正品黄芩与甘肃黄芩有效化学成分及抑菌作用的比较研究

目的:通过对甘肃黄芩与正品黄岑的有效化学成分(黄芩甙、黄芩素、汉黄芩素)的含量及二者的生物活性比较研究,评价甘肃黄芩的药材质量。方法:RP-HPLC法测定有效化学成分;管碟法测定两种黄芪的抑菌效力。结果:黄芩甙、黄芩素、汉黄芩素的线性范围分别为10～200μg、5～100μg和5～50μg,平均回收率分别为99.45%(RSD=0.54%)、97.36%(RSD=1.38%)、98.64%(RSD=1.19%)。甘肃黄芩与正品黄芩比较,黄芩甙含量基本一致。6月份采挖的甘肃黄芩的黄芩甙含量最高。甘肃黄芩的抑菌力为正品黄芩的82.03%。结论:甘肃黄芩的黄芩甙含量和抑菌效果接近正品黄芩,具有代替正品黄芩的物质基础。

复方黄芩片质量标准的研究

目的建立复方黄芩片的质量控制标准。方法采用薄层色谱法对复方黄芩片中的黄芩、虎杖、功劳木等进行了定性鉴别。采用HPLC法对制剂中的黄芩苷进行了含量测定。流动相:甲醇-0.4%磷酸(50∶50),流速:1.4mL/min;柱温:40℃;检测波长:278nrn。结果鉴别项下阴性对照无干扰,专属性强;黄芩苷在0.12～0.72μg范围内有良好线性关系(r=0.9996),平均回收率为97.17%,RSD=1.78%(n=5)。结论该方法可为复方黄芩片的质量评价提供科学依据。

中药复方制剂中黄芩苷转移率影响因素研究

目的:考察不同剂型及其不同工序对黄芩苷转移率的影响方法:采用转移率测定法以黄芩苷的转移率为指标进行试验。结果:水提醇沉法优于醇提法加火单黄芩的转移率优于未加火单黄芩,浓缩过程极大的降低黄芩苷的转移率。结论:中药复方制剂中黄芩苷转移率的影响因素有提取方法、浓缩工艺以及黄芩的炮制方法等。

生黄芩与酒黄芩不同提取方法对黄芩苷煎出量的影响

目的:研究生黄芩与酒黄芩用不同提取方法对有效成分黄芩甙煎出量的影响。方法:对生黄芩与酒黄芩分别用热冷水投料煎煮,用高效液相色谱法测定煎煮液中黄芩甙含量,将相关数据进行对比。结果:在制剂时,酒黄芩冷水投料后煎煮液中黄芩甙没有过多损失。结论:在制剂中,酒黄芩可用冷水投料。

HPLC法测定粉痤胶囊中黄芩苷的含量

目的:用HPLC法测定粉痤胶囊中黄芩苷的含量。方法:色谱柱为Kromasil C18柱,以甲醇-乙腈-水-磷酸(20:20:60:0.15)为流动相,检测波长280 nm。结果:黄芩苷在4.9～78.4μg·ml-1范围内呈良性线形关系,r=0.9998,平均回收率为98.3%。结论:该方法专属性强,结果准确可靠,适于粉痤胶囊的质量控制。

消痤胶囊的质量控制

目的:建立消痤胶囊的质量控制标准。方法:采用薄层色谱法对消痤胶囊中的连翘、大黄进行定性鉴别;用高效液相色谱法对制剂中的黄芩苷的含量进行测定。结果:薄层色谱斑点清晰,专属性强;定量分析黄芩苷线性范围为A=20568X-163,r=0.9997,平均回收98.67%,RSD=1.41%。结论:所选用的定性定量方法简便准确,可用于该制剂的质量控制。

Measurement and Correlation of Partition Coefficients of Baicalin in EOPO/Salt Aqueous Two-Phase Systems

The partition coefficients of baicalin were measured in ethylene oxide and propylene oxide (EOPO)/salt aqueous two-phase systems at 298.15K. It was found that most of baicalin partitioned into EOPO-rich phase. The partition coefficients of baicalin varied from 10 to 120. The effect of various factors, including tie-line length, salt composition, molecular weight of EOPO, and solution pH, on the partition behavior was investigated in EOPO/salt systems. Furthermore the partition coefficients of baicalin were correlated using the modified Diamond-Hsu model. Good agreement with experimental data is obtained. The average relative deviations are less than 5.0%.

Effects of ecological factors on secondary metabolites and inorganic elements of Scutellaria baicalensis and analysis of geoherblism

This study analyzed the effects of ecological factors on secondary metabolites of Scutellaria baicalensis using two sources:92individual roots of S.baicalensis from all over China,and secondary metabolites,medicinal materials and inorganic element contents obtained from the testing of 92 S.baicalensis rhizosphere soil samples.The study used environmental data from the Genuine Medicinal Material Spatial Analysis Database.Most of the chemical constituents of S.baicalensis were negatively correlated to latitude and positively correlated to temperature;generally,the contents of 21 chemical constituents were higher at low latitudes than that at high latitudes.By gradual regression analysis,it was found that the content of baicalin in S.baicalensis was negatively correlated to latitude and generally the content of inorganic elements in soil was excessively high(excluding Mg and Ca),which has a negative effect on the accumulation of chemical constituents in S.baicalensis.Based on the cluster analysis of 21 constituents,S.baicalensis from different places of origin was divided into two groups,and S.baicalensis was not genuine only in a specific small region.Within the zone from Chifeng,Inner Mongolia to Taibai,Shaanxi is suitable for accumulation of secondary metabolites of S.baicalensis and such a zone represents a suitable distribution and potential genuine producing area.