黄连子多酚的分离方法研究

通过对黄连子粉的乙醇提取液进行浓缩,将黄连子多酚分为水溶性多酚和水不溶性多酚两部分,采用金属离子沉淀法对水溶性多酚进行分离,得沉淀部分(多酚含量57.14%)和余液部分(多酚含量5.87%)两组分;采用Na HCO3萃取法对水不溶性多酚进行分离,得5%Na HCO3萃取物(多酚含量99.02%)和萃余物(多酚含量99.84%)两组分。通过分离纯化,沉淀组分、5%Na HCO3萃取物和萃余物中多酚的纯度得到显著提高。

黄连子中多酚类物质的提取工艺研究

以黄连子为原料,采用有机溶剂提取法提取黄连子中多酚类物质。首先,通过对比不同溶剂对黄连子多酚的提取效果确定了乙醇作为提取溶剂,在此基础上,采用单因素实验及响应面分析法优化提取工艺。结果表明,黄连子中多酚类物质提取的最佳工艺条件为乙醇浓度64.51%(V/V)、提取时间41.32 min、料液比1∶32.09(W/V)、提取温度46.1℃、提取次数2次。在此条件下,黄连子多酚的提取得率为(4.43±0.02)%。

从六经九分法看热痞证

热痞证在教材中归入太阳病变证,有大黄黄连泻心汤证和附子泻心汤证。通过对症状和方药的分析,认为大黄黄连泻心汤证属阳明病,附子泻心汤证属厥阴病。只有在六经辨证的大框架下,两证才能得到正确认识和充分运用。

Facilitating effects of berberine on rat pancreatic islets through modulating hepatic nuclear factor 4 alpha expression and glucokinase activity

AIM： To observe the effect of berberine on insulin secretion in rat pancreatic islets and to explore its possible molecular mechanism. METHODS： Pdmary rat islets were isolated from male Sprague-Dawley rats by collagenase digestion and treated with different concentrations （1, 3, 10 and 30 μmol/L） of berberine or 1 μmol/L Glibenclamide （GB） for 24 h. Glucose-stimulated insulin secretion （GSIS） assay was conducted and insulin was determined by radioimmunoassay. 3-（4,5-Dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay was performed to evaluate cytotoxicity. The mRNA level of hepatic nuclear factor 4 alpha （HAIF4α） was determined by reverse transcription polymerase chain reaction （RT-PCR）. Indirect immunofluorescence staining and Western blot analysis were employed to detect protein expression of HNF4α in the islets. Glucokinase （GK） activity was measured by spectrophotometric method. RESULTS： Berberine enhanced GSIS rather than basal insulin secretion dose-dependently in rat islets and showed no significant cytotoxicity on islet cells at the concentration of 10 μmol/L. Both mRNA and protein expressions of HNF4α were up-regulated by berberine in a dose-dependent manner, and GK activity was also increased accordingly. However, GB demonstrated no regulatory effects on HNF4α expression or GK activity. CONCLUSION： Berberine can enhance GSIS in rat islets, and probably exerts the insulinotropic effect via a pathway involving HNF4α and GK, which is distinct from sulphonylureas （SUs）.

基于火下阴承、异病同治探讨张仲景鸡子黄类方在2型糖尿病中的应用

2型糖尿病可归属于中医“消渴病”范畴。“火下阴承”源于《黄帝内经》中“君火之下,阴精承之”,而消渴病核心病机及治法与之相契合。张仲景鸡子黄类三方为百合鸡子汤、黄连阿胶汤、排脓散,分散在《伤寒杂病论》不同篇章之中。剖析总结此三方的机理特点,认为符合“火下阴承”的治法特点,契合2型糖尿病之消渴病机。此三方均由鸡子黄不同配伍组成,百合鸡子汤为鸡子黄配伍百合,可治疗2型糖尿病及其合并精神相关疾病;黄连阿胶汤为鸡子黄配伍阿胶、芍药、黄连、黄芩,可治疗2型糖尿病及其合并心肾系统疾病;排脓散为鸡子黄配伍芍药、桔梗、枳实,可用于治疗2型糖尿病并发痈疽。因此,基于“火下阴承”的相关现代研究提出鸡子黄三方在2型糖尿病中的应用,可为临床治疗提供思路。