苍黄间落红一地(外一首)

蓦然发觉我激昂的词汇是秦筑长城时掉落的一块青砖。

Identification of Molecular Markers for the Thinopyrum intermedium Chromosome 2Ai-2 with Resistance to Barley Yellow Dwarf Virus

The wheat_ Thinopyrum intermedium addition lines Z1,Z2 contain a pair of Th. intermedium chromosomes 2Ai_2 carrying the gene with resistance to barley yellow dwarf virus (BYDV). Genomic in situ hybridization (GISH) was used to analyze the chromosome constitution of Z1,Z2 by using genomic DNA probes from Th. intermedium and Pseudoroegneria strigosa . The results showed that the chromosome constitution of either Z1 or Z2 composes of 42 wheat chromosomes and two Th. intermedium chromosomes (2Ai_2). The 2Ai_2 chromosome is St_E intercalary translocation, in which the E genomic chromosome segment translocated into the middle region of the long arm of chromosome belonging to St genome. With the genomic DNA probe of Ps. strigosa , the GISH pattern specific to the 2Ai_2 chromosome may be used as a molecular cytogenetic marker. A detailed RFLP analysis on Z1, Z2 and their parents was carried out by using 12 probes on the wheat group 2 chromosomes. Twenty RFLP markers specific to the 2Ai_2 chromosome were identified. Two RAPD markers of OPR16 -350 and OPH09 -1580 , specific to the 2Ai_2 chromosome, were identified from 280 RAPD primers. These molecular markers could be used to assisted_select translocation lines with small segment of the 2Ai_2 chromosome and provide tools to localize the BYDV resistance.

化学振荡

我们从一些资料出发,通过一些研究得到以下几种体系,可供各学校根据不同情况选用。

A Fast and Indirect Fluorescent Antibody Assay for the Vibrio in Large Yellow Croaker Pseudosciaena crocea (Richardson)

A fast and indirect fluorescent antibody assay for the Vibrio alginolyticus and V. parahaemolyticus infecting the large yellow croaker has been developed. The specific antisera for the two strains of vibrio were prepared with New Zealand rabbit and the antiserum and cross-reactive efficacy was tested by coagulation in tube. It showed that the goat anti-rabbit IgG had been labeled by fluorescence isothiocyanate (FITC). The results showed that positive reactions were 100% for the large yellow croaker Pseudosciaena crocea with typical symptom of vibrio infection, while the positive reaction to the pathogen in healthy yellow croakers reached 40%, but seemed negative for aquaculture water. The results demonstrated that this fast and indirect fluorescent antibody assay can be used not only to test the vibrio pathogen in diseased yellow croaker but also in infected animals with no symptom.

Mechanism of anti-arterial thrombosis of Dahuangzhecong Fang screened by Ti-Al intermetallic compound porous material

The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.

Denitrification and Nitrate Reduction to Ammoniumin Taihu Lake and Yellow Sea Inter-Tidal MarineSediments

Denitrification and nitrate reduction to ammonium in Taihu Lake and Yellow Sea inter-tidal marinesediments were studied. The sediment samples were made into slurry containing 150 g dry matter per liter.Various amounts of glucose and 5 mmol L-1 of potassium nitrate were added in order to achieve differentratios of glucose-C to nitrate-N. Acetylene inhibition technique was applied to measure denitrification in theslumes. All samples were incubated anaerobically under argon atmosphere. Data showed that Taihu Lakesediment produced more N2O than marine sediment. Denitrification potential was higher in Taihu Lakesediment than in marne one. Glucose added increased denitrification activity but not the denitrification po-tential of the sediments. Dissimilatory nitrate reduction to ammonium seemed to occur in marine sediment,but not in freshwater one. When the marine sediment was treated with 25 mmol L-1 glucose, its denitrification poteatial, as indicated by maximum N2O production by acetylene blockage, was lower than that treatedwith no or 2.5 mmol L-l glucose. Acetylene was suspected to have inhibitory effect on dissimilatory nitratereduction to ammonium.

