In recent years,remarkable progress has been made in the research of injectable hydrogel for internal tissue healing.However,the therapeutic outcome is usually limited when the hydrogel is used for the treatment of ga...In recent years,remarkable progress has been made in the research of injectable hydrogel for internal tissue healing.However,the therapeutic outcome is usually limited when the hydrogel is used for the treatment of gastric perforation due to the high acidic gastric juice and violent deformation of the gastric wall.Regarding these challenges,we proposed an ionic nano-reservoir(INR)-based dual-network hydrogel,which has excellent adhesion and mechanical properties,and can be easily applied to the perforation site to block the perforation while promoting tissue repairing.The results showed that the first network made of polyacrylamide had cross-linked on the stomach tissue within 5 s under blue light,and enhanced the adhesion performance through mechanical interlock.The nano-hydroxyapatite acted as ionic INR,which can gradually release Ca^(2+) under acid environments to form the second network with sodium alginate and inhibit the swelling of hydrogel in gastric juice.Meanwhile,the adhesion was further enhanced through amide covalent bonds at the hydrogel-tissue interface with the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide(EDC/NHS).The dual network hydrogels obtained by the INR strategy could be employed as a potential therapeutic option for gastric perforation and other similar biomedical prolems.展开更多
Scavenging reactive oxygen species(ROS)by antioxidants has been demonstrated as the most effective strategy for preventing percutaneous coronary intervention(PCI)-related complications.However,topical and long-term de...Scavenging reactive oxygen species(ROS)by antioxidants has been demonstrated as the most effective strategy for preventing percutaneous coronary intervention(PCI)-related complications.However,topical and long-term delivery of ROS antioxidants to a specific vascular tissue is proven to be a great challenge.Herein,an ROS scavenger of 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl(TEMPOL)is incorporated into electrospun nanofibers with a tunable loading amount to achieve its topical applicability and long-term antiradical capability.Biological functions of such TEMPOL-loaded electrospun membranes are evaluated by cell proliferation,ROS-scavenging capability,monocyte adhesion,cell migration,inflammatory molecule secretion and mRNA expression in vitro.After optimizing the loading amount of TEMPOL,such an electrospun membrane presents a superior ROS-scavenging and anti-inflammation performance for both endothelial cells and macrophages.The expression of endothelial prothrombogenic molecules and the migration of vascular smooth muscle cells(VSMCs)are also effectively inhibited.Thus,it is bravely predicted that the topical use of such a TEMPOL-loaded electrospun system will be a promising pathway for the anti-restenosis therapy,especially when used as a novel coating on stent for long-term and topical delivery of antioxidant drugs.展开更多
基金supported by the National Natural Science Foundation of China(81971701,51832001,and 81901873)the Natural Science Foundation of Jiangsu Province(BK20201352)the Program of Jiangsu Specially-Appointed Professor。
文摘In recent years,remarkable progress has been made in the research of injectable hydrogel for internal tissue healing.However,the therapeutic outcome is usually limited when the hydrogel is used for the treatment of gastric perforation due to the high acidic gastric juice and violent deformation of the gastric wall.Regarding these challenges,we proposed an ionic nano-reservoir(INR)-based dual-network hydrogel,which has excellent adhesion and mechanical properties,and can be easily applied to the perforation site to block the perforation while promoting tissue repairing.The results showed that the first network made of polyacrylamide had cross-linked on the stomach tissue within 5 s under blue light,and enhanced the adhesion performance through mechanical interlock.The nano-hydroxyapatite acted as ionic INR,which can gradually release Ca^(2+) under acid environments to form the second network with sodium alginate and inhibit the swelling of hydrogel in gastric juice.Meanwhile,the adhesion was further enhanced through amide covalent bonds at the hydrogel-tissue interface with the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide(EDC/NHS).The dual network hydrogels obtained by the INR strategy could be employed as a potential therapeutic option for gastric perforation and other similar biomedical prolems.
基金the National Key Research and Development Program of China(2016YFA0201702 and 2016YFA0201700)the National Natural Science Foundation of China(51733002,81670319 and 81521001)+2 种基金the Fundamental Research Funds for the Central Universities(19D110618)the Overseas Expertise Introduction Project for Discipline Innovation(111-2-04)the Chinese Academy of Medical Sciences’Innovation Fund for Medical Sciences(CAMS,2019-I2M-5-060)。
文摘Scavenging reactive oxygen species(ROS)by antioxidants has been demonstrated as the most effective strategy for preventing percutaneous coronary intervention(PCI)-related complications.However,topical and long-term delivery of ROS antioxidants to a specific vascular tissue is proven to be a great challenge.Herein,an ROS scavenger of 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl(TEMPOL)is incorporated into electrospun nanofibers with a tunable loading amount to achieve its topical applicability and long-term antiradical capability.Biological functions of such TEMPOL-loaded electrospun membranes are evaluated by cell proliferation,ROS-scavenging capability,monocyte adhesion,cell migration,inflammatory molecule secretion and mRNA expression in vitro.After optimizing the loading amount of TEMPOL,such an electrospun membrane presents a superior ROS-scavenging and anti-inflammation performance for both endothelial cells and macrophages.The expression of endothelial prothrombogenic molecules and the migration of vascular smooth muscle cells(VSMCs)are also effectively inhibited.Thus,it is bravely predicted that the topical use of such a TEMPOL-loaded electrospun system will be a promising pathway for the anti-restenosis therapy,especially when used as a novel coating on stent for long-term and topical delivery of antioxidant drugs.
