In this paper,Sulfobacillus thermosulfidooxidans ST was selected for use in bioleaching of pyrite and chalcopyrite.The adsorption experiments revealed that more cells were adsorbed on the surface of pyrite than on the...In this paper,Sulfobacillus thermosulfidooxidans ST was selected for use in bioleaching of pyrite and chalcopyrite.The adsorption experiments revealed that more cells were adsorbed on the surface of pyrite than on the surface of chalcopyrite.The role of extracellular DNA(eDNA)in the bioleaching process was investigated by depletion of eDNA using DNase I.The number of cells attached on the chalcopyrite and pyrite surfaces decreased on a large scale,and the lag phase of cell growth increased,causing the leaching percentages of pyrite and chalcopyrite to decrease by approximately 11.6%and 20.5%,respectively.The formation and distribution of eDNA secreted during bioleaching was assessed by a fluorescent dye-based method and visualized by confocal laser scanning microscopy(CLSM).The content of eDNA increased with bioleaching time.Furthermore,ST showed a stronger capacity to produce eDNA on the surface of pyrite than on the surface of chalcopyrite.These results showed that the removal of eDNA has a more significant effect on the bioleaching of chalcopyrite than on pyrite.展开更多
基金Projects(31470230,51320105006,51604308)supported by the National Natural Science Foundation of ChinaProject(2017RS3003)supported by the Youth Talent Foundation of Hunan Province of China+1 种基金Project(2018JJ2486)supported by the Natural Science Foundation of Hunan Province of ChinaProject(2018WK2012)supported by the Key Research and Development Projects in Hunan Province,China。
文摘In this paper,Sulfobacillus thermosulfidooxidans ST was selected for use in bioleaching of pyrite and chalcopyrite.The adsorption experiments revealed that more cells were adsorbed on the surface of pyrite than on the surface of chalcopyrite.The role of extracellular DNA(eDNA)in the bioleaching process was investigated by depletion of eDNA using DNase I.The number of cells attached on the chalcopyrite and pyrite surfaces decreased on a large scale,and the lag phase of cell growth increased,causing the leaching percentages of pyrite and chalcopyrite to decrease by approximately 11.6%and 20.5%,respectively.The formation and distribution of eDNA secreted during bioleaching was assessed by a fluorescent dye-based method and visualized by confocal laser scanning microscopy(CLSM).The content of eDNA increased with bioleaching time.Furthermore,ST showed a stronger capacity to produce eDNA on the surface of pyrite than on the surface of chalcopyrite.These results showed that the removal of eDNA has a more significant effect on the bioleaching of chalcopyrite than on pyrite.
