目的探讨芪黄煎剂对大鼠胃切除后小肠黏膜上皮内淋巴细胞(intraepithelial lymphocytes,IELs)和固有层淋巴细胞(lamina propria lymphocytes,LPLs)的影响。方法将60只大鼠随机分为假手术组、模型组、芪黄煎剂组,每组20只。假手术组大鼠...目的探讨芪黄煎剂对大鼠胃切除后小肠黏膜上皮内淋巴细胞(intraepithelial lymphocytes,IELs)和固有层淋巴细胞(lamina propria lymphocytes,LPLs)的影响。方法将60只大鼠随机分为假手术组、模型组、芪黄煎剂组,每组20只。假手术组大鼠仅给予腹部正中切开后缝合,不行胃切除,不给予肠内营养和芪黄煎剂;模型组大鼠行胃切除手术后给予肠内营养制剂能全素;芪黄煎剂组大鼠行胃切除手术后给予肠内营养制剂能全素和芪黄煎剂,疗程1周。疗程结束后,分离出大鼠小肠黏膜IELs和LPLs,采用流式细胞仪分别检测IELs和LPLs的αβT细胞抗原受体-白细胞分化抗原3阳性(αβT cell antigen receptor-cluster ofdifferentiation 3positive,αβTCR-CD3+)T细胞、白细胞分化抗原4阳性(cluster of differentiation 4positive,CD4+)、白细胞分化抗原8阳性(cluster of differentiation 8positive,CD8+)T淋巴细胞。结果①与假手术组比较,模型组IELs中αβTCR-CD3+、CD8+T细胞比例显著降低(P<0.05,或P<0.01);与模型组比较,芪黄煎剂组IEL CD3+、CD8+T细胞比例显著上升(P<0.05,或P<0.01)。②与假手术组比较,模型组LPLs中αβTCR-CD3+、CD4+、CD8+T细胞比例显著降低(P<0.01);与模型组比较,芪黄煎剂组αβTCR-CD3+、CD4+T细胞比例显著上升(P<0.05)。结论芪黄煎剂能促进大鼠胃切除术后上皮内和固有层中T淋巴细胞的分化、成熟和增殖,有利于胃切除手术应激后肠道免疫屏障功能的调节和恢复。展开更多
Aim: Iron deficiency anaemia is a frequent finding seen in coeliac disease, which can be diagnosed alone or with other findings. In this study, our aim was to determine the prevalence of coeliac disease in children wi...Aim: Iron deficiency anaemia is a frequent finding seen in coeliac disease, which can be diagnosed alone or with other findings. In this study, our aim was to determine the prevalence of coeliac disease in children with iron deficiency anaemia without significant gastrointestinal symptoms. Methods: There were 135 children with iron deficiency anaemia in the patient group (group 1), and 223 healthy children without iron deficiency anaemia in the control group (group 2) in this study. Antiendomysial antibody (EMA) IgA test was given to both groups. Antiendomysial antibody-positive patients underwent small intestine biopsy. Results: The mean age was 7.2 ±4.6 (2-16) y in the patient group (group 1) and 8.2 ±3.8 (2-16) y in the control group (group 2), and no significant difference between the two groups was detected. In terms of gender, there was a significant difference between groups 1 and 2 (M/F: 74/61 and 98/125, respectively) (p < 0.05). EMA was positive in six cases in group 1 (4.4%), and villous atrophy and/or inflammation in the lamina propria with increased intraepithelial lymphocytes was seen on small intestine biopsy in these patients. In the control group, EMA was negative in all children. In detailed histories of patients with coeliac disease diagnosis, recurrent iron deficiency anaemia/pica was found in four patients (66.7%) and occasionally foul-smelling or watery stool attacks were seen in four patients (66.7%). Three of these six patients (50%) had short stature. Conclusion: The prevalence of coeliac disease was high in patients with iron deficiency anaemia; therefore, gastrointestinal findings should be further examined for coeliac disease, and the possibility of coeliac disease should be investigated in patients with recurrent iron deficiency anaemia and short stature.展开更多
文摘Aim: Iron deficiency anaemia is a frequent finding seen in coeliac disease, which can be diagnosed alone or with other findings. In this study, our aim was to determine the prevalence of coeliac disease in children with iron deficiency anaemia without significant gastrointestinal symptoms. Methods: There were 135 children with iron deficiency anaemia in the patient group (group 1), and 223 healthy children without iron deficiency anaemia in the control group (group 2) in this study. Antiendomysial antibody (EMA) IgA test was given to both groups. Antiendomysial antibody-positive patients underwent small intestine biopsy. Results: The mean age was 7.2 ±4.6 (2-16) y in the patient group (group 1) and 8.2 ±3.8 (2-16) y in the control group (group 2), and no significant difference between the two groups was detected. In terms of gender, there was a significant difference between groups 1 and 2 (M/F: 74/61 and 98/125, respectively) (p < 0.05). EMA was positive in six cases in group 1 (4.4%), and villous atrophy and/or inflammation in the lamina propria with increased intraepithelial lymphocytes was seen on small intestine biopsy in these patients. In the control group, EMA was negative in all children. In detailed histories of patients with coeliac disease diagnosis, recurrent iron deficiency anaemia/pica was found in four patients (66.7%) and occasionally foul-smelling or watery stool attacks were seen in four patients (66.7%). Three of these six patients (50%) had short stature. Conclusion: The prevalence of coeliac disease was high in patients with iron deficiency anaemia; therefore, gastrointestinal findings should be further examined for coeliac disease, and the possibility of coeliac disease should be investigated in patients with recurrent iron deficiency anaemia and short stature.
