为了考察黄金茶提取物防治中波紫外线(UVB)对人表皮角质形成细胞损伤作用,采用不同浓度的黄金茶提取物对HaCaT细胞进行预处理6 h,采用60 m J·cm2的剂量照射细胞,以MTT法检测细胞生存率,吸取细胞上清液检测谷胱甘肽过氧化物酶(GSH-...为了考察黄金茶提取物防治中波紫外线(UVB)对人表皮角质形成细胞损伤作用,采用不同浓度的黄金茶提取物对HaCaT细胞进行预处理6 h,采用60 m J·cm2的剂量照射细胞,以MTT法检测细胞生存率,吸取细胞上清液检测谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和乳酸脱氢酶(LDH)活性以及丙二醛(MDA)含量变化,用流式细胞仪检测细胞凋亡变化。结果表明,UVB辐射对HaCaT细胞造成严重损伤,黄金茶提取物可提高UVB照射后HaCaT细胞的存活率,提高GSH-Px、SOD活性,降低LDH活性和MDA含量,同时黄金茶提取物能够降低UVB所引起的细胞凋亡率升高(P<0.01),还可以减少UVB诱导HaCaT细胞损伤或凋亡,具有一定的光保护功能,其作用机制可能与清除氧自由基和增强抗氧化能力有关。展开更多
In this paper,we have studied the adsorption properties of gold by α-alkyl substituted pyridine resin( BPR) in alkaline solution.The single and dual component kinetic mass transfer process of Au(I),Ag(I) and other me...In this paper,we have studied the adsorption properties of gold by α-alkyl substituted pyridine resin( BPR) in alkaline solution.The single and dual component kinetic mass transfer process of Au(I),Ag(I) and other metal cyanide ions were also investigated.The results obtained show that the mass transfer rates of the cyanide ions in the resin phase are closely related to the molar ratio and the configuration of metal cyanide ions. The elution kinetics of Au(I) and other cyanide ions by NH4SCN has been investigated systematically.The intraparticle diffusion coefficient and activation energy of Au(I),Ag(I),Ni(I) Fe(Ⅲ） cyanide anions were determined in CI,NO3,OH-type BPR resin at different temperatures.It is found from the experimental results that the mechanism for extracting Au(I),Ag(I) and other metal cyanide anions by BPR resin belongs to ion exchange process.The difference of activation entropy of some metal cyanide anions(ΔS-ΔS^-) was evaluated.展开更多
The technology that waste activated carbon after extracting gold is regenerated with steam under microwave heating was studied. The influence of the activation temperature, activation duration and steam flow rate on i...The technology that waste activated carbon after extracting gold is regenerated with steam under microwave heating was studied. The influence of the activation temperature, activation duration and steam flow rate on iodine adsorption value and regeneration yield of activated carbon was investigated. The response surface methodology （RSM） technique was utilized to optimize the process conditions. The optimum conditions for the preparation of activated carbon are identified to be activation temperature of 831 ℃, activation duration of 40 min and steam flow rate of 2.67 mL/min. The optimum conditions result in an activated carbon with an iodine number of 1048 mg/g and a yield of 40%, and the BET surface area evaluated using nitrogen adsorption isotherm is 1493 m2/g, with total pore volume of 1.242 cm3/g. And the pore structure of activated carbon regenerated is mainly composed of micropores and a small amount of mesopores.展开更多
The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chr...The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chromatography using Avicel as the adsorbent. After that, 50% of methanol in water was used as the eluting solvent and continued to further purify by Avicel preparative layer chromatogram 2 times using 80% of methanol in water as developing solvent. MIC of pure compound was done by two fold dilution method. Muller Hinton agar was selected as the media. The new compound was found as combretin, a steroidal alkaloid, and showed antibacterial activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 better than Staphylococcus aureus ATCC 25923.展开更多
With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogona...With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogonal experiment, this experiment optimizes process condition of flavonoid in Trollius chinensis through water extraction and ethanol extraction. The result shows that flavonoid extraction rate of Trollius chinensis through ethanol extraction method is obviously higher than water extraction, and the optimal extraction process condition is： ethanol concentration is 75%, extraction time is 90min, extraction times are 3, and fluid material ratio is 20：1.展开更多
