早期应用大黄中药方治疗百草枯中毒临床研究

目的:探讨早期应用大黄中药方对百草枯中毒患者血清细胞因子及脏器损伤的影响。方法:选择90例百草枯中毒患者,按照就诊顺序分为对照组和观察组各45例。对照组采用常规遵医嘱治疗,观察组在对照组的基础上联合大黄中药方治疗。比较2组患者治疗12 h、24 h、3 d、7 d后血清细胞因子白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)的水平,同时对其治疗后脏器损伤情况、临床疗效及病死率进行比较。结果:观察组治疗24 h、3 d、7 d后,血清细胞因子IL-10、TNF-α、TGF-β1水平优于对照组,差异有统计学意义(P<0.05)。观察组脏器损伤总发生率为33.33%(15/45),显著低于对照组的62.22%(28/45),差异有统计学意义(P<0.05)。观察组总有效率及病死率分别为86.67%(39/45)、13.33%(6/45),优于对照组的75.56%(34/45)、24.44%(11/45),差异有统计学意义(P<0.05)。结论:给予百草枯中毒患者常规遵医嘱治疗的基础上联合早期大黄中药方治疗,减少了脏器损伤的同时降低了患者的病死率,临床效果较好。

黄药水外擦治疗踝关节扭伤47例观察

目的:观察黄药水治疗急性踝关节扭伤的临床疗效。方法:治疗组用黄药水外擦,对照组用正骨水外擦,每天3次,观察1周。结果:治疗组总有效率97.87%,对照组总有效率87.23%,两组比较有显著性差异(P<0.05)。结论:黄药水能加快肿胀消退,减轻疼痛,疗效较好。

《包氏喉证家宝》与包氏家传吹药

目的:探讨秘本《包氏喉证家宝》及其包氏家传吹药。方法:研读《重纂包氏喉证家宝》的原文,归纳、整理、分析所列喉科特效方剂。结果与结论:包氏喉科秘授及其家传吹药的制用对后世医家治疗喉科疾病有重要指导意义。

Effects of galvanic interaction between galena and pyrite on their flotation in the presence of butyl xanthate

The effects of galvanic interaction between galena and pyrite on their flotation and electrochemical characters were studied by electrochemical,adsorption,flotation and FTIR techniques,respectively.Electrochemical tests indicate that galena is electrochemically more active than pyrite and serves as an anode in galvanic combination with pyrite.The galvanic current density from a mixture of galena and pyrite is 4 times as high as the self corrosion current density of galena,which indicates that the corrosion rate of galena is accelerated.Adsorption tests show that the adsorption of butyl xanthate on galena surface is enhanced,and affected by a combination of pyrite-galena mixtures and conditioning time.Compared with individual mineral particles,galvanic interaction reduces the floatability difference between galena and pyrite.The flotation recovery of galena decreases while that of pyrite increases when two minerals are mixed together due to the influence of galvanic interaction on the formation of hydrophilic/hydrophobic product.The FTIR results show that the formation of dixanthogen on pyrite surface is depressed due to the galvanic interaction.

Mechanism of anti-arterial thrombosis of Dahuangzhecong Fang screened by Ti-Al intermetallic compound porous material

The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.

Optimization of extraction and determination of emodin from Polygonum cuspidatum Sieb. et Zucc. products by HPLC-DAD

A uniform experimental design procedure was used to investigate the effects of some operating parameters on the extraction of emodin from Polygonum cuspidatum Sieb. et Zucc. products. Variables tested were volume ratio of material to solvent, size of material, extraction time and temperature and composition of extraction solvent （mixtures of acetone-water）. Each variable was tested at seven levels; 7 experiments were performed in random order. Analyses of the extracts were performed by high-performance liquid chromatography with diode array detection（HPLC-DAD）. Analytical responses were processed by using a forward regression analysis, in order to find polynomial function describing the relationship between variables and responses. For all the analytes the experimental conditions for providing the highest extraction yield inside the experimental domain considered were found. Finally, a simple, rapid and accurate analytical method was developed for the determination of emodin by high performance liquid chromatography. The separation is achieved within 25 rain on an ODS column using methanol and water as gradient mobiles. Emodin can be quantified by using external standard method detecting at 436 nm. Good linearity is obtained with correlation coefficient exceeding 0.9986 and the detection limit and the quantification limit are 1.53 and 3.23 mg/L respectively. This method shows good reproducibility for the quantification of the emodin with intra-day and inter-day relative standard deviation less than 2.3% and 5.6% respectively. Under optimized extraction conditions, the recovery of the standard is 96.5%. The validated method is successfully applied to quantify the emodin in seven Polygonum cuspidatum sieb. Et zucc. products, which provided an idea for the pre-treatment of determination of active compounds in traditional Chinese medicines.

A review on pharmacological effects of Rhizoma Coptidis(Huang lian)

Huang Lian(Rhizoma coptidis, RC), which has effects on clearing damp-heat, quenching fie, and counteracting poison, is a herb frequently used in many traditional formulas. At present, there are many researches on the pharmacological effects of RC. In this review, we summarize the research progress on pharmacological effects of RC from four main aspects, in order to guide clinical application and explore the prospects of the application of RC.

Antibiotic resistance mechanisms of Myroides sp.

Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA （rRNA） gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that ob- taining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infec- tions. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.