Karyotype analysis of Lycium ruthenicum Murr. was carried out in this study. The results showed that the chromosome number was 2n=2x=24; the arm index was 48; the ratio of the longest chromosome to the shortest one wa...Karyotype analysis of Lycium ruthenicum Murr. was carried out in this study. The results showed that the chromosome number was 2n=2x=24; the arm index was 48; the ratio of the longest chromosome to the shortest one was 1.31; the proportions of chromosomes with arm ratio higher than 2 was 0.08; the asymmetry index was 57.02; the karyotype type was 2A; and the karyotype formula was 2n=-24=20m+4sm.展开更多
Mitotic metaphase chromosomes of 34 species of Drosophila melanogaster species group were examined. Certain new karyotypes were described for the first time, and their evolutionary and interspecific genetic relationsh...Mitotic metaphase chromosomes of 34 species of Drosophila melanogaster species group were examined. Certain new karyotypes were described for the first time, and their evolutionary and interspecific genetic relationships among 8 subgroups of D. melanogaster species group were analyzed systematically. The results were as follows. The basic karyotype of elegans subgroup was type A. The karyotypes of eugracilis subgroup, melanogaster subgroup, and ficusphila subgroup were all type C. The karyotypes of takahashii subgroup and suzukii subgroup were both type C and type D. The montium subgroup had six kinds of karyotypes types B, C, C', D, D', and E. The ananassae subgroup had three kinds of karyotypes: types F, G, and H. Thus, the melanogaster species group was classified into five pedigrees based on the diversity of these karyotypes: 1) elegans; 2) eugracilis-melanogasterficusphila; 3) takkahashii-suzukii; 4) montium; 5) ananassae. The above-mentioned results in karyotypic evolution were consistent with those of DNA sequence analysis reported by Yang except for the elegans subgroup and this subgroup was considered as the ancestral subgroup. Karyotype analysis of the same drosophila from different isofemale lines indicated that the same Drosophila from different places showed karyotypic variation which might be due to different geographical environment and evolutionary degree or interaction between the two factors.展开更多
The chromosome karyotypes of Chorthippus (Megaulacobothrus) aethalinus (Zubovsky) and Ch. (M.) chinensis Tarbinsky were compared. Results show that the chromosome numbers of the two species fit 2n (♂) = 17 = ...The chromosome karyotypes of Chorthippus (Megaulacobothrus) aethalinus (Zubovsky) and Ch. (M.) chinensis Tarbinsky were compared. Results show that the chromosome numbers of the two species fit 2n (♂) = 17 = 16 + XO, in which three pairs of autosomal and sex chromosomes were terminal chromosomes, and the other five pairs of autosomes were metacentric chromosomes. These are diagnosis characters for Chorthippus. However, these two species can be distinguished by the different chromosome formula (CF) and the relative length (RL). The CF in Ch. (M.) aethalinus is K (2n, ♂) = 6m + 11t = 6L + 6M + 4S + XO, whereas that of Ch. (M.) chinensis is K (2n, ♂) = 6m + 11t = 6L + 8M + 2S + XO. In addition, we also found that the relative lengths of sex chromosomes in the two species were different from each other. The sex-chromosome of Ch. (M.) aethalinus is located at the fifth position and its relative length is 8.33% whereas that of Ch. (M.) chinensis is at the eighth position and its relative length is 5.53%. These results show that significantly different genetic features exist for the two species of Chorthippus.展开更多
[Objective] This study was conducted optimize the conditions for the ex- traction of melanin from walnut husk. [Method] Melanin was isolated from walnut husk through alkali extraction and acid precipitation, and the f...[Objective] This study was conducted optimize the conditions for the ex- traction of melanin from walnut husk. [Method] Melanin was isolated from walnut husk through alkali extraction and acid precipitation, and the factors that may influ- ence the extraction efficiency: temperature, duration, NaOH concentration and solid- liquid ratio were set at different levels. [Result] The optimal conditions for the ex- traction of melanin from walhut husk were temperature of 80 ℃, extraction duration of 120 min, NaOH concentration of 70 mol/L, and solid-liquid ratio of 1:20. [Conclu- sion] The optimal conditions for the extraction of melanin from walnut husk deter- mined in the present study will provide references for the development and utiliza- tion of natural melanin.展开更多