In the present paper, the ethyl acetate extract from the fruiting bodies of Tirmania pinoyi （Maire） was obtained by Soxhlet extraction. Six fractions were separated from this extract using two chromatographic method...In the present paper, the ethyl acetate extract from the fruiting bodies of Tirmania pinoyi （Maire） was obtained by Soxhlet extraction. Six fractions were separated from this extract using two chromatographic methods. All these fractions were submitted to antimicrobial activity against four clinically important bacteria Staphylococcus aureus ATCC6538, Enterococcusfaecalis ATCC6538 Pseudomonas aeruginosa ATCC14028, Escherichia coli ATCC25922 and one pathogenic fungus Candida albicans ATCCI0231. The in vitro antimicrobial activity was performed by agar disc diffusion method. The fractions with the greatest antimicrobial activity were fractions 02 and 06 which inhibited growth of both Gram negative and Gram positive bacteria and had significant antifungal activity against Candida albicans. The present study validates the folk use of the boiled truffle water-extract and indicates that it could be effective potential candidates for the development of new strategies to treat bacterial or fungal infections.展开更多
This study aimed to determine the antimicrobial activity of beehive extract and developed topical antimicrobial gel. The beehives were extracted with three different solvents： distilled water, 50% ethanol and 95% eth...This study aimed to determine the antimicrobial activity of beehive extract and developed topical antimicrobial gel. The beehives were extracted with three different solvents： distilled water, 50% ethanol and 95% ethanol, then each filtrate was evaporated by vacuum rotary evaporator to obtain crude extracts： water extract （W）, water-ethanol extract （WE） and ethanol extract （E）. These extracts were tested for their antimicrobial activity against five bacterial strains： Escherichia coli （ATCC 25922）, Psuedomonas aeuruginosa （ATCC 27853）, Staphylococcus aureus （ATCC 25923）, Methicillin Resistance Staphylococcus aureus （MRSA） and β-hemolytic Streptococcus group A （GAS）, then they were evaluated for their MIC and MBC values. The results revealed that the yield of the W, WE and E extracts amounted to 16.25%, 14.42% and 6.67%, respectively. Whereas, all of the samples were found to be effective against P. aeuruginosa, S. aureus, MRSA and GAS, but they were ineffective against E. coli. The W extract exhibited the highest antimicrobial activity with MIC values of 31.25-62.50 mg/mL and MBC values of 62.50 mg/mL. Then the gel bases using different gelling agents such as carbopol, hydroxyethylcellulose （HEC） and hydroxypropyl methylcellulose （HPMC） were formulated and evaluated for their physical properties： appearance, texture, pH, spread ability and stability. The formula which contained HEC was selected to incorporate with the W extract due to its smooth texture, good spreadability on the skin and good stability. The W gel exhibited a promising antimicrobial activity in both before and after stability test and also expressed no skin irritation.展开更多
Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower f...Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower flavonoid extract(SAFE) exhibits the neuroprotective effect. In this study, the safflower flavonoid extract drop pills(SAFE-DPs) for anti-PD were prepared by the heating and melting method using SAFE and matrix PEG6000. The performances of the pills were evaluated with powder X-ray diffraction(PXRD), differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy(FT-IR), scanning electron microscopy(SEM) and dissolution testing. The analysis results demonstrated an amorphous state for SAFE dispersion in the matrix PEG6000 without any chemical reaction. The SAFE-DPs demonstrated acceptable chemical and physical stability irrespective of the manufacturing process and the storage period. Dissolution testing in three dissolution media(pH 1.0, pH 6.8 and pH 7.5) indicated that SAFE-DPs had excellent dissolution property. The transport of Kaempferol-3-rutinoside(K3 R) on the Caco-2 monolayer and the absorption of K3 R in situ intestinal perfusion revealed that the principal component of SAFE had a good transport and absorption capacity. Therefore, the drop pills had better release and absorption in the gastrointestinal tract, corresponding with the pharmacological and pharmacodynamic results for PD in vivo.展开更多
