在评价黄鱼胶营养价值的基础上。以水解度(Degree of hydrolysis,DH)为评价指标,对酶解时间、酶解温度、酶解pH值、复合酶比例和酶添加量分别进行单因素试验,进而以响应面优化得出其最佳的酶解工艺条件。结果表明:黄鱼胶基本成分蛋白质...在评价黄鱼胶营养价值的基础上。以水解度(Degree of hydrolysis,DH)为评价指标,对酶解时间、酶解温度、酶解pH值、复合酶比例和酶添加量分别进行单因素试验,进而以响应面优化得出其最佳的酶解工艺条件。结果表明:黄鱼胶基本成分蛋白质、脂肪、多糖、灰分的含量分别为82.2%、2.00%、0.22%、0.83%。黄鱼胶中水解氨基酸含量高达81.7%,其中甘氨酸占总水解氨基酸的24.72%。制备鱼胶原蛋白肽的最佳酶解工艺为酶解时间4.67 h、酶解温度56.73℃、酶解pH值8.02、中性蛋白酶和碱性蛋白酶的复合比例1:3、酶添加量1.5%,此时鱼胶的水解度可达39.94%。综上,黄鱼胶的胶原蛋白含量丰富,研究可为黄鱼胶的复合酶解工艺提供参考。展开更多
T his study compared and evaluated the efficiency of two otolith shape descriptors(i.e., the elliptic Fourier transform(EFT) and discrete wavelet transform(DWT)) and morphometrics for stock discrimination. To accompli...T his study compared and evaluated the efficiency of two otolith shape descriptors(i.e., the elliptic Fourier transform(EFT) and discrete wavelet transform(DWT)) and morphometrics for stock discrimination. To accomplish this, sample fish from three stocks of yellow croaker L arimichthys polyactis along the Chinese coast(LDB stock from the Liaodong Bay of the Bohai Sea, JZB stock from the Jiaozhou Bay of the Yellow Sea and CJE stock from the Changjiang River estuary of the East China Sea) were used for otolith morphology analyses. The results showed that morphometrics produced an overall classification success rate of 70.8% in contrast with success rates of 80.0% or 82.0% obtained using EFT or DWT, respectively. This suggests that the two shape descriptors comparably discriminated among the stocks and performed more efficiently than morphometrics. During data adjustment and acquisition, some size variables were excluded from the subsequent discriminant analysis for stock discrimination because they were statistically "inef fective," which could reduce the efficiency of morphometrics and lead to relatively low overall classification success. Both EFT and DWT retain the contour coefficients and thus provide a detailed description of otolith shape, which could improve discriminatory efficiency compared with morphometrics.展开更多
文摘在评价黄鱼胶营养价值的基础上。以水解度(Degree of hydrolysis,DH)为评价指标,对酶解时间、酶解温度、酶解pH值、复合酶比例和酶添加量分别进行单因素试验,进而以响应面优化得出其最佳的酶解工艺条件。结果表明:黄鱼胶基本成分蛋白质、脂肪、多糖、灰分的含量分别为82.2%、2.00%、0.22%、0.83%。黄鱼胶中水解氨基酸含量高达81.7%,其中甘氨酸占总水解氨基酸的24.72%。制备鱼胶原蛋白肽的最佳酶解工艺为酶解时间4.67 h、酶解温度56.73℃、酶解pH值8.02、中性蛋白酶和碱性蛋白酶的复合比例1:3、酶添加量1.5%,此时鱼胶的水解度可达39.94%。综上,黄鱼胶的胶原蛋白含量丰富,研究可为黄鱼胶的复合酶解工艺提供参考。
基金Supported by the National Key Basic Research Program of China(973 Program)(No.2015CB453302)the NSFC-Shandong Joint Fund for Marine Science Research Centre(No.U1606404)the Aoshan Science and Technology Innovation Project(No.2015ASKJ02-04)
文摘T his study compared and evaluated the efficiency of two otolith shape descriptors(i.e., the elliptic Fourier transform(EFT) and discrete wavelet transform(DWT)) and morphometrics for stock discrimination. To accomplish this, sample fish from three stocks of yellow croaker L arimichthys polyactis along the Chinese coast(LDB stock from the Liaodong Bay of the Bohai Sea, JZB stock from the Jiaozhou Bay of the Yellow Sea and CJE stock from the Changjiang River estuary of the East China Sea) were used for otolith morphology analyses. The results showed that morphometrics produced an overall classification success rate of 70.8% in contrast with success rates of 80.0% or 82.0% obtained using EFT or DWT, respectively. This suggests that the two shape descriptors comparably discriminated among the stocks and performed more efficiently than morphometrics. During data adjustment and acquisition, some size variables were excluded from the subsequent discriminant analysis for stock discrimination because they were statistically "inef fective," which could reduce the efficiency of morphometrics and lead to relatively low overall classification success. Both EFT and DWT retain the contour coefficients and thus provide a detailed description of otolith shape, which could improve discriminatory efficiency compared with morphometrics.
