益髓颗粒中黄芪甲苷的含量测定研究

目的：建立益髓颗粒中黄芪甲苷的含量测定方法。方法：采用C18柱（CAPCELL PKA MG ，4．6mm ×250mm ，5μm ），柱温：35℃，乙腈：水（35：65）为流动相，ELSD检测器漂移管温度90℃，气体流速：3．0L · min-1。结果：黄芪甲苷在0．8992～5．62μg呈良好的线性关系（r ＝0．9997），平均回收率为94．9％，R S D为2．19％。结论：该方法准确，重现性较好，可作为益髓颗粒质量控制方法。

HPLC-ELSD法测定芪风颗粒中黄芪甲苷的含量

目的：建立高效液相色谱-蒸发光散射检测法（HPLC-ELSD）测定芪风颗粒中黄芪甲苷的含量。方法：采用HPLC-ELSD法,以黄芪甲苷为对照品对芪风颗粒中的黄芪甲苷进行定量分析。流动相：乙腈-水（32∶68）;流速：1 mL.min-1;进样量：50μL;蒸发光散射检测器：漂移管温度为70℃,载气流速为25psi。结果：黄芪甲苷在1.01~20.19μg范围内线性良好,平均回收率（n=9）为99.4%,RSD为3.2%。结论：采用HPLC-ELSD法测定芪风颗粒中黄芪甲苷的含量方法简便,重现性好,结果准确。

牙周康泰胶囊质量标准研究

目的 建立牙周康泰胶囊的质量标准。方法 采用薄层色谱法对牙周康泰胶囊中丹参、黄芪、金银花、黄柏进行定性鉴别,采用双波长薄层扫描法测定君药山茱萸中主要化学成分熊果酸的含量。结果 鉴别项下的阴性对照无干扰,专属性强。熊果酸线性范围0.504～3.024μg,加样回收率为97.90％,RSD=3.07％(n=5)。结论 该方法准确、可靠、专属性强,可用于控制牙周康泰胶囊的质量。

举元颗粒剂质量标准研究

目的：建立举元颗粒剂的质量标准，确保制剂质量。方法：采用薄层色谱法对制剂中的黄芪、甘草进行定性鉴别；采用HPLC法测定制剂中人参皂苷Rg1的含量，色谱条件：LichrospherC18柱（4．6mm×250mm，5um）；流动相乙腈-水（35：65）；柱温25℃，流速1．0ml／min，检测波长203nm。结果：黄芪、甘草的薄层色谱斑点清晰，分离良好；人参皂苷Rg1在0．118～1．416μg范围内线性关系良好，7-0．9997，平均回收率为98．59％。结论：该研究方法专属可靠，可作为该制剂质量控制方法。

伤宁口服液质量标准的研究

目的研究伤宁口服液的质量标准。方法采用TLC法鉴定了伤宁口服液中的黄芪、白术、当归、三七。采用薄层扫描法测定黄芪甲苷的含量,以氯仿-乙酸乙酯-甲醇-水(15∶40∶22∶12,下层)为展开剂,10%硫酸乙醇液显色,测定波长(λS)为530nm,参比波长(λR)为700nm。结果TLC鉴定黄芪、白术、当归、三七专属性强,重现性好;黄芪甲苷点样量在0.6～4.2μg范围内线性关系良好,r=0.9984,平均回收率为97.45%,RSD为3.40%。结论该方法结果可靠,可作为伤宁口服液的质量控制方法。

Chemical Constituents of Hedysarum gmelinii

To study the chemical constituents of Hedysarum gmelinii. Methods Theconstituents were separated and purified by different methods of chromatography, and theirstructures were elucidated by DR, MS and NMR. Results Eight compounds were isolated from Hedysarumgmelinii, including three triteipenoids, two flavonoids and two other compounds. Their structureswere identified as squasapogenol (1), soyasapogenol (2), lupeol (3), 3, 9-dihydroxy coumestan (4),3-hydroxy-9-me-thoxy pterocarpan (5), β-sitosterol (6), palmatic acid (7), and hexadecanoic acid 2,3-dihydroxypropyl ester (8). Conclusion All the compounds have been isolated from this plant forthe first time. Compounds 1 — 4 and 8 were obtained from this genus for the first time. The NMRdata of 1 are reported for the first time.

ISOFLAVONES FROM ASTRAGALUS MEMBRANACEUS

Two new isoflavones (8, 3'-dihydroxy-7,4'-dimethoxyisoflavone, odoratin-7-0-[3-D-glu-copyranoside) and four known isoflavones (formononetin, 7,3'-dihydroxy-8,4'-dimethoxyisoflavone, calycosin, calycosin-7-0-(3-D-glucopyranoside) were isolated from the roots of Astragalus mem-branaceus (Fisch.) Bunge. Their structures were established by spectral analysis.