A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles （PD-MNPs） was prepared and characterized. The widely used Aspetyillus niger lipase （ANL） was immobilized on the PD-MNPs （ANL...A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles （PD-MNPs） was prepared and characterized. The widely used Aspetyillus niger lipase （ANL） was immobilized on the PD-MNPs （ANL@PD-MNPs） with a protein loading of 138 mg/g and an activity recovery of 83.6% under optimized conditions. For the immobilization, the pH and immobilization time were investigated. The pH and thermal and storage stability of the ANL@PD-MNPs significant- ly surpassed those of free ANL. The ANL@PD-MNPs had better solvent tolerance than free ANL. The secondary structure of free ANL and ANL@PD-MNPs was analyzed by infrared spectroscopy, A kinetic study demonstrated that the ANL@PD-MNPs had enhanced enzyme-substrate affinity and high catalytic efficiency. The ANL@PD-MNPs was applied as a biocatalyst for the regioselective acylation of dihydromyricetin （DMY） in DMSO and gave a conversion of 79.3%, which was higher than that of previous reports. The ANL@PD-MNPs retained over 55% of its initial activity after 10 cycles of reuse. The ANL@PD-MNPs were readily separated from the reaction system by a magnet. The PD-MNPs is an excellent support for ANL and the resulting ANL@PD-MNPs displayed good potential for the efficient synthesis of dihydromyricetin-3-acetate by enzymatic regioselective acylation.展开更多
Settling particulate matter(SPM) is widely used to describe the sedimentary environment.We have investigated here the SPM collected with a time-series sediment trap in the Heini Bay,Weihai,Shandong Peninsula,China,dur...Settling particulate matter(SPM) is widely used to describe the sedimentary environment.We have investigated here the SPM collected with a time-series sediment trap in the Heini Bay,Weihai,Shandong Peninsula,China,during the super typhoon Muifa(August 2011,maximum wind speed 55 m/s).Meteorological and hydrological parameters were measured for 18 days.By analyzing the particle flux,grain size,and loss-on-ignition(organic matter content) of SPM we found dramatic changes in these parameters,induced by typhoon Muifa for about 6 days.With the arrival of typhoon,the daily SPM fluxes increased sharply to the maximum at 76.4 g/(m^2·d) on the first day and this increase is about 9 times of the normal value,and after 6 days the SPM decreased to the normal value gradually.Other parameters,such as grain size of SPM and organic matter also experienced similar changes.Using these SPM parameters,we divided the settling progress by cluster analysis into three phases:strong(for 3 days),weak(for 3 days),and zero typhoon impact.展开更多
基金supported by the National Natural Science Foundation of China(21336002,21222606,21376096)the Key Program of Guangdong Natural Science Foundation(S2013020013049)+1 种基金the Fundamental Research Funds for the Chinese Universities(2015PT002,2015ZP009)the Program of State Key Laboratory of Pulp and Paper Engineering(2015C04)~~
文摘A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles （PD-MNPs） was prepared and characterized. The widely used Aspetyillus niger lipase （ANL） was immobilized on the PD-MNPs （ANL@PD-MNPs） with a protein loading of 138 mg/g and an activity recovery of 83.6% under optimized conditions. For the immobilization, the pH and immobilization time were investigated. The pH and thermal and storage stability of the ANL@PD-MNPs significant- ly surpassed those of free ANL. The ANL@PD-MNPs had better solvent tolerance than free ANL. The secondary structure of free ANL and ANL@PD-MNPs was analyzed by infrared spectroscopy, A kinetic study demonstrated that the ANL@PD-MNPs had enhanced enzyme-substrate affinity and high catalytic efficiency. The ANL@PD-MNPs was applied as a biocatalyst for the regioselective acylation of dihydromyricetin （DMY） in DMSO and gave a conversion of 79.3%, which was higher than that of previous reports. The ANL@PD-MNPs retained over 55% of its initial activity after 10 cycles of reuse. The ANL@PD-MNPs were readily separated from the reaction system by a magnet. The PD-MNPs is an excellent support for ANL and the resulting ANL@PD-MNPs displayed good potential for the efficient synthesis of dihydromyricetin-3-acetate by enzymatic regioselective acylation.
基金Supported by the National Natural Science Foundation of China(Nos.41076031,41306190)the Ocean Public Welfare Industry Special Funds for Scientific Research(No.200905004-7)
文摘Settling particulate matter(SPM) is widely used to describe the sedimentary environment.We have investigated here the SPM collected with a time-series sediment trap in the Heini Bay,Weihai,Shandong Peninsula,China,during the super typhoon Muifa(August 2011,maximum wind speed 55 m/s).Meteorological and hydrological parameters were measured for 18 days.By analyzing the particle flux,grain size,and loss-on-ignition(organic matter content) of SPM we found dramatic changes in these parameters,induced by typhoon Muifa for about 6 days.With the arrival of typhoon,the daily SPM fluxes increased sharply to the maximum at 76.4 g/(m^2·d) on the first day and this increase is about 9 times of the normal value,and after 6 days the SPM decreased to the normal value gradually.Other parameters,such as grain size of SPM and organic matter also experienced similar changes.Using these SPM parameters,we divided the settling progress by cluster analysis into three phases:strong(for 3 days),weak(for 3 days),and zero typhoon impact.
