常德市1984～2007年鼠疫监测分析

目的分析常德市1984～2007年鼠疫监测结果,为持续做好鼠防工作提供科学依据。方法在常德市鼠疫历史疫源地连续24年开展人间鼠疫和动物鼠疫监测,并对监测结果进行分析。结果1984～2007年共捕鼠28412只,总捕鼠率为8.25%(4.04%～17.57%),获6个鼠种,褐家鼠占94.99%,为优势鼠种;1990年和1991年应用放射免疫法检出鼠疫F1抗体阳性褐家鼠3只,总阳性检出率为1.06/万;采集健康人群血清1026份,检测鼠疫F1抗体,结果均为阴性;1984～1988年梳检鼠516只,带蚤鼠22只,带蚤率4.26%,平均蚤指数为0.26匹。结论常德市存在鼠间鼠疫流行的可能,必须进一步加强鼠疫监测,落实各项防控措施。

Effects of Different Extract of Pseudostellaria Heterophylla on Immunological Function in Mice based on Meta-analysis and Network Meta-analysis

To evaluate the effects of different extract of Pseudostellaria heterophylla on immunological function in mice based on Meta-analysis and Network meta-analysis,the article retrieved domestic and foreign databases according to the"PICO"retrieval strategy,and used Stata and ADDIS software for Meta analysis.A total of 6 reports,10 randomised controlled trials(RCTs)were eventually involved.Meta analysis results showed that:compared with the control group,the experimental group of polysaccharide,saponin and crude extract were better than that of the control(P<0.05),which significantly improved the immunological function in mice.Network meta-analysis results showed that the saponin had the best effect on the increase of phagocytic index and the differences were statistically significant[MD=1.50,95%CI(0.71,2.28),P<0.05];The polysaccharide and saponin had better effect on the increase of spleen index than the control and crude extract,and the differences were statistically significant[MD=0.77,95%CI(0.27,1.31),P<0.05],[MD=0.71,95%CI(0.01,1.50),P<0.05];the polysaccharide had the best effect on the increase of thymus index and the differences were statistically significant[MD=0.70,95%CI(0.11,1.34),P<0.05].The rank probability showed that the saponin of Pseudostellaria heterophylla had the maximum probability to increase phagocytic index of mice;the probability for the components of Pseudostellaria heterophylla increasing spleen index of mice was in the order of crude extract>polysaccharide>saponin;the probability for the components of Pseudostellaria heterophylla increasing thymus index of mice was in the order of saponin>polysaccharide>crude extract.Based on the available evidence,the extract of Pseudostellaria heterophylla could improve the immunity of mice,and the clinical effect of polysaccharide and saponin was the best,which provided a more valuable scientific reference for evidencing that the polysaccharide and saponin of Pseudostellaria heterophylla was the effective components for improving immunological function,and also was conducive to the proper further development of Pseudostellaria heterophylla resources.

Murine brain tissues with human cytomegalovirus infection: a proteomic study

To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus（HCMV） infection and paired murine brain tissues and to identify the differential expression proteins. Methods： Forty Kunming mice were randomly divided into infection group （20） injected with HCMVAD169 and control group （20） injected with saline into their brain. After 30 days, the murine brain tissues by HCMV infection and paired murine brain tissues were separated by two-dimensional gel electrophoresis（2-DE）, analyzed by Image Master 2D software, and identified by peptide mass fingerprint（PMF） and database searching, and make Western blotting analyses the differential expression of individual proteins. Results： Well resolved, reproducible 2-D maps of the above tissues were obtained. Some of the different proteins identified by mass spectrometry（MS） were matched in the SWISS-2D PAGE database, Western blotting analyses were further carried out to verify the differential expression of individual proteins. Conclusion： These data will be valuable for studying the diagnosis of disease at an early stage, mechanisms of pathogenic and the key to the development of anti-HCMV medicine.

Increased telocytes involved in the proliferation of vascular smooth muscle cells in rat carotid artery balloon injury

Telocytes （TCs） are a novel type of interstitial cells that are thought to be involved in tissue regeneration and repair. However, the possible roles of TCs in vascular diseases remain unclear. In this study, we used a rat model of carotid artery balloon injury （CABI） to study the changes and potential roles of vascular TCs after vascular injury. Transmission electron microscopy （TEM） and CD34/vimentin immunolabeling were used to identify and quantify TCs in normal and injured carotid arteries. Quantita- tive immunofluorescence analysis revealed that, compared with the sham group, the number of TCs in the CAB1 group in- creased from 7.2＋1.0 to an average of 20.4＋1.8 per l-ram2 vascular area. The expression level of miR-24 in TCs was three times higher than in vascular smooth muscle cells （VSMCs）. The percentage of VSMCs in S phase and G2/M phase increased by approximately 5% when VSMCs were incubated with the supematant of TCs. The antagomir of miR-24 in TCs reduced the ratio of VSMCs in S ohase and G2/M phase. This study illuminates the function of TCs in the proliferation of VSMCs.