文摘为了考察黄金茶提取物防治中波紫外线(UVB)对人表皮角质形成细胞损伤作用,采用不同浓度的黄金茶提取物对HaCaT细胞进行预处理6 h,采用60 m J·cm2的剂量照射细胞,以MTT法检测细胞生存率,吸取细胞上清液检测谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和乳酸脱氢酶(LDH)活性以及丙二醛(MDA)含量变化,用流式细胞仪检测细胞凋亡变化。结果表明,UVB辐射对HaCaT细胞造成严重损伤,黄金茶提取物可提高UVB照射后HaCaT细胞的存活率,提高GSH-Px、SOD活性,降低LDH活性和MDA含量,同时黄金茶提取物能够降低UVB所引起的细胞凋亡率升高(P<0.01),还可以减少UVB诱导HaCaT细胞损伤或凋亡,具有一定的光保护功能,其作用机制可能与清除氧自由基和增强抗氧化能力有关。
文摘In this paper,we have studied the adsorption properties of gold by α-alkyl substituted pyridine resin( BPR) in alkaline solution.The single and dual component kinetic mass transfer process of Au(I),Ag(I) and other metal cyanide ions were also investigated.The results obtained show that the mass transfer rates of the cyanide ions in the resin phase are closely related to the molar ratio and the configuration of metal cyanide ions. The elution kinetics of Au(I) and other cyanide ions by NH4SCN has been investigated systematically.The intraparticle diffusion coefficient and activation energy of Au(I),Ag(I),Ni(I) Fe(Ⅲ） cyanide anions were determined in CI,NO3,OH-type BPR resin at different temperatures.It is found from the experimental results that the mechanism for extracting Au(I),Ag(I) and other metal cyanide anions by BPR resin belongs to ion exchange process.The difference of activation entropy of some metal cyanide anions(ΔS-ΔS^-) was evaluated.
基金Project(2013AA064003)supported by the National High Technology Research and Development Program of ChinaProject(2012HB008)supported by Young and Middle-aged Academic Technology Leader Backup Talent Cultivation Program in Yunnan Province,China
文摘The technology that waste activated carbon after extracting gold is regenerated with steam under microwave heating was studied. The influence of the activation temperature, activation duration and steam flow rate on iodine adsorption value and regeneration yield of activated carbon was investigated. The response surface methodology （RSM） technique was utilized to optimize the process conditions. The optimum conditions for the preparation of activated carbon are identified to be activation temperature of 831 ℃, activation duration of 40 min and steam flow rate of 2.67 mL/min. The optimum conditions result in an activated carbon with an iodine number of 1048 mg/g and a yield of 40%, and the BET surface area evaluated using nitrogen adsorption isotherm is 1493 m2/g, with total pore volume of 1.242 cm3/g. And the pore structure of activated carbon regenerated is mainly composed of micropores and a small amount of mesopores.
文摘The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chromatography using Avicel as the adsorbent. After that, 50% of methanol in water was used as the eluting solvent and continued to further purify by Avicel preparative layer chromatogram 2 times using 80% of methanol in water as developing solvent. MIC of pure compound was done by two fold dilution method. Muller Hinton agar was selected as the media. The new compound was found as combretin, a steroidal alkaloid, and showed antibacterial activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 better than Staphylococcus aureus ATCC 25923.
文摘With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogonal experiment, this experiment optimizes process condition of flavonoid in Trollius chinensis through water extraction and ethanol extraction. The result shows that flavonoid extraction rate of Trollius chinensis through ethanol extraction method is obviously higher than water extraction, and the optimal extraction process condition is： ethanol concentration is 75%, extraction time is 90min, extraction times are 3, and fluid material ratio is 20：1.
