中成药中黄连成分的测定

采用了三波长—分光光度法测定中成药—黄连素片中黄连成分的含量,有效地消除了随浓度不同使本底漂移,吸收峰不对称给定量分析造成的影响,并校正了基于干扰组分(添加剂,如淀粉等)的吸收光谱具有线性吸收产生的基线倾斜度,实验结果,回收率为97.82％～101.38％。变异系数小于0.24％(n=9),证明方法的精密度与准确度都令人满意。同时,在微型计算机控制下,操作也简单易行。

胡黄连有效成分的提取及药理作用的研究进展

胡黄连为玄参科属植物,具有抗疟、利胆、苦味健脾、治疗肝炎及尿路感染等作用,在临床用于湿热泻痢、小儿疳疾、吐血、黄疸、目赤肿痛、痈肿疮疡、痔疮肿毒及衄血等多种病症。胡黄连主要产于国内的西藏、云南及四川等高原地区,其有效成分复杂,主要为环烯醚萜类、葫芦素糖苷类、酚苷类等。本文就胡黄连的化学成分、提取分离、药理作用等几个方面展开综述,阐述了胡黄连近年来的研究现状。

肝豆灵片中黄连有效成分HPLC定性定量分析

目的优化黄连有效成分的HPLC条件,建立肝豆灵片中黄连相关成分的定性定量分析方法。方法采用HPLC对肝豆灵片中黄连的有效成分进行定性定量分析。流动相为乙腈∶0.05 mol/L磷酸二氢钾溶液=50∶50(每100 mL中加十二烷基硫酸钠0.4 g,再以磷酸调节pH值为4.0),流速为1.0 mL/min,检测波长为345 nm。结果可以鉴别出黄连中表小檗碱、黄连碱、盐酸小檗碱和盐酸巴马汀4个成分,且阴性对照无干扰;盐酸小檗碱对照品进样量在0.058~0.580μg、盐酸巴马汀对照品进样量在0.030~0.300μg范围内与吸收度峰面积值呈良好的线性关系,平均回收率分别为98.18%、99.99%,RSD分别为2.40%、1.70%。结论本研究建立的方法操作简便、准确、重复性好,可用于肝豆灵片的质量控制。

治流感应用双黄连口服液

一、针剂出“问题”，口服液可正常用 不久前，国家通报了青海省大通回族土族自治县患者使用“问题”双黄连注射液后发生不良反应并有人死亡一事，经媒体报道后，不少大众弄不清“是不是所有含双黄连成分的药物都不能用了？比如双黄连口服液还能用吗？”

Baicalin and rutin are major constituents in Shuanghuanglian injection involving anaphylactoid reaction

OBJECTIVE: To identify the constituents in Shuanghuanglian injection(SHLI) that correlate with anaphylactoid reaction.METHODS: Chemical fingerprints of 10 batches SHLI samples were determined by High Performance Liquid Chromatography(HPLC), and further investigated by similarity analysis. Combined with optical microscopy, both anaphylactoid experiments and confirmatory assay were displayed in Rat basophil leukemia cells(RBL-2H3) to obtain the histamine release inducing by SHLI. The content of histamine was tested by Enzyme-Linked Immuno Sorbent As-say method. Partial least squares regression(PLSR)method and HPLC-DAD-ESI-MSntechnology were conducted to analyze constituents in SHLI involving anaphylactoid reaction.RESULTS: The results of spectrum and effect relationships showed that the eight constituents were positively correlated with anaphylactoid reaction.Among which, nearly 90% of them were identified as baicalin and rutin with PLSR and HPLC-DAD-ESI-MSn. This result was in accordance with confirmatory assay on RBL-2H3 cells.CONCLUSION: Baicalin and rutin from SHLI were the main constituents involving anaphylactoid reaction.