permeability and the leaking-out of intracellular substances, and thus cause the death ofS. aureu.展开更多
An efficient method for quality control of Fructus Aurantii Immaturus (FAI),a famous traditional Chinese medicine (TCM) was established. A simple and reliable high-performance liquid chromatography-photodiode array de...An efficient method for quality control of Fructus Aurantii Immaturus (FAI),a famous traditional Chinese medicine (TCM) was established. A simple and reliable high-performance liquid chromatography-photodiode array detector (HPLC-DAD) procedure coupled with chemometric methods was developed for fingerprint analysis,qualitative analysis and quantitative determination of this herb. In qualitative and quantitative analyses,heuristic evolving latent projection (HELP) method was employed to resolve the overlapping peaks of the tested samples. Two bioactive components,namely hesperidin and naringin,are confirmed and determined,together with four flavonoids compounds tentatively identified including two new ones. From fingerprint analysis,the fingerprint data were processed with correlation coefficients for quantitative expression of their similarity and dissimilarity. The developed method based on an integration of chromatographic fingerprint and quantitative analysis is scientific,and the obtained results can be applied to the quality control of herb medicine.展开更多
Rhodotorula sp. NJ298 which could produce carotenoids was isolated from Antarctic sea ice. The major carotenoid was identified as astaxanthin by Liquid Chromatography/Mass Spectrometry (LC/MS), and its content accou...Rhodotorula sp. NJ298 which could produce carotenoids was isolated from Antarctic sea ice. The major carotenoid was identified as astaxanthin by Liquid Chromatography/Mass Spectrometry (LC/MS), and its content accounted for 87.62% of total carotenoids (1,786 μg/g). High Performance Liquid Chromatogrephy (HPLC) analysis showed that the purity of the astaxanthin reached about 96. 16% through a simple purification. Maximum astaxanthin production (1,908μg/g) was obtained when the yeast was grown at 10 ℃ in seawater medium containing 5 g/L sodium acetate, 5 g/L peptone, 0.5 g/L NaC1, 0.01 g/L KH2PO4; 0.01 g/L MgSO4·7H20 and 0.001 g/L FeSO4·7H20 at pH 7.5.展开更多
Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), a...Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).展开更多
[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana( L. ) R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety (white violet), Franc...[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana( L. ) R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety (white violet), Francesco variety ( red violet) and Arabella variety ( purple violet) were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows: with 95% ethanol + 5% HCI as solvent, solid-liquid ratio ( 1 : 40), extraction time (3 h), extraction temperature (65 ℃ ). The opti- mum extraction conditions of red violet and purple violet were as follows: with acetone as solvent, solid-liquid ratio (1:40), extraction time (3 h), extraction temperature (65 ℃). [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana( L. ) R. Br.展开更多
The family of Cr(Ⅲ) and Fe(Ⅲ)-doped rutile pigments of nominal composition (M^ⅢM^V)xTi1-2xO2, with M^Ⅲ = Cr(Ⅲ) or Fe(Ⅲ) and M'(V) = Sb, Nb, Ta, with x = 0.03, 0.15 and 0.25 were investigated as cera...The family of Cr(Ⅲ) and Fe(Ⅲ)-doped rutile pigments of nominal composition (M^ⅢM^V)xTi1-2xO2, with M^Ⅲ = Cr(Ⅲ) or Fe(Ⅲ) and M'(V) = Sb, Nb, Ta, with x = 0.03, 0.15 and 0.25 were investigated as ceramic pigments covering the yellow-ochre-brown palette. The formulations containing Fe(Ⅲ) are novel compositions not included in the commercial rutile pigments. The materials were characterized by XRD (X-ray diffraction) analysis and FTIR (Fourier transformed infrared spectroscopy). The transition temperature from anatase-to-rutile was estimated by the evolution of the spectral patterns. This crystal phase transition is responsible of the color formation. A higher distortion of TiO6 octahedra is observed in the case of (FeSb) containing cells which contribute to the enhancement of the light absorption. The coloring performance of all the formulations were evaluated by enameling the mixtures containing 5% pigments and commercial frits representative of single and double firing industrial processes. The chromatic values obtained are in the yellow to brown domain of the chromatic plot, depending on the composition of the pigment-frit batch. In the case of the Fe-glazes, and particularly the combination (FeNb), the chromatic values are close to the best yellow tinting. This new FeNb-rutile pigment could be a more benign substitute of Cr-yellow pigments. The homogeneity of the enamels was confirmed by SEM (scanning electron microscopy)-EDAX (energy dispersive X-ray analysis) microscopy.展开更多