Long-term variability of the sharp thermocline in the Yellow and East China Seas

Based on observed temperature data since the 1950s, long-term variability of the summer sharp thermocline in the Yellow Sea Cold Water Mass （YSCWM） and East China Sea Cold Eddy （ECSCE） areas is examined. Relationships between the thermocline and atmospheric and oceanic forcing were investigated using multiyear wind, Kuroshio discharge and air temperature data. Results show that： 1） In the YSCWM area, thermocline strength shows about 4-year and 16-year period oscillations. There is high correlation between summer thermocline strength and local atmospheric temperature in summer and the previous winter; 2） In the ECSCE area, interannual oscillation of thermocline strength with about a 4-year period （stronger in El Nifio years） is strongly correlated with that of local wind stress. A transition from weak to strong thermocline during the mid 1970s is consistent with a 1976/1977 climate shift and Kuroshio volume transport; 3） Long-term changes of the thermocline in both regions are mainly determined by deep layer water, especially on the decadal timescale. However, surface water can modify the thermocline on an interannual timescale in the YSCWM area.

Breeding of an F_1 Hybrid Cultivar Hangjiao Huangshuai by Space Mutation Breeding Technique

Hangjiao Huangshuai is a processing type yellow pepper F1 hybrid cultivar bred with the inbred line 032-1-2-1-1-H-H bred through space treatment as the female parent and 082-1-1-H-H free of space treatment as the male parent. The fruit is in the shape of long finger with few wrinkles and good gloss, and tastes very spicy .The fruit has a vertical diameter of 17.5 cm, a transverse diameter of 1.6 cm, a fresh thickness of 0,20 cm and a single fruit weight of 15.6 g. It is yellowishgreen in the early maturation stage and yellow in the late maturation stage. The cultivar exhibited high resistance to pepper blight, viral diseases and powdery mildew, and showed an average yield of 56 493,0 kg/hm^2. It could be generalized in Gansu, Ningxia, Liaoning and Zhejiang.

DNA barcode assessment of Ceramiales(Rhodophyta) in the intertidal zone of the northwestern Yellow Sea

A total of 142 specimens of Ceramiales （Rhodophyta） were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species, 14 genera, and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers, namely, partial large subunit rRNA gene （LSU）, universal plastid amplicon （UPA）, and partial mitochondrial cytochrome c oxidase subunit I gene （COl）. No intraspecific divergence was found in our collection for these markers, except for a 1-3 bp divergence in the COI of Ceramium kondoi, Syrnphyocladia latiuscula, and Neosiphoniajaponica. Because short DNA markers were used, the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in GenBank or BOLD （Barcode of Life Data） Systems. Three specimens were presumed as Heterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification, which was the first record in the investigated area, implying that it might he a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers, Heterosiphonia japonica converged with Dasya spp. and was distant from the other Heterosiphonia spp., implying that H.japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species, but the COI had a higher ability to discriminate between species.

Analysis of Nonstationary Wind Fluctuations Using the Hilbert-Huang Transform

Climatological patterns in wind fluctuations on time scales of 1–10 h are analyzed at a meteorological mast at the Yangmeishan wind farm, Yunnan Province,China, using a 2-yr time series of 10-min wind speed observations. For analyzing the spectral properties of nonstationary wind fluctuations in mountain terrain, the Hilbert-Huang transform(HHT) is applied to investigate climatological patterns between wind variability and several variables including time of year, time of day, wind direction, and pressure tendency. Compared with that for offshore sites, the wind variability at Yangmeishan wind farm has a more distinct diurnal cycle, but the seasonal discrepancies and the differences according to directions are not distinct, and the synoptic influences on wind variability are weaker. There is enhanced variability in spring and winter compared with summer and autumn. For flow from the main direction sector, the maximum wind variability is observed in spring. And the severe wind fluctuations are more common when the pressure tendency is rising.