A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 col...A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of~29 kD. It is a rRNA N-glycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC_(50) of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M_(21) with a IC_(50) of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.展开更多
The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema turris Ehr. var. latum Fan et Wang. var. nov. and Gomphonema ac...The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema turris Ehr. var. latum Fan et Wang. var. nov. and Gomphonema acuminatum var. obtusum Fan et Bao, var. nov., that were distinguished by frustule morphology under LM. The samples were collected from freshwater of Heilongjiang Province, China.展开更多
Based on the coexistence of the Blandford-Znajek and magnetic coupling processes in black hole (BH) accretion disc, a multi-parameter model for jet powers and radio loudnees of active galactic nuclei (AGNs) is stu...Based on the coexistence of the Blandford-Znajek and magnetic coupling processes in black hole (BH) accretion disc, a multi-parameter model for jet powers and radio loudnees of active galactic nuclei (AGNs) is studied. It turns out that radio-loudnees of AGNs couM be governed by five parameters: (i) the BH spin, (ii) a power-law index of the variation of the magnetic field on the disc; (iii) a parameter determining the position of the inner edge of the disc, (iv) the ratio of the pressure of the magnetic field on the horizon to the ram pressure of the innermost parts of an accretion flow, and (v) the ratio of the angular velocity of the open field lines to that of the horizon. The observed dichotomy between radio-loud and radio-quiet AGNs is well interpreted by the effects of the above parameters. Furthermore, we discuss the derivative of radio loudness of AGNs with respect to each parameter separately. In addition, the effect of the screw instability on radio loudness of AGNs is discussed.展开更多
基金Supported by National Natural Science Foundation of China(31100401)Tianjin Science and Technology Plan Project(13ZLZLZF05700)+1 种基金Fund for Special Science and Technology Correspondents of Tianjin City(15JCTPJC59500)College Students'Innovative Entrepreneurial Training Program of Tianjin City(201610061102)~~
文摘Karyotype analysis of Lycium ruthenicum Murr. was carried out in this study. The results showed that the chromosome number was 2n=2x=24; the arm index was 48; the ratio of the longest chromosome to the shortest one was 1.31; the proportions of chromosomes with arm ratio higher than 2 was 0.08; the asymmetry index was 57.02; the karyotype type was 2A; and the karyotype formula was 2n=-24=20m+4sm.
基金This work was supported by National Natural Sciences Foundation of China (No. 39930100) International Collaborative Project of Hubei Provincial Department of Education (No. G200610001).
文摘Mitotic metaphase chromosomes of 34 species of Drosophila melanogaster species group were examined. Certain new karyotypes were described for the first time, and their evolutionary and interspecific genetic relationships among 8 subgroups of D. melanogaster species group were analyzed systematically. The results were as follows. The basic karyotype of elegans subgroup was type A. The karyotypes of eugracilis subgroup, melanogaster subgroup, and ficusphila subgroup were all type C. The karyotypes of takahashii subgroup and suzukii subgroup were both type C and type D. The montium subgroup had six kinds of karyotypes types B, C, C', D, D', and E. The ananassae subgroup had three kinds of karyotypes: types F, G, and H. Thus, the melanogaster species group was classified into five pedigrees based on the diversity of these karyotypes: 1) elegans; 2) eugracilis-melanogasterficusphila; 3) takkahashii-suzukii; 4) montium; 5) ananassae. The above-mentioned results in karyotypic evolution were consistent with those of DNA sequence analysis reported by Yang except for the elegans subgroup and this subgroup was considered as the ancestral subgroup. Karyotype analysis of the same drosophila from different isofemale lines indicated that the same Drosophila from different places showed karyotypic variation which might be due to different geographical environment and evolutionary degree or interaction between the two factors.