Compounds isolated and identified in a culture of the alga-endophytic fungus Talaromyces sp. cf- 16 included two naturally occurring alkaloids, 2-[(S)-hydroxy(phenyl)methyl]-3-mcthylquinazolin-4(3H)- one (la) ...Compounds isolated and identified in a culture of the alga-endophytic fungus Talaromyces sp. cf- 16 included two naturally occurring alkaloids, 2-[(S)-hydroxy(phenyl)methyl]-3-mcthylquinazolin-4(3H)- one (la) and 2-[(R)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (lb), that were identified for the first time. In addition, seven known compounds (2-8) were obtained from the culture. Following chiral column chromatography, compounds la and lb were identified as enantiomers by spectroscopic analyses and quantum chemical calculations. Bioassay results showed that 5 was more toxic to brine shrimp than the other compounds, and that 3-6 could inhibit Staphylococcus aureus.展开更多
The aim of this work was to focus on development of the new health benefit food product, bread made from colored wheat flour with a high content of proteins, fibre, β-glucans, phenols, flavonoids. The amount of prote...The aim of this work was to focus on development of the new health benefit food product, bread made from colored wheat flour with a high content of proteins, fibre, β-glucans, phenols, flavonoids. The amount of protein ranged from 9% to 14% in our analyzed set. The highest value had yellow variety of wheat BONA DEA (14%), at least had red grain of variety FEDERER (9.02%). Average content of fl-glucans determined by enzymatically ranged from 0.25% (BONA VITA) to 0.67% (ABISSINSKAJA and LUTEUS). The largest amount of phenolic compounds (134 mg GAE/L) and flavonoids (2.80 mg RE/L) contained sample RU440-6, the lowest sample was IS CORVINUS (87 mg GAE/L). The highest antioxidant activity was observed in blue varieties of wheat 48M (0.269 mmol TE/L), while the lowest was determined in a sample of purple ANK 28A (0.083 mmol TE/L). Data show that there is potential to use purple, blue and red variety of wheat as novel ingredient resources for the development of new innovative products. Assessment of the results we selected two varieties of color wheat (KONINI, RU440-6) in bakery attempt to meet demand for nutritional quality and also have an increased content of healthy components. Overall the most pleasant taste, color and flexibility bread crumb had a loaf of bread prepared from a purple variety KONINI without the addition of β-glucans.展开更多
Seaweeds are known to exhibit various antimicrobial properties, since it harbours an enormous range of indigenous bioactive compounds. The emergence of drug resistant strains has directed to the identification of pros...Seaweeds are known to exhibit various antimicrobial properties, since it harbours an enormous range of indigenous bioactive compounds. The emergence of drug resistant strains has directed to the identification of prospective metabolites from seaweed and its endophytes, thereby exploiting the properties in resisting bacterial diseases. The current study was aimed to assess the antimicrobial activity of extracts obtained from Ulva reticulate, for which metabolites of Ulva reticulata and its endophytes were extracted and assessed against human pathogens like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Bacillus subtilis. It was observed that the hexane extract of isolate VITDSJ2 was effective against all the tested pathogens but a significant inhibition was observed for Staphylococcus aureus and Escherichia coli. Further, Gas chromatography coupled with Mass spectroscopy (GC-MS) revealed the existence of phenol, 3, 5-bis (1, 1-dimethylethyl) in the crude hexane extract which is well-known to possess antibacterial activity. The effective isolate VITDSJ2 was identified to be the closest neighbour of Pseudomonas smtzeri by phenotypic and genotypic methods. The crude extracts of the seaweed Ulva reticulata was also screened for antibacterial activity and the hexane extract was effective in showing inhibition against all the tested pathogens. The compound in the crude extract of Ulva reticulata was identified as hentriacontane using GC-MS. The extracts obtained from dichloromethane did not show significant activity in comparison with the hexane extracts. Hence the metabolites of Ulva reticulata and the bacterial secondary metabolites of the endophytes could be used in the treatment of bacterial infections.展开更多