ROLE OF INTERVENING OPPORTUNITY IN TOURIST DESTINATION DEVELOPMENT

Intervening opportunity is an important factor in spatial interaction, and has very important impacts on tourist destination development in a region. There are few literatures on this topic in tourist researches, and especially in China fewer tourist researchers pay attention to intervening opportunity theory. This article, taking Lin’an as a case study, aims to introduce intervening opportunity theory in tourist destination development by the comprehensive ana-lysis of some data including literatures, Internet information, longitudinal data and some tourist surveys. Both qualitative and quantitative methods are all used in this research. Firstly, the authors expatiate upon the definition of intervening opportunity, by reviewing the literatures in detail, take Lin’an of Zhejiang Province as a case study, and then analyze some necessary factors of intervening opportunity, such as regional tourist demands and supplies, tourist attractions, spatial distance and transportation, tourist images and costs, regional competition and cooperation in tourist destinations. Finally the impacts of intervening opportunity on Lin’an tourist development are analyzed in detail. The results show that intervening opportunity do not occur everywhere, and there must be some critical factors, and intervening opportunity surely plays important roles in tourist destination development. The tourist development in Lin’an is attributed to intervening opportunity of the spatial location between Hangzhou and Huangshan that are two famous tourist cities in China, and the occurrence of intervening opportunities in Lin’an is the integration of some external and internal factors.

Interannual variability of the southern Yellow Sea Cold Water Mass

Temperature data collected in the sections of 34°N, 35°N and 36°N in August from 1975 through2003 were analyzed using Empirical Orthogonal Function (EOF) to investigate interannual variability of the southern Yellow Sea Cold Water Mass (YSCWM). The first mode (EOF1) reveals variations of basin-wide thermocline depth, which is mainly caused by surface heating. The second mode (EOF2) presents fluctuations of vertical circulation, resulting mainly from interannual variability of cold front intensity. In addition, it is found that the upward extent of upwelling in the cold front is basically determined by wind stress curl and the zonal position of the warm water center in the southern Yellow Sea is correlated with spatial difference of net heat flux.

Spatial distribution of dimethylsulfide and dimethyl-sulfoniopropionate in the Yellow Sea and Bohai Sea during summer

The distributions of dimethylsulfide （DMS） and its precursor dimethylsulfoniopropionate （DMSP） in surface water of the Yellow Sea and the Bohai Sea were studied during June 2011. The mean concentrations and ranges of DMS, dissolved DMSP （DMSPd）, and particulate DMSP （DMSPp） in surface waters were 6.85 （1.60-12.36）, 7.25 （2.28-19.05） and 61.87 （6.28-224.01） nmol/L, respectively. There were strong correlations between DMSPp and chlorophyll a in the Bohai Sea and the North Yellow Sea, respectively, and concentrations of DMS and DMSP were high, with a relatively high proportion of dinoflagellates, in the region of the South Yellow Sea Cold Water Mass. Results show that phytoplankton biomass and species composition were important factors that controlled the distribution of DMS and DMSP. Complex environmental factors, including nutrients, transparency, and terrestrial runoff, might also influence the variability in DMS and DMSP. Biological production and consumption rates of DMS in the Bohai Sea were higher than those in the Yellow Sea. DMS production rates were closely correlated with DMSPd concentrations. DMS and DMSP exhibited obvious diel variations, with high concentrations occurring in the late afternoon （16：00-19：00） and low concentrations occurring during the night, implying that the intensity of solar radiation had a significant influence on these variations. Size distributions of chlorophyll a and DMSPp were also investigated and large nanoplankton （5-20 μm）, mainly diatoms, contributed significantly to chlorophyll a and DMSPp at most stations. The average sea-to-air flux of DMS in the study area was estimated to be 11.07 μmol/（m^2·d） during the summer.

Nitrogen Biological Cycle Characteristics of Seepweed(Suaeda salsa) Wetland in Intertidal Zone of Huanghe(Yellow) River Estuary

