AIM: To investigate the reliability of massive hepatectomy models by using clip techniques. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The i...AIM: To investigate the reliability of massive hepatectomy models by using clip techniques. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.RESULTS: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques. CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.展开更多
Experiments were performed to study the action of fractionated irradiation of mice heads with γ-rays and protons on their peripheral blood parameters and behavior by the ＂Open Field＂ test. Mice were irradiated in t...Experiments were performed to study the action of fractionated irradiation of mice heads with γ-rays and protons on their peripheral blood parameters and behavior by the ＂Open Field＂ test. Mice were irradiated in two variants of fractionated irradiation： （1） traditional fractionation （in radiation therapy）： 2 Gy once a day, 5 times a week, the total radiation dose 20 Gy; （2） extreme hypofractionation： 10 Gy once a week, on Mondays, the total radiation dose 20 Gy. The results of the study showed that irradiation of mice heads has no effect on their peripheral blood parameters in both variants of the applied fractionated irradiation and the behavior of mice does not depend on the type of ionizing radiation and the variant of fractionated exposure that we used. On the basis of these results it can be concluded that the option of extreme hypofractionation we have chosen can successfully replace traditional fractionation, which in some cases is applied when carrying out radiotherapy for treating brain tumors. The application of this type of fractionation can lead to shorter terms of radiotherapy and bigger patient capacity of medical centers that conduct radiotherapy.展开更多
Label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods.Herein,we used a new strategy:data-dependent acquisition mode identification c...Label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods.Herein,we used a new strategy:data-dependent acquisition mode identification combined with label-free quantification by SWATH acquisition mode,to study the differentially expressed proteins in mouse liver cancer metastasis cells.A total of 1528 protein groups were identified,among which 1159 protein groups were quantified and 249 protein groups were observed as differentially expressed proteins(86 proteins up-regulated and 163 down-regulated).This method provides a commendable solution for the identification and quantification of differentially expressed proteins in biological samples.展开更多
基金Supported by Grants to Justin H Nguyen from the Deason Foundation, Sandra and Eugene Davenport, Mayo Clinic CD CRT-II and NIH R01NS051646-01A2partial grant to Tomohide Hori from the Uehara Memorial Foundation, No. 200940051, Tokyo 171-0033, Japan
文摘AIM: To investigate the reliability of massive hepatectomy models by using clip techniques. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.RESULTS: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques. CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.
文摘Experiments were performed to study the action of fractionated irradiation of mice heads with γ-rays and protons on their peripheral blood parameters and behavior by the ＂Open Field＂ test. Mice were irradiated in two variants of fractionated irradiation： （1） traditional fractionation （in radiation therapy）： 2 Gy once a day, 5 times a week, the total radiation dose 20 Gy; （2） extreme hypofractionation： 10 Gy once a week, on Mondays, the total radiation dose 20 Gy. The results of the study showed that irradiation of mice heads has no effect on their peripheral blood parameters in both variants of the applied fractionated irradiation and the behavior of mice does not depend on the type of ionizing radiation and the variant of fractionated exposure that we used. On the basis of these results it can be concluded that the option of extreme hypofractionation we have chosen can successfully replace traditional fractionation, which in some cases is applied when carrying out radiotherapy for treating brain tumors. The application of this type of fractionation can lead to shorter terms of radiotherapy and bigger patient capacity of medical centers that conduct radiotherapy.
基金financial support from the National Basic Research Program of China(2012CB910602,92013CB911200)the National Natural Science Foundation of China(2100507,21235005)+1 种基金the Creative Research Group Project by NSFC(21021004)the National High Technology Research and Development Program of China(2012AA020202)
文摘Label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods.Herein,we used a new strategy:data-dependent acquisition mode identification combined with label-free quantification by SWATH acquisition mode,to study the differentially expressed proteins in mouse liver cancer metastasis cells.A total of 1528 protein groups were identified,among which 1159 protein groups were quantified and 249 protein groups were observed as differentially expressed proteins(86 proteins up-regulated and 163 down-regulated).This method provides a commendable solution for the identification and quantification of differentially expressed proteins in biological samples.