The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator....The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator. The extracts were tested for content of the following phytochemicals using standard methods: alkaloids, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and steroids. Antibacterial sensitivity test of the extract was carried out on S. aureus, B. subtilis and E. coli using agar well diffusion method and Ampiclox as control. The effect of storage time of the extract on its antibacterial activity was determined after the extract was kept at room temperature for 1 h, 24 h, 48 h and 72 h, respectively. Preparations of 200 mg/mL of the aqueous extract were made with additives as follows: (a) no additive; (b) methyl paraben (0.3%); (c) propyl paraben (0.3%); (d) sodium metabisulfite (0.01%); (e) A combination of methyl paraben (0.3%) and propyl paraben (0.3%); (f) A combination of methyl paraben (0.3%), propyl paraben (0.3%) and sodium metabisulfite (0.01%). A visual observation of the stabilized preparations was employed on daily basis for 30 days. Phytochemical analyses on the aqueous extract of the fresh leaves showed the absence of alkaloids, anthraquinones and flavonoids. Saponins, cardiac glycosides and steroids were present in moderate amounts and there was abundance of tannins. The extract had similar effect on both S. aureus and E. coli cultures but activity was very low with B. subtilis. When S. aureus activity increased with time of storage up to 48 hours, thereafter the activity started reducing. However, the activity of E. coli continued to increase with time (within the study period). Thus, it appears reasonable to store the extract for at least 48 hours before the use for enhanced activity.展开更多
The southern region of the state of Chihuahua is mining by origin, as this has been proved ghost towns of Minas Nuevas, it is also called Villa Escobedo, Emerald and Blue. Minerals are nonrenewable resources. There ar...The southern region of the state of Chihuahua is mining by origin, as this has been proved ghost towns of Minas Nuevas, it is also called Villa Escobedo, Emerald and Blue. Minerals are nonrenewable resources. There are only reserves and waste deposited in tailings ponds of low-rade precious metals (gold and silver). A conventional method for recovering gold and silver ore is low-grade cyanidation and the solvent consider toxic and highly dangerous for the flora, fauna, humans and the environment. The mechanism of cyanide poisoning is inhibition of cytochrome oxidase--an enzyme needed for cell respiration. Without compromising efficiency, sensitivity and cost of the process, the toxic solvent is replaced by a less aggressive one, which helps the environment, public and occupational health. (NH4)2S203 has no appreciable damage to conservative amounts, is a selective aqueous solvent for leaching gold and silver, easy to get and manipulate. This work is done in the Technological Institute of Parral in a low-grade ore from the southern region of the state of Chihuahua. This document has been enriched presented in the IMEC 2014, with the increase of series Ⅴ and Ⅵ.展开更多
Antimicrobial and photocatalytic effect of silicate and silicone paints were tested. Silicate paints are based on water styrene-acrylic dispersion with mineral binder into form of potassium water glass. Silicone paint...Antimicrobial and photocatalytic effect of silicate and silicone paints were tested. Silicate paints are based on water styrene-acrylic dispersion with mineral binder into form of potassium water glass. Silicone paints are based on water acrylic dispersion with silicone resin emulsion. Silicate and silicone paints were formulated with photocatalytic active nanooxides TiO2 and ZnO. Photocatalytic efficiency of coatings was measured as an absorbance change of organic dye Orange II solution at a wavelength of 485 nm. Antimicrobial properties of coatings were tested using agar plate methods. As test microorganisms Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Penicillium chrysogenum and Aspergillus niger suspension (density 10^6 cells/mL) were used. The inhibition effect of tested coatings and inhibitory zones were evaluated after incubation. Coatings containing nanoparticles of titanium dioxide and zinc oxide showed antimicrobial activity against the test microorganisms. Whereas coating with zinc oxide nanoparticles successfully inhibited the growth of both bacteria and fungi, the photocatalytic nanoparticles of titanium dioxide inhibited bacteria but not fungi (weak inhibition was observed).展开更多
文摘Aim Isolation and structural elucidation of the constituents from the aerialpart of Hypericum perforatum L. Methods Column chromatography and TLC were used to isolate chemicalconstituents. Physico-chemical characterization and spectroscopic analysis were employed forstructural identification. Results Three flavonols were isolated and identified. Conclusion Compound1 is a novel natural product and its structure has been characterized to be 3, 5, 7-trihydroxy-3',4'-isopropyldioxy-flavone.