文摘In the present paper, the ethyl acetate extract from the fruiting bodies of Tirmania pinoyi （Maire） was obtained by Soxhlet extraction. Six fractions were separated from this extract using two chromatographic methods. All these fractions were submitted to antimicrobial activity against four clinically important bacteria Staphylococcus aureus ATCC6538, Enterococcusfaecalis ATCC6538 Pseudomonas aeruginosa ATCC14028, Escherichia coli ATCC25922 and one pathogenic fungus Candida albicans ATCCI0231. The in vitro antimicrobial activity was performed by agar disc diffusion method. The fractions with the greatest antimicrobial activity were fractions 02 and 06 which inhibited growth of both Gram negative and Gram positive bacteria and had significant antifungal activity against Candida albicans. The present study validates the folk use of the boiled truffle water-extract and indicates that it could be effective potential candidates for the development of new strategies to treat bacterial or fungal infections.
文摘This study aimed to determine the antimicrobial activity of beehive extract and developed topical antimicrobial gel. The beehives were extracted with three different solvents： distilled water, 50% ethanol and 95% ethanol, then each filtrate was evaporated by vacuum rotary evaporator to obtain crude extracts： water extract （W）, water-ethanol extract （WE） and ethanol extract （E）. These extracts were tested for their antimicrobial activity against five bacterial strains： Escherichia coli （ATCC 25922）, Psuedomonas aeuruginosa （ATCC 27853）, Staphylococcus aureus （ATCC 25923）, Methicillin Resistance Staphylococcus aureus （MRSA） and β-hemolytic Streptococcus group A （GAS）, then they were evaluated for their MIC and MBC values. The results revealed that the yield of the W, WE and E extracts amounted to 16.25%, 14.42% and 6.67%, respectively. Whereas, all of the samples were found to be effective against P. aeuruginosa, S. aureus, MRSA and GAS, but they were ineffective against E. coli. The W extract exhibited the highest antimicrobial activity with MIC values of 31.25-62.50 mg/mL and MBC values of 62.50 mg/mL. Then the gel bases using different gelling agents such as carbopol, hydroxyethylcellulose （HEC） and hydroxypropyl methylcellulose （HPMC） were formulated and evaluated for their physical properties： appearance, texture, pH, spread ability and stability. The formula which contained HEC was selected to incorporate with the W extract due to its smooth texture, good spreadability on the skin and good stability. The W gel exhibited a promising antimicrobial activity in both before and after stability test and also expressed no skin irritation.
基金Science and Technology Major Projects:Significant New-Drugs Creation(Grant No.2012ZX09103201-042)
文摘Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower flavonoid extract(SAFE) exhibits the neuroprotective effect. In this study, the safflower flavonoid extract drop pills(SAFE-DPs) for anti-PD were prepared by the heating and melting method using SAFE and matrix PEG6000. The performances of the pills were evaluated with powder X-ray diffraction(PXRD), differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy(FT-IR), scanning electron microscopy(SEM) and dissolution testing. The analysis results demonstrated an amorphous state for SAFE dispersion in the matrix PEG6000 without any chemical reaction. The SAFE-DPs demonstrated acceptable chemical and physical stability irrespective of the manufacturing process and the storage period. Dissolution testing in three dissolution media(pH 1.0, pH 6.8 and pH 7.5) indicated that SAFE-DPs had excellent dissolution property. The transport of Kaempferol-3-rutinoside(K3 R) on the Caco-2 monolayer and the absorption of K3 R in situ intestinal perfusion revealed that the principal component of SAFE had a good transport and absorption capacity. Therefore, the drop pills had better release and absorption in the gastrointestinal tract, corresponding with the pharmacological and pharmacodynamic results for PD in vivo.