From April 2008 to November 2009, the nitrogen （N） cycle of plant-soil system in seepweed （Suaeda salsa） wetland in the intertidal zone of the Huanghe （Yellow） River estuary was studied. Results showed that soil N had sig- nificant seasonal fluctuations and vertical distribution, and the net N mineralization rates in topsoil were significantly different in growing season （p 〈 0.01）. The N/P ratio （9.87 ±1.23） of S. salsa was less than 14, indicating that plant growth was limited by N. The N accumulated in S. salsa litter at all times during decomposition, which was ascribed to the N immobilization by microbes from the environment. Soil organic N was the main N stock of plant-soil system, accounting for 97.35% of the total N stock. The N absorption and utilization coefficients of S. salsa were very low （0.0145 and 0.3844, respectively）, while the N cycle coefficient was high （0.7108）. The results of the N turnovers among compartments of S. salsa wetland showed that the N uptake amount of aboveground part and root were 7.764 g/m2and 4.332 g/m2, respectively. The N translocation amounts from aboveground part to root and from root to soil were 3.881 g/m2 and 0.626 g/m2, respectively. The N translocation amount from aboveground living body to litter was 3.883 g/m2, the annual N return amount from litter to soil was more than 0.125（-） g/m2 （minus represented immobili- zation）, and the net N mineralization amount in topsoil （0-15 cm） in growing season was 1.190 g/m2. The assessment of N biological cycle status orS. salsa wetland indicated that N was a very important limiting factor and the ecosystem was situated in unstable and vulnerable status. The S. salsa was seemingly well adapted to the low-nutrient status and vulnerable habitat, and the N quantitative relationships determined in the compartment model might provide scientific base for us to reveal the special adaptive strategy orS. salsa to the vulnerable habitat in the following studies.

Meso-scale spatial distribution of large tintinnids in early summer in southern Yellow Sea

The spatial distribution of some large tintinnid species (nominally>76 μm) was investigated according to samples collected by vertical towing in cruises to the southern Yellow Sea in summer 2000-2002 and 2004. Eight species were identified: Codonellopsis mobilis, Leprotintinnus netritus, Tintinnopsis karajacensis, T. japonica, T. kiaochowensis, T. butschlii, T. radix, and Parafavella sp. With maximum abundance of 158.2 ind/L in June 2004, C. mobilis was the dominant species, lasting from May to July 2004. Tintinnid communities were patchy and distributed mainly in shallow waters along the shore.

The Labors of the Months and the Zodiac Signs in the Cathedral of Otranto： An Iconographical Interpretation of the Symbols of Labor and Time

In 1163, Archbishop Gionata, Abbot of the Monastery of San Nicola of Casole in Puglia （Apulia）, commissioned Pantaleone, a learned monk and artist from the monastery, to decorate the pavement of the cathedral of Otranto with mosaics. This Italian Romanesque cathedral was dedicated to Santa Maria Annunziata or Saint Mary of The Annunciation. In the interior, the floor design in the shape of a tree contains an elaborate biblical program. The encyclopedic narrative of the mosaic symbolically unveils the meaning of the Tree of Life. The ieonography of the pavement＇s program combines biblical and pagan narratives evolving from a natural realm into a heavenly realm. The natural realm begins at the church＇s entrance with the symbolism of the earth-the roots of a tree-expanding through the nave and transept via the tree＇s trunk and its branches, culminating in the apse, at the end of the church, with the foliage oftbe top of the tree. This natural realm meets with the heavenly realm in two ways： at the crossing of the church or at the sacrificial altar, and at the apse of the church. Before reaching the altar or holy area, between the natural realm and the heavenly realm, the zodiac signs with the labors of the months are depicted.

Different Treatment Methods on Seed Germination Seedlings

[Objective] This study was conducted to investigate the effects of different gibberellic acid and hydrogen peroxide concentrations and different soaking time on the germination potential and germination rate of Scutellaria baicalensis, so as to improve the breeding efficiency of S. baicalensis. [Method] Dry seeds were divided into 3 groups, each containing 50 grains, which were weighed and put in a culture dish and soaked with distilled water covering the surface of seeds at room temperature. The seed weight was measured by gravimetric method once every 30 min from 30 rain after soaking, until the weight of the seeds no longer changed. [Result] With the increase of the concentration of hydrogen peroxide, the germination rate of S. baicalensis was improved gradually, and reached 32.0% under the concentration of 1.0% at the soaking time of 24 h; and with the increase of the concentration of gibberellic acid, the germination rate of S. baicalensis was improved gradually, and reached 64.0% under the concentration of 600 mg/L at the soaking time of 24 h. [Conclusion] The constant temperature at 25 ℃ in the incubator could also improve the seed germination rate; and the germination rate achieved by treating the seeds with gibbereUic acid was remarkably higher than that achieved by treating the seeds with hydrogen peroxide.