文摘The roots and rhizomes of Aster tataricus L. f (Zi-wan) is a traditionalChinese medicine, which has been used for relieving cough and resolving phlegm. Previousinvestigation of the species resulted in the isolation and identification of triterpenes,triterpenoid saponins, cyclic pentapeptides, and oligopeptides. We have also reported thepolyphenols and a dipeptide isolated from the species. In this paper, the isolation and structureelucidation of three anthraquinones, chrysophanol (1), physciori (2), and emodin (3), are described.Chrysophanol and physcion were isolated from family Composi-tae for the first time.
基金Supported by the Proyecto Fondecyt (1040089,1090098)
文摘While conventional wastewater treatments for urban effluents are fairly routine and have proved highly effective,industrial wastewater requires more complex and specific treatments.This paper provides a technological strategy for removal of recalcitrant contaminants based on a hybrid treatment system.The model effluent containing a binary mixture of synthetic dyes is treated by a combination of a preliminary physicochemical stage followed by a biological stage based on ligninolytic enzymes produced by Phanerochaete chrysosporium.This proposal includes biosorption onto peat as pretreatment,which decreases the volume and concentration to be treated in the biological reactor,thereby obtaining a completely decolorized effluent.The treated wastewater can therefore be reused in the dyeing baths with the consequent saving of water resources.
基金supported by the National Science and Technology Pillar Program(2013BAD13B03)National Natural Science Foundation of China(Grant 31201311)Special Scientific Research Funds for Central Non-profit Institutes,Yellow Sea Fisheries Research Institute(20603022012001)
文摘The preliminary purification and antimicrobial mechanism of antimicrobial peptide from Antarctic Krill were studied in this paper. The results showed that the molecular weight range of antimicrobial polypeptide (CMCC-1) obtained by cation exchange chromatography was between 245-709D as detected by molecular sieve chromatography, and the minimum inhibition concentration (MIC) of CMCC-1 against Staphylococcus aureus was 5.0mgmL^-1. The antimicrobial mechanism of CMCC-1 was studied with S. aureus as indicator bacterium. Compared with control group, the results of the experimental group in which S. aureus was treated with CMCC-1 were as follows: l) CMCC-1 could inhibit cell division at logarithmic phase. 2) The protein and reducing sugar con- tent, and the conductivity of culture medium increased, and the activity of alkaline phosphatase and [3-galactosidase could be detected in the culture medium. 3) Observation under scanning electron microscope revealed that somatic morphology became irregular, and then somatic surface became coarse. The cell became much smaller, and most somatic ceils gathered. The boundary between cells became dim and finally fused as a whole. 4) Observation under transmission electron microscope showed that the surface of S. aureus became rough and the reproducing ability was restrained. The cell wall became thin and the cytoplasm shrunk. Substances inside cell leaked out, which caused cells death. 5) SDS-PAGE analysis showed that some bands disappeared, and the residual bands became vague. 6) The genomic DNA electrophoresis results showed that the genomic DNA bands ofS. aureus were not degraded but the brightness significantly reduced. Thus, it is supposed that CMCC-1 could destroy the cell wall and membrane of S. aureu, increase the cell membrane permeability and the leaking-out of intracellular substances, and thus cause the death ofS. aureu.
