为开发不同风味的蓝靛果酵素,对黑曲霉纤维素酶在蓝靛果酵素中的作用进行研究,在蓝靛果中接种0.5%的乳酸片球菌,添加或不添加黑曲霉纤维素酶进行发酵并用气质联用仪和高效液相色谱仪对产品进行分析。结果表明,添加黑曲霉纤维素酶后蓝靛...为开发不同风味的蓝靛果酵素,对黑曲霉纤维素酶在蓝靛果酵素中的作用进行研究,在蓝靛果中接种0.5%的乳酸片球菌,添加或不添加黑曲霉纤维素酶进行发酵并用气质联用仪和高效液相色谱仪对产品进行分析。结果表明,添加黑曲霉纤维素酶后蓝靛果酵素(fermented blue honeysuckle berry cellulase,FBHB-C)的挥发性化合物共有44种,不添加黑曲霉纤维素酶的蓝靛果酵素(fermented blue honeysuckle berry,FBHB)有42种,两个样品的醇类化合物含量分别占各总挥发性化合物含量的75.95%和24.77%、酯类化合物占总挥发性化合物含量的4.38%和53.00%、酮类化合物占3.17%和11.38%、醛类化合物占14.06%和6.33%、其他化合物占2.44%和4.51%,FBHB-C主要挥发性化合物为顺式-4-己烯-1-醇、正己醇、1-辛醇、苯甲醛、仲辛酮、苯甲酸乙酯。在两种蓝靛果酵素中检出有机酸7种,柠檬酸含量最多,FBHB-C中草酸、酒石酸、苹果酸、富马酸、马来酸的含量比FBHB中相应有机酸分别提高130.38%、88.03%、196.55%、1509.52%、156.27%,其中草酸、酒石酸、柠檬酸呈香贡献最大。展开更多
[Objective] The research aimed to breed the high-yield production strain of cellulase.[Method] Aspergillus niger was used as the starting strain,and a high-yield production strain of cellulase was selected after UV mu...[Objective] The research aimed to breed the high-yield production strain of cellulase.[Method] Aspergillus niger was used as the starting strain,and a high-yield production strain of cellulase was selected after UV mutation treatment.[Result] Under the suitable condition,the strain 2（15） with the highest CMC production capacity was selected,which nearly increased 50% than that of the starting strain.[Conclusion] The research provided the foundation for its appliation in the feed production in the future.展开更多
文摘为开发不同风味的蓝靛果酵素,对黑曲霉纤维素酶在蓝靛果酵素中的作用进行研究,在蓝靛果中接种0.5%的乳酸片球菌,添加或不添加黑曲霉纤维素酶进行发酵并用气质联用仪和高效液相色谱仪对产品进行分析。结果表明,添加黑曲霉纤维素酶后蓝靛果酵素(fermented blue honeysuckle berry cellulase,FBHB-C)的挥发性化合物共有44种,不添加黑曲霉纤维素酶的蓝靛果酵素(fermented blue honeysuckle berry,FBHB)有42种,两个样品的醇类化合物含量分别占各总挥发性化合物含量的75.95%和24.77%、酯类化合物占总挥发性化合物含量的4.38%和53.00%、酮类化合物占3.17%和11.38%、醛类化合物占14.06%和6.33%、其他化合物占2.44%和4.51%,FBHB-C主要挥发性化合物为顺式-4-己烯-1-醇、正己醇、1-辛醇、苯甲醛、仲辛酮、苯甲酸乙酯。在两种蓝靛果酵素中检出有机酸7种,柠檬酸含量最多,FBHB-C中草酸、酒石酸、苹果酸、富马酸、马来酸的含量比FBHB中相应有机酸分别提高130.38%、88.03%、196.55%、1509.52%、156.27%,其中草酸、酒石酸、柠檬酸呈香贡献最大。
基金Supported by Natural Foundation of Education Department of HenanProvince (2010B530001)Research Project of Department of Sci-ence and Technology in Henan Province (102102310391)~~
文摘[Objective] The research aimed to breed the high-yield production strain of cellulase.[Method] Aspergillus niger was used as the starting strain,and a high-yield production strain of cellulase was selected after UV mutation treatment.[Result] Under the suitable condition,the strain 2（15） with the highest CMC production capacity was selected,which nearly increased 50% than that of the starting strain.[Conclusion] The research provided the foundation for its appliation in the feed production in the future.