基金supported by the Special fund for basic scientific research project in the central scientific research institutes (Institute of Grassland Research of CAAS)(1610332012007)
文摘The chromosome karyotypes of Chorthippus (Megaulacobothrus) aethalinus (Zubovsky) and Ch. (M.) chinensis Tarbinsky were compared. Results show that the chromosome numbers of the two species fit 2n (♂) = 17 = 16 + XO, in which three pairs of autosomal and sex chromosomes were terminal chromosomes, and the other five pairs of autosomes were metacentric chromosomes. These are diagnosis characters for Chorthippus. However, these two species can be distinguished by the different chromosome formula (CF) and the relative length (RL). The CF in Ch. (M.) aethalinus is K (2n, ♂) = 6m + 11t = 6L + 6M + 4S + XO, whereas that of Ch. (M.) chinensis is K (2n, ♂) = 6m + 11t = 6L + 8M + 2S + XO. In addition, we also found that the relative lengths of sex chromosomes in the two species were different from each other. The sex-chromosome of Ch. (M.) aethalinus is located at the fifth position and its relative length is 8.33% whereas that of Ch. (M.) chinensis is at the eighth position and its relative length is 5.53%. These results show that significantly different genetic features exist for the two species of Chorthippus.
基金Supported by National Innovative Entrepreneurship Training Program for College Students(201510379029)the Fund of Suzhou Collaborative Innovation Center for Regional Development(2015SZXTZXKFZD01)+1 种基金the Fund of Scientific Research Platform of Suzhou University(2015ykf02)Higher Education Quality Engineering Project of Department of Education of Anhui Province(2016ckjh197)~~
文摘[Objective] This study was conducted optimize the conditions for the ex- traction of melanin from walnut husk. [Method] Melanin was isolated from walnut husk through alkali extraction and acid precipitation, and the factors that may influ- ence the extraction efficiency: temperature, duration, NaOH concentration and solid- liquid ratio were set at different levels. [Result] The optimal conditions for the ex- traction of melanin from walhut husk were temperature of 80 ℃, extraction duration of 120 min, NaOH concentration of 70 mol/L, and solid-liquid ratio of 1:20. [Conclu- sion] The optimal conditions for the extraction of melanin from walnut husk deter- mined in the present study will provide references for the development and utiliza- tion of natural melanin.
文摘A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of~29 kD. It is a rRNA N-glycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC_(50) of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M_(21) with a IC_(50) of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.
文摘The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema turris Ehr. var. latum Fan et Wang. var. nov. and Gomphonema acuminatum var. obtusum Fan et Bao, var. nov., that were distinguished by frustule morphology under LM. The samples were collected from freshwater of Heilongjiang Province, China.
基金The project supported by National Natural Science Foundation of China under Grant Nos. 10173004, 10373006, and 10121503
文摘Based on the coexistence of the Blandford-Znajek and magnetic coupling processes in black hole (BH) accretion disc, a multi-parameter model for jet powers and radio loudnees of active galactic nuclei (AGNs) is studied. It turns out that radio-loudnees of AGNs couM be governed by five parameters: (i) the BH spin, (ii) a power-law index of the variation of the magnetic field on the disc; (iii) a parameter determining the position of the inner edge of the disc, (iv) the ratio of the pressure of the magnetic field on the horizon to the ram pressure of the innermost parts of an accretion flow, and (v) the ratio of the angular velocity of the open field lines to that of the horizon. The observed dichotomy between radio-loud and radio-quiet AGNs is well interpreted by the effects of the above parameters. Furthermore, we discuss the derivative of radio loudness of AGNs with respect to each parameter separately. In addition, the effect of the screw instability on radio loudness of AGNs is discussed.