两株外生菌根真菌对盐渍土壤中黑松幼苗生长的影响

为了研究外生菌根真菌对宿主植物黑松幼苗耐盐性的影响,采用盆栽实验,将成功侵染菌种土生空团菌(Cg)、紫晶蜡蘑(La)的黑松幼苗移栽于盐渍土壤中,培育3个月后,测定相关生理指标。结果表明:1)与非菌根化幼苗相比,接种菌根真菌Cg和La显著提高了针叶叶绿素a(由0.78 mg/g FW增加到1.27 mg/g FW和1.47 mg/g FW)和叶绿素b(由0.28 mg/g FW增加到0.40 mg/g FW和0.43 mg/g FW)的含量,增加了宿主体内脯氨酸含量(地上部:La> Cg> NM;地下部:La> Cg> NM),增强了K^+的吸收、转运,有效地维持(Cg菌种)或是降低(La菌种)了宿主地上部分Na^+/K^+的比值;显著(P <0.05)降低了宿主幼苗地下部的Na^+/K^+比值(由0.70降低到0.47和0.64)。2)外生菌根真菌有利于松树幼苗的生长,提高了其耐盐性,但因菌种而异(La> Cg)。深入研究不同菌种对不同宿主植物耐盐性的影响,将会成为后续菌根技术研究的一个重要方向,为沿海滩涂盐碱地的植被恢复提供理论依据及技术支持。

不同氮源对黑松幼苗根-土界面无机磷形态转化及有效性的影响

用根垫-冰冻切片法研究不同氮源对石灰性潮土中黑松幼苗根-土界面无机磷形态转化及有效性的影响。结果表明:铵态氮(NH4+-N)处理后幼苗根-土界面pH值较对照处理明显降低,而硝态氮(NO3--N)处理后幼苗根-土界面pH值较对照处理升高,不同氮源引起的幼苗根-土界面pH值变动的幅度取决于氮源的质量分数。铵态氮处理明显降低了幼苗根-土界面Ca2-P,Fe-P和Al-P质量分数,100,200和400mg·kg-1的铵态氮处理后,距根表0～1mm处Ca2-P较土体亏缺率分别为37.1%,45.9%和57.7%,Fe-P较土体亏缺率分别为23.4%,29.1%和38.2%,Al-P较土体亏缺率分别为25.1%,28.0%和33.2%;硝态氮处理增加了幼苗根-土界面Ca2-P,Fe-P和Al-P质量分数,但不显著。铵态氮与硝态氮处理后幼苗根-土界面Ca8-P的亏缺程度较对照分别加大或降低,但不明显。铵态氮与硝态氮处理后幼苗根-土界面Ca10-P和O-P质量分数较对照处理变化很小。铵态氮引起的黑松幼苗根-土界面pH值的降低,促进了幼苗根-土界面处无机磷的形态转化,提高了根-土界面无机磷的生物有效性,显著增加了根系对磷的吸收。

Electrical wave transmission in healthy black pine seedlings

Non-injurious local stimuli, such as a cold shock, and injurious stimuli, such as local burning, punctures or chemicals, were applied to study electrical wave transmission in black pine (Pinus thunbergii) seedlings. The results showed that non-injurious stimuli evoked the action potential (AP) transmission and injurious stimulation induced both AP transmission and the more complex variation (VP) transmission in the seedlings. The causes of these phenomena were discussed. Key words Black pine - Pinus thunbergii - Action potential (AP) - Variation potential (VP) - Electrical wave transmission CLC number S791.256 Document code A Foundation item: This paper was supported by the National Nature Science Foundation of China (grant No. 39670613).Biography: GUO Jian (1971-), male, lecturer in Haikou Bureau of Forestry. Hainan, P. R. China.Responsible editor: Song Funan