基金Project(20875104) supported by the National Natural Science Foundation of ChinaProject(10SDF22) supported by the Special Foundation of China Postdoctoral ScienceProject(201021200011) supported by the Advanced Research Plan of Central South University, China
文摘An efficient method for quality control of Fructus Aurantii Immaturus (FAI),a famous traditional Chinese medicine (TCM) was established. A simple and reliable high-performance liquid chromatography-photodiode array detector (HPLC-DAD) procedure coupled with chemometric methods was developed for fingerprint analysis,qualitative analysis and quantitative determination of this herb. In qualitative and quantitative analyses,heuristic evolving latent projection (HELP) method was employed to resolve the overlapping peaks of the tested samples. Two bioactive components,namely hesperidin and naringin,are confirmed and determined,together with four flavonoids compounds tentatively identified including two new ones. From fingerprint analysis,the fingerprint data were processed with correlation coefficients for quantitative expression of their similarity and dissimilarity. The developed method based on an integration of chromatographic fingerprint and quantitative analysis is scientific,and the obtained results can be applied to the quality control of herb medicine.
基金Supported by the National Natural Science Foundation of China (No. 40406003 and 40206022).
文摘Rhodotorula sp. NJ298 which could produce carotenoids was isolated from Antarctic sea ice. The major carotenoid was identified as astaxanthin by Liquid Chromatography/Mass Spectrometry (LC/MS), and its content accounted for 87.62% of total carotenoids (1,786 μg/g). High Performance Liquid Chromatogrephy (HPLC) analysis showed that the purity of the astaxanthin reached about 96. 16% through a simple purification. Maximum astaxanthin production (1,908μg/g) was obtained when the yeast was grown at 10 ℃ in seawater medium containing 5 g/L sodium acetate, 5 g/L peptone, 0.5 g/L NaC1, 0.01 g/L KH2PO4; 0.01 g/L MgSO4·7H20 and 0.001 g/L FeSO4·7H20 at pH 7.5.
基金Supported by the Ministry of Science and Technology(No.2010CB833802)the Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-EW-G-12B)the National Natural Science Foundation of China(No.30910103914)
文摘Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).
基金Supported by Natural Science Foundation in Chongqing(CSTC,2006BB1336)Applied Basic Research Program of Chongqing Education Department(KJ060308)
文摘[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana( L. ) R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety (white violet), Francesco variety ( red violet) and Arabella variety ( purple violet) were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows: with 95% ethanol + 5% HCI as solvent, solid-liquid ratio ( 1 : 40), extraction time (3 h), extraction temperature (65 ℃ ). The opti- mum extraction conditions of red violet and purple violet were as follows: with acetone as solvent, solid-liquid ratio (1:40), extraction time (3 h), extraction temperature (65 ℃). [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana( L. ) R. Br.
文摘The family of Cr(Ⅲ) and Fe(Ⅲ)-doped rutile pigments of nominal composition (M^ⅢM^V)xTi1-2xO2, with M^Ⅲ = Cr(Ⅲ) or Fe(Ⅲ) and M'(V) = Sb, Nb, Ta, with x = 0.03, 0.15 and 0.25 were investigated as ceramic pigments covering the yellow-ochre-brown palette. The formulations containing Fe(Ⅲ) are novel compositions not included in the commercial rutile pigments. The materials were characterized by XRD (X-ray diffraction) analysis and FTIR (Fourier transformed infrared spectroscopy). The transition temperature from anatase-to-rutile was estimated by the evolution of the spectral patterns. This crystal phase transition is responsible of the color formation. A higher distortion of TiO6 octahedra is observed in the case of (FeSb) containing cells which contribute to the enhancement of the light absorption. The coloring performance of all the formulations were evaluated by enameling the mixtures containing 5% pigments and commercial frits representative of single and double firing industrial processes. The chromatic values obtained are in the yellow to brown domain of the chromatic plot, depending on the composition of the pigment-frit batch. In the case of the Fe-glazes, and particularly the combination (FeNb), the chromatic values are close to the best yellow tinting. This new FeNb-rutile pigment could be a more benign substitute of Cr-yellow pigments. The homogeneity of the enamels was confirmed by SEM (scanning electron microscopy)-EDAX (energy dispersive X-ray analysis) microscopy.
