选取黑褐新糠虾为实验动物,采用半静态暴露法开展研究了As^(3+)、Cr^(6+)、Ni^(2+)毒性效应。急性毒性实验结果表明,As^(3+)、Cr^(6+)、Ni^(2+)对黑褐新糠虾的96 h LC50分别为92.04μg/L、233.20μg/L、537.10μg/L;相关抗氧化酶系统研...选取黑褐新糠虾为实验动物,采用半静态暴露法开展研究了As^(3+)、Cr^(6+)、Ni^(2+)毒性效应。急性毒性实验结果表明,As^(3+)、Cr^(6+)、Ni^(2+)对黑褐新糠虾的96 h LC50分别为92.04μg/L、233.20μg/L、537.10μg/L;相关抗氧化酶系统研究结果显示,SOD和CAT两种酶对三种重金属的暴露均呈现先诱导后抑制趋势。暴露结果显示,各处理组MDA水平显著高于对照组。综合3种重金属离子对黑褐新糠虾毒性数据,3种重金属离子毒性依次为As^(3+)>Cr^(6+)>Ni2,且氧化压力损伤被认为是其主要致毒机制之一。展开更多
The effects of Prorocentrum donghaiense, Karenia mikimotoi, Alexandrium catenella, the causative species of Harmful Algal Blooms in the East China Sea, on the survival and growth of Neomysis awatschensis and Artemia s...The effects of Prorocentrum donghaiense, Karenia mikimotoi, Alexandrium catenella, the causative species of Harmful Algal Blooms in the East China Sea, on the survival and growth of Neomysis awatschensis and Artemia salina were investigated. And the toxicity of eleven strains of Alexandrium——A. lusitanicum(AL-1)、A. minutum(AM-1)、A. spp.(A-K)、A. tamarense(ATHK,ATCI02, ATCI03, AT5-1, AT5-3,AT-6)、A. affine(AS-1,AC-1) to the two above organisms were compared.The results showed that P. donghaiense and K. mikimotoi had no adverse effect on the survival and growth of N. awatschensis and A. salina at 96h.However, the survival rate of N. awatschensis and A. salina declined linearly with the increasing density of A. catenella, and the 96h LC50 for N. awatschensis and A. salina was 4500 and 460cells/mL, respectively.Five strains of Alexandrium——A. spp, A. lusitanicum(AL-1), A. tamarense(AT5-3, AT-6)and A.affine(AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis.But other six strains of Alexandrium——A.tamarense(AT5-1, ATHK, ATCI02, ATCI03), A.affine(AC-1)and A.minutum(AM-1)decreased the survival rate of N.awatschensis significantly. Two strains of Alexandrium——A. lusitanicum(AL-1)and A.tamarense(AT-6), at the density of 500cells/mL had no adverse effects on A.salina in 96h; while the other Alexandrium strains all had adverse effects on the survival of A.salina.A.salina could increase its length with the increasing density of P.donghaiense, K.mikimotoi and A.lusitanicum. The results indicate that P.donghaiense and K.mikimotoi have no significant effect on the survival of crustacean zooplankton in a short period.But several Alexandrium species/strains could influence the survival of crustacean zooplankton likely by non-PSP substances.A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.展开更多
文摘选取黑褐新糠虾为实验动物,采用半静态暴露法开展研究了As^(3+)、Cr^(6+)、Ni^(2+)毒性效应。急性毒性实验结果表明,As^(3+)、Cr^(6+)、Ni^(2+)对黑褐新糠虾的96 h LC50分别为92.04μg/L、233.20μg/L、537.10μg/L;相关抗氧化酶系统研究结果显示,SOD和CAT两种酶对三种重金属的暴露均呈现先诱导后抑制趋势。暴露结果显示,各处理组MDA水平显著高于对照组。综合3种重金属离子对黑褐新糠虾毒性数据,3种重金属离子毒性依次为As^(3+)>Cr^(6+)>Ni2,且氧化压力损伤被认为是其主要致毒机制之一。
文摘The effects of Prorocentrum donghaiense, Karenia mikimotoi, Alexandrium catenella, the causative species of Harmful Algal Blooms in the East China Sea, on the survival and growth of Neomysis awatschensis and Artemia salina were investigated. And the toxicity of eleven strains of Alexandrium——A. lusitanicum(AL-1)、A. minutum(AM-1)、A. spp.(A-K)、A. tamarense(ATHK,ATCI02, ATCI03, AT5-1, AT5-3,AT-6)、A. affine(AS-1,AC-1) to the two above organisms were compared.The results showed that P. donghaiense and K. mikimotoi had no adverse effect on the survival and growth of N. awatschensis and A. salina at 96h.However, the survival rate of N. awatschensis and A. salina declined linearly with the increasing density of A. catenella, and the 96h LC50 for N. awatschensis and A. salina was 4500 and 460cells/mL, respectively.Five strains of Alexandrium——A. spp, A. lusitanicum(AL-1), A. tamarense(AT5-3, AT-6)and A.affine(AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis.But other six strains of Alexandrium——A.tamarense(AT5-1, ATHK, ATCI02, ATCI03), A.affine(AC-1)and A.minutum(AM-1)decreased the survival rate of N.awatschensis significantly. Two strains of Alexandrium——A. lusitanicum(AL-1)and A.tamarense(AT-6), at the density of 500cells/mL had no adverse effects on A.salina in 96h; while the other Alexandrium strains all had adverse effects on the survival of A.salina.A.salina could increase its length with the increasing density of P.donghaiense, K.mikimotoi and A.lusitanicum. The results indicate that P.donghaiense and K.mikimotoi have no significant effect on the survival of crustacean zooplankton in a short period.But several Alexandrium species/strains could influence the survival of crustacean zooplankton likely by non-PSP substances.A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.
