目的:通过系统评价及Meta分析,评价黄芩类制剂局部应用辅助牙周基础治疗对慢性牙周炎的临床疗效。方法:检索Pubmed、Cochrane Library、Web of Knowledge、CNKI、CBM数据库,查找黄芩制剂辅助牙周基础治疗局部应用于慢性牙周炎的随机对...目的:通过系统评价及Meta分析,评价黄芩类制剂局部应用辅助牙周基础治疗对慢性牙周炎的临床疗效。方法:检索Pubmed、Cochrane Library、Web of Knowledge、CNKI、CBM数据库,查找黄芩制剂辅助牙周基础治疗局部应用于慢性牙周炎的随机对照实验。资料提取和质量评价后,选取空白对照研究,对探诊深度、临床附着丧失的改变按照用药方法的不同分亚组进行Meta定量分析,对其他临床指标进行系统评价。结果:共9篇文章、652例受试者纳入本研究。结果显示:黄芩类制剂的局部辅助治疗慢性牙周炎,在降低探诊深度[MD=-1.49,95%CI(-2.15,-0.38),P<0.00001],改善牙龈出血及细菌学指标方面,疗效优于单纯基础治疗,未出现明显临床不适;对于附着丧失的改善,有待观察[MD=-0.32,95%CI(-1.01,0.38),P=0.03]。结论:黄芩类制剂局部辅助慢性牙周炎的治疗,是有效的手段。展开更多
The present study aimed at identifying potential lead compounds for diabetes mellitus drug discovery. We developed a novel method involving centrifugal ultrafiltration separation subsequent liquid chromatography with ...The present study aimed at identifying potential lead compounds for diabetes mellitus drug discovery. We developed a novel method involving centrifugal ultrafiltration separation subsequent liquid chromatography with quadrupole time of flight tandem mass spectrometry （LC-Q/TOF-MS/MS） determination to screen a-glucosidase inhibitors in complex Scutellaria baicalensis Georgi （SBG） extract. By adding a second filter to the screening process, the level of non-specific binding of Compounds 1, 3, 10 and 11 was significantly decreased, and the level of non-specific binding of Compounds 5 and 15 also was reduced. As a result, five flavonoids identified as baicalein, baicalein, wogonin, chrysin, and oroxylin A, were rapidly found to interact with α-glucosidase and possess potent anti-a-glueosidase aetivity in vitro. Specific binding of ligands to a-glucosidase was demonstrated though the proposed method and the ligands could be ranked in order of affinity for α-glucosidase, which were corresponded to the order of inhibitory activity in vitro. In conclusion, our results indicated that the developed method is a rapid and effective screening method for rat intestinal α-glucosidase inhibitors from complex herbal medicines such as SBG.展开更多
文摘目的:通过系统评价及Meta分析,评价黄芩类制剂局部应用辅助牙周基础治疗对慢性牙周炎的临床疗效。方法:检索Pubmed、Cochrane Library、Web of Knowledge、CNKI、CBM数据库,查找黄芩制剂辅助牙周基础治疗局部应用于慢性牙周炎的随机对照实验。资料提取和质量评价后,选取空白对照研究,对探诊深度、临床附着丧失的改变按照用药方法的不同分亚组进行Meta定量分析,对其他临床指标进行系统评价。结果:共9篇文章、652例受试者纳入本研究。结果显示:黄芩类制剂的局部辅助治疗慢性牙周炎,在降低探诊深度[MD=-1.49,95%CI(-2.15,-0.38),P<0.00001],改善牙龈出血及细菌学指标方面,疗效优于单纯基础治疗,未出现明显临床不适;对于附着丧失的改善,有待观察[MD=-0.32,95%CI(-1.01,0.38),P=0.03]。结论:黄芩类制剂局部辅助慢性牙周炎的治疗,是有效的手段。
基金supported by the National Key Scientific and Technological Special Projects(2012ZX09103-101-007)the Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT-IRT1193)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘The present study aimed at identifying potential lead compounds for diabetes mellitus drug discovery. We developed a novel method involving centrifugal ultrafiltration separation subsequent liquid chromatography with quadrupole time of flight tandem mass spectrometry （LC-Q/TOF-MS/MS） determination to screen a-glucosidase inhibitors in complex Scutellaria baicalensis Georgi （SBG） extract. By adding a second filter to the screening process, the level of non-specific binding of Compounds 1, 3, 10 and 11 was significantly decreased, and the level of non-specific binding of Compounds 5 and 15 also was reduced. As a result, five flavonoids identified as baicalein, baicalein, wogonin, chrysin, and oroxylin A, were rapidly found to interact with α-glucosidase and possess potent anti-a-glueosidase aetivity in vitro. Specific binding of ligands to a-glucosidase was demonstrated though the proposed method and the ligands could be ranked in order of affinity for α-glucosidase, which were corresponded to the order of inhibitory activity in vitro. In conclusion, our results indicated that the developed method is a rapid and effective screening method for rat intestinal α-glucosidase inhibitors from complex herbal medicines such as SBG.