基金Supported by the National Key Technology Research and Development Program for Agriculture(No.2012BAD32B09)the National Natural Science Foundation of China(Nos.41106137,41106136)+1 种基金the Taishan Scholar Program of Shandong Provincethe Chinese Academy of Sciences for Key Topics in Innovation Engineering(No.KZCX2-YW-QN209)
文摘Compounds isolated and identified in a culture of the alga-endophytic fungus Talaromyces sp. cf- 16 included two naturally occurring alkaloids, 2-[(S)-hydroxy(phenyl)methyl]-3-mcthylquinazolin-4(3H)- one (la) and 2-[(R)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (lb), that were identified for the first time. In addition, seven known compounds (2-8) were obtained from the culture. Following chiral column chromatography, compounds la and lb were identified as enantiomers by spectroscopic analyses and quantum chemical calculations. Bioassay results showed that 5 was more toxic to brine shrimp than the other compounds, and that 3-6 could inhibit Staphylococcus aureus.
文摘The aim of this work was to focus on development of the new health benefit food product, bread made from colored wheat flour with a high content of proteins, fibre, β-glucans, phenols, flavonoids. The amount of protein ranged from 9% to 14% in our analyzed set. The highest value had yellow variety of wheat BONA DEA (14%), at least had red grain of variety FEDERER (9.02%). Average content of fl-glucans determined by enzymatically ranged from 0.25% (BONA VITA) to 0.67% (ABISSINSKAJA and LUTEUS). The largest amount of phenolic compounds (134 mg GAE/L) and flavonoids (2.80 mg RE/L) contained sample RU440-6, the lowest sample was IS CORVINUS (87 mg GAE/L). The highest antioxidant activity was observed in blue varieties of wheat 48M (0.269 mmol TE/L), while the lowest was determined in a sample of purple ANK 28A (0.083 mmol TE/L). Data show that there is potential to use purple, blue and red variety of wheat as novel ingredient resources for the development of new innovative products. Assessment of the results we selected two varieties of color wheat (KONINI, RU440-6) in bakery attempt to meet demand for nutritional quality and also have an increased content of healthy components. Overall the most pleasant taste, color and flexibility bread crumb had a loaf of bread prepared from a purple variety KONINI without the addition of β-glucans.
文摘Seaweeds are known to exhibit various antimicrobial properties, since it harbours an enormous range of indigenous bioactive compounds. The emergence of drug resistant strains has directed to the identification of prospective metabolites from seaweed and its endophytes, thereby exploiting the properties in resisting bacterial diseases. The current study was aimed to assess the antimicrobial activity of extracts obtained from Ulva reticulate, for which metabolites of Ulva reticulata and its endophytes were extracted and assessed against human pathogens like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Bacillus subtilis. It was observed that the hexane extract of isolate VITDSJ2 was effective against all the tested pathogens but a significant inhibition was observed for Staphylococcus aureus and Escherichia coli. Further, Gas chromatography coupled with Mass spectroscopy (GC-MS) revealed the existence of phenol, 3, 5-bis (1, 1-dimethylethyl) in the crude hexane extract which is well-known to possess antibacterial activity. The effective isolate VITDSJ2 was identified to be the closest neighbour of Pseudomonas smtzeri by phenotypic and genotypic methods. The crude extracts of the seaweed Ulva reticulata was also screened for antibacterial activity and the hexane extract was effective in showing inhibition against all the tested pathogens. The compound in the crude extract of Ulva reticulata was identified as hentriacontane using GC-MS. The extracts obtained from dichloromethane did not show significant activity in comparison with the hexane extracts. Hence the metabolites of Ulva reticulata and the bacterial secondary metabolites of the endophytes could be used in the treatment of bacterial infections.