Tupu methods of spatial-temporal pattern on land use change:a case study in the Yellow River Delta

Comprehensive study on land-use change of spatial pattern and temporal process is the key component in LUCC study nowadays. Based on the theories and methods of Geo-information Tupu (Carto-methodology in Geo-information, CMGI), integration of spatial pattern and temporal processes of land-use change in the Yellow River Delta (YRD) are studied in the paper, which is supported by ERDAS and ARC/INFO software. The main contents include: (1) concept models of Tupu by spatial-temporal integration on land-use change, whose Tupu unit is synthesized by "Spatial·Attribute·Process" features and composed of relatively homogeneous geographical unit and temporal unit; (2) data sources and handling process, where four stages of spatial features in 1956, 1984, 1991, and 1996 are acquired; (3) integration of series of temporal-spatial Tupu, reconstruction series of "Arising" Tupu, spatial-temporal Process Tupu and the spatial temporal Pattern Tupu on land-use change by remap tables; (4) Pattern Tupu analysis on land-use change in YRD during 1956-1996; and (5) spatial difference of the Pattern Tupu analysis by dynamic Tupu units. The various landform units and seven sub-deltas generated by the Yellow River since 1855 are different. The Tupu analysis on land-use in the paper is a promising try on the comprehensive research of "spatial pattern of dynamic process" and "temporal process of spatial pattern" in LUCC research. The Tupu methodology would be a powerful and efficient tool on integrated studies of spatial pattern and temporal process in Geo-science.

Tempo-spatial Variation of Nutrient and Chlorophyll-a Concentrations From Summer to Winter in the Zhangzi Island Area (Northern Yellow Sea)

Nutrient and Chlorophyll-a （Chl-a） concentrations were investigated monthly along three transects extending from a mariculturc area to open waters around the Zhangzi Island area from July to December 2009. The objective of this study is to illus- trate food availability to the bottom-sowed scallop Patinopecten yessoensis under the influences of the Yellow Sea Cold Water Mass （YSCWM）, freshwater input and feedbacks of cultivated scallops. Significant thermal stratification was present in open waters from July to October, and salinity decreased in July and August in surface layers in the mariculture area. Nutrient concentrations increased with depth in both areas in summer, but were similar through water column in November and December. On average, nutrient in- creased from summer to autumn in all components except ammonia. Nutrient concentrations lower than the minimum thresholds for phytoplankton growth were present only in upper layers in summer, but stoichiometric nitrogen limitation existed in the entire inves- tigation period. Column-averaged Chl-a concentration was lower in open waters than in mariculture area in all months. It increased significantly in mariculturc area in August and October, and was less variable in open waters. Our results show that nutrients limita- tion to phytoplankton growth is present mainly in upper layer in association with stratification caused by YSCWM in summer. Freshwater input and upwelling of nutrients accumulated in YSCWM can stimulate phytoplankton production in mariculture area. Farming activities may change stoichiometric nutrient ratios but have less influence on Chl-a concentration.

Spatial Variability of Soil Organic Carbon in a Watershed on the Loess Plateau

Soil organic carbon (SOC) has great impacts on global warming, land degradation and food security. Classic statistical and geostatistical methods were used to characterize and compare the spatial heterogeneity of SOC and related factors, such as topography, soil type and land use, in the Liudaogou watershed on the Loess Plateau of North China. SOC concentrations followed a log-normal distribution with an arithmetic and geometric means of 23.4 and 21.3 g kg-1, respectively, were moderately variable (CV = 75.9%), and demonstrated a moderate spatial dependence according to the nugget ratio (34.7%). The experimental variogram of SOC was best-fitted by a spherical model, after the spatial outliers had been detected and subsequently eliminated. Lower SOC concentrations were associated with higher elevations. Warp soils and farmland had the highest SOC concentrations, while aeolian sand soil and shrublands had the lowest SOC values. The geostatistical characteristics of SOC for the different soil and land use types were different. These patterns were closely related to the spatial structure of topography, and soil and land use types.