文摘The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator. The extracts were tested for content of the following phytochemicals using standard methods: alkaloids, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and steroids. Antibacterial sensitivity test of the extract was carried out on S. aureus, B. subtilis and E. coli using agar well diffusion method and Ampiclox as control. The effect of storage time of the extract on its antibacterial activity was determined after the extract was kept at room temperature for 1 h, 24 h, 48 h and 72 h, respectively. Preparations of 200 mg/mL of the aqueous extract were made with additives as follows: (a) no additive; (b) methyl paraben (0.3%); (c) propyl paraben (0.3%); (d) sodium metabisulfite (0.01%); (e) A combination of methyl paraben (0.3%) and propyl paraben (0.3%); (f) A combination of methyl paraben (0.3%), propyl paraben (0.3%) and sodium metabisulfite (0.01%). A visual observation of the stabilized preparations was employed on daily basis for 30 days. Phytochemical analyses on the aqueous extract of the fresh leaves showed the absence of alkaloids, anthraquinones and flavonoids. Saponins, cardiac glycosides and steroids were present in moderate amounts and there was abundance of tannins. The extract had similar effect on both S. aureus and E. coli cultures but activity was very low with B. subtilis. When S. aureus activity increased with time of storage up to 48 hours, thereafter the activity started reducing. However, the activity of E. coli continued to increase with time (within the study period). Thus, it appears reasonable to store the extract for at least 48 hours before the use for enhanced activity.
文摘The southern region of the state of Chihuahua is mining by origin, as this has been proved ghost towns of Minas Nuevas, it is also called Villa Escobedo, Emerald and Blue. Minerals are nonrenewable resources. There are only reserves and waste deposited in tailings ponds of low-rade precious metals (gold and silver). A conventional method for recovering gold and silver ore is low-grade cyanidation and the solvent consider toxic and highly dangerous for the flora, fauna, humans and the environment. The mechanism of cyanide poisoning is inhibition of cytochrome oxidase--an enzyme needed for cell respiration. Without compromising efficiency, sensitivity and cost of the process, the toxic solvent is replaced by a less aggressive one, which helps the environment, public and occupational health. (NH4)2S203 has no appreciable damage to conservative amounts, is a selective aqueous solvent for leaching gold and silver, easy to get and manipulate. This work is done in the Technological Institute of Parral in a low-grade ore from the southern region of the state of Chihuahua. This document has been enriched presented in the IMEC 2014, with the increase of series Ⅴ and Ⅵ.
文摘Antimicrobial and photocatalytic effect of silicate and silicone paints were tested. Silicate paints are based on water styrene-acrylic dispersion with mineral binder into form of potassium water glass. Silicone paints are based on water acrylic dispersion with silicone resin emulsion. Silicate and silicone paints were formulated with photocatalytic active nanooxides TiO2 and ZnO. Photocatalytic efficiency of coatings was measured as an absorbance change of organic dye Orange II solution at a wavelength of 485 nm. Antimicrobial properties of coatings were tested using agar plate methods. As test microorganisms Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Penicillium chrysogenum and Aspergillus niger suspension (density 10^6 cells/mL) were used. The inhibition effect of tested coatings and inhibitory zones were evaluated after incubation. Coatings containing nanoparticles of titanium dioxide and zinc oxide showed antimicrobial activity against the test microorganisms. Whereas coating with zinc oxide nanoparticles successfully inhibited the growth of both bacteria and fungi, the photocatalytic nanoparticles of titanium dioxide inhibited bacteria but not fungi (weak inhibition was